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Characterization of Graphene Oxide Suspension for Fluorescence Quenching in DNA-Diagnostics
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 Title & Authors
Characterization of Graphene Oxide Suspension for Fluorescence Quenching in DNA-Diagnostics
Kapitonov, A.N.; Alexandrov, G.N.; Vasileva, F.D.; Smagulova, S.A.; Timofeev, V.B.; Maksimova, N.R.; Kuznetsov, A.A.;
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The graphene oxides (GOs) were tested as a fluorescent quencher in the field of DNA-diagnostics. The various suspensions of GO nanoplates were prepared by changing the synthesis conditions. The suspensions were stable for at least 6 weeks by differing degrees of functionalization of various oxygen-containing groups of atoms. Depending on the properties of GO nanoplates, their fluorescent quenching abilities, which were determined by the amount of the tagged immobilized oligonucleotide, were also changed. GO suspension synthesized at of reaction mixture showed the fluorescent quenching of 16.39 nmol/mg, which would be a potential substitution of molecular fluorescent quencher in test-systems for DNA-diagnostics.
graphene oxide;DNA diagnostics;fluorescent quencher;
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