Diversity of Mycotoxigenic Fusarium armeniacum Isolated from Rice Grains at Harvest Time in Korea Hong, Sung Kee; Lee, Soohyung; Lee, Theresa; Ham, Hyeonheui; Mun, Hye Yeon; Choi, Hyo Won; Son, Seung-Wan; Ryu, Jae-Gee;
A total of 509 rice panicle samples were collected at harvest time from fields in 8 provinces from 2010 to 2014. One hundred five grains per sample were plated on potato dextrose agar and 6,658 Fusarium isolates were obtained; among them, 67 were identified as Fusarium armeniacum by sequencing the translation elongation factor () and confirmed by their morphological and cultural characteristics. Considerable variation in conidial size, colony color and sequences was observed among the fungal isolates. The ability of 24 F. armeniacum isolates to produce T-2 and HT-2 toxin in potato sucrose agar was determined using liquid chromatography-mass spectrometry. Twenty one isolates produced T-2 and HT-2 toxin, resulting in varying toxin levels among the isolates. The results show that Korean isolates of F. armeniacum have diversity with respect to morphological, cultural, genetic, and toxigenic properties.
Lee T, Lee S, Kim LH, Ryu JG. Occurrence of fungi and Fusarium mycotoxins in the rice samples from rice processing complexes. Res Plant Dis 2014;20:289-94.
Ok HE, Kim DM, Kim D, Chung SH, Chung MS, Park KH, Chun HS. Mycobiota and natural occurrence of aflatoxin, deoxynivalenol, nivalenol and zearalenone in rice freshly harvested in South Korea. Food Control 2014;37:284-91.
Lee J, Chang IY, Kim H, Yun SH, Leslie JF, Lee YW. Genetic diversity and fitness of Fusarium graminearum populations from rice in Korea. Appl Environ Microbiol 2009;75:3289-95.
Lee YW, Kim JG, Chung DH, Roh PU, Pestka JJ. Natural occurrence of zearalenone in rice and soybean produced in Korea. Mycotoxin Res 1991;7:69-72.
Ok HE, Kang YW, Kim M, Chun HS. T-2 and HT-2 toxins in cereals and cereal-based products in South Korea. Food Addit Contam Part B Surveill 2013;6:103-9.
Lee S, Kim M, Oh S, Chun HS. Trends in researches of Fusarium mycotoxins, T-2 toxin and HT-2 toxin in domestic and foreign countries. J Food Hyg Saf 2012;27:1-17.
Proctor RH, Hohn TM, McCormick SP. Reduced virulence of Gibberella zeae caused by disruption of a trichothecene toxin biosynthetic gene. Mol Plant Microbe Interact 1995;8:593-601.
O'Donnell K, Kistler HC, Cigelnik E, Ploetz RC. Multiple evolutionary origins of the fungus causing Panama disease of banana: concordant evidence from nuclear and mitochondrial gene genealogies. Proc Natl Acad Sci USA 1998;95:2044-9.
Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 1997;25:4876-82.
Burgess LW, Forbes GA, Windels C, Nelson PE, Marasas WF, Gott KP. Characterization and distribution of Fusarium acuminatum subsp. Armeniacum subsp. nov. Mycologia 1993;85: 119-24.
Burgess LW, Summerell A, Backhouse D, Benyon F, Levic J. Biodiversity and population studies in Fusarium. Sydowia 1996;48:1-11.
Ellis ML, Diaz Arias MM, Leandro LF, Munkvold GP. First Report of Fusarium armeniacum causing seed rot and root rot on soybean (Glycine max) in the United States. Plant Dis 2012;96:1693.2.
Wang Y, Lu BH, Yang LN, Gao J. First report of Fusarium armeniacum causing stem and root rot on Platycodon grandiflorum in Jilin province, China. Plant Dis 2015. http://dx.doi.org/10.1094/PDIS-01-15-0108-PDN.