First Report of Gray Mold Disease of Sweet Basil (Ocimum basilicum) Caused by Botrytis cinerea in Korea Aktaruzzaman, Md.; Kim, Joon-Young; Afroz, Tania; Hong, Sae-Jin; Kim, Byung-Sup;
In August 2015, we collected samples of gray mold from sweet basil growing in Sachunmeon, Gangneung, Gangwon Province, Korea. Symptoms included extensive growth of mycelia with gray conidia on young leaves, stems, and blossoms. The pathogen was isolated from infected leaves and blossoms and the fungus was cultured on potato dextrose agar. For identification of the fungus, morphology and rDNA sequencing analysis of the fungus were performed, which confirmed its pathogenicity according to Koch's postulates. The results of morphological examinations, pathogenicity tests, and the rDNA sequences of the internal transcribed spacer regions (ITS1 and ITS4) and the three nuclear protein-coding genes G3PDH, HSP60, and RPB2 showed that the causal agent was Botrytis cinerea. This is the first report of gray mold caused by Botrytis cinerea on sweet basil in Korea.
Guenther E. The essential oils, v. 3: individual essential oils of the plant families. New York: D. Van Nostrand; 1949.
Makri O, Kintzios S. Ocimum sp. (Basil): botany, cultivation, pharmaceutical properties, and biotechnology. J Herbs Spices Med Plants 2008;13:123-50.
Elad Y. Effect of filtration of solar light on the production of conidia by field isolates of Botrytis cinerea and on several diseases of greenhouse crops. Crop Prot 1997;16:635-42.
Jarvis WR. Botryotinia and Botrytis species: taxonomy, physiology and pathogenicity. Ottawa: Research Branch, Canada Department of Agriculture; 1977.
Agricultural Research Service. Fungal Databases [Internet]. Washington D.C.: United States Department of Agriculture; 2015 [cited 2015 Nov 8]. Avaiable from: http://nt.ars-grin.gov/fungaldatabases/.
White TJ, Bruns TD, Lee SB, Taylor JW. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, editors. PCR protocols: a guide to methods and applications. San Diego: Academic Press; 1990. p. 315-22.
Staats M, van Baarlen P, van Kan JA. Molecular phylogeny of the plant pathogenic genus Botrytis and the evolution of host specificity. Mol Biol Evol 2005;22:333-46.
Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 2011;28:2731-9.
Ellis MB, Waller JM. Sclerotinia fuckeliana (conidial state: Botrytis cinerea). In: Commonwealth Mycological Institute (Great Britain), CAB International, editors. CMI descriptions of pathogenic fungi and bacteria. Wallingford: CAB International; 1974. no.431.