Advanced SearchSearch Tips
Digestion indices of 12 species of microalgae by the oyster Crassostrea gigas larval development stages
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
  • Journal title : The Korean Journal of Malacology
  • Volume 27, Issue 4,  2011, pp.359-369
  • Publisher : The Malacological Society of Korea
  • DOI : 10.9710/kjm.2011.27.4.359
 Title & Authors
Digestion indices of 12 species of microalgae by the oyster Crassostrea gigas larval development stages
Hur, Young-Baek; Jeon, Chang-Young; Cho, Kee-Chae; Hur, Sung-Bum;
  PDF(new window)
Twelve species of food microalgae were investigated to clarify the digestion index of Crassostrea gigas larvae using epifluorescence microscopy to choose an appropriate diet for artificial seed production in hatchery. An experiment was conducted using 1 (D shaped stage), 4 (Early umbo stage), 8 (umbo stage) and 12 (Full grown stage) days old larvae. larvae were stocked in 1 L flasks at 5 individuals/mL and fed algal cells/mL of each species individually. Prior to larvae were fed for 3 h and then were observed under the microscope to detect ingestion; larvae were then sieved and replaced in 1 L flasks containing filtered seawater and were observed after 3, 5 and 8 h to analyse the digestion index. Values of digestion indices were specific for each alga. No evidence for the ingestion of Thalassiosira weissflogii was evident at all larval development stages tested. Digestion indices of others microalgae were 0.8-99.7% at 4 stage of larval development stages: Chlorella ellipsoidea (0.8-5.4%), Nannochloris oculata (1.4-5.0%), Isochrysis galbana (99.1-99.5%), Pavlova lutheri (99.1-99.5%), I. aff. galbana (99.4-99.5%), Cheatoceros calcitrans (0.0-99.2%), C. gracilis (0.0-99.7%), C. simplex (0.0-95.9%), Phaeodactylum tricornutum (0.0-99.6%), Tetraselmis tetrathele (0.0-99.7%) and Dunaliella tertiolecta (0.0-99.6%), respectively. Therefore, it is assumed that food microalgae showing the high digestion such as I. galbana should be supplied to the early umbo stage larvae, and then after the umbo larval stage, the mixed microalgae with diatoms and light green algae should be supplied to the full grown stage larvae to increase the digestion of their larvae.
Digestion index;Epifluorescence microscopy;oyster;Crassostrea gigas;microalgae;larval development;
 Cited by
Comparative genetic diversity of wild and hatchery-produced Pacific oyster (Crassostrea gigas) populations in Korea using multiplex PCR assays with nine polymorphic microsatellite markers,;;;;;;;;;;

Genes and Genomics, 2013. vol.35. 6, pp.805-815 crossref(new window)
Comparative genetic diversity of wild and hatchery-produced Pacific oyster (Crassostrea gigas) populations in Korea using multiplex PCR assays with nine polymorphic microsatellite markers, Genes & Genomics, 2013, 35, 6, 805  crossref(new windwow)
Abdel-Hamid, M.E., Mona, M,H., and Khalil, A,M. (1992) Effects of temperature, food and food concentrations on the growth of the larvae and spat of the edible oyster Crassostrea gigas (Thunberg). Journal of Marine Biology Association, 34: 195-202.

Albentosa, M., Perez-Camacho, A., Labarta, U., Beiras, R. and Fernandez-Reiriz, M.J. (1993) Nutritional value of algal diets to clam spat Venerupis pullastra. Marine Ecology Progress Service, 97: 261-269. crossref(new window)

Albentosa, M., Fernandez-Reiriz, M.J., Perez-Camacho, A. and Labarta, U. (1999) Growth performance and biochemical composition of Ruditapes decussatus (L.) spat fed on microalgal and wheatgerm flour diets. Journal of Experimental Marine Biology and Ecology, 232: 23-37 crossref(new window)

Aldana-Aranda, D., Lucas, A., Brulé, T., Andrade, M., García, E., Maginot, N. and Le Pennec, M. (1991) Observations on ingestion and digestion of unicellular algae by Strombus gigas larvae (Mollusca, Gastropoda) using epifluorescence microscopy. Aquaculture, 92: 359-366. crossref(new window)

Aldana-Aranda, D., Patino-Suarez, V. and Brule, T. (1994) Ingestion and digestion of eight algae by Strombus gigas larvae (Mollusca, Gastropoda) Studied by epifluorescence microscopy. Aquaculture, 126: 151-158. crossref(new window)

Aldana-Aranda, D., Patino-Suarez, V. and Brule, T. (1997) Nutritional potentialities of Chlamydomonas coccoides and Thalassiosira fluviatilis, as measured by their ingestion and digestion rates by the Queen Conch larvae (Strombus gigas). Aquaculture, 156: 9-20. crossref(new window)

Babinchak, J. and Ukeles, R. (1979) Epifluorescence microscopy, a technique for the study of feeding in Crassostrea virgtnica veliger larvae. Marine Biology, 51: 69-76. crossref(new window)

Badillo-Salas, C.E., Valenzuela-Espinoza, E., Gonzalez-Gomez, M.A., Pares-Sierra, G., Ley-Lou, F. and Garcia-Esquivel, Z. (2009) Comparative growth of Pacific oyster (Crassostrea gigas) postlarvae with microfeed and microalgal diets. Aquaculture International, 17: 173-186. crossref(new window)

Baldwin, B.S. and Newell, R.I.E. (1995) Feeding rate responses of oyster larvae (Crassostrea virginica) to seston quantity and composition. Journal of Marine Biology Association, 189: 77-91.

Ben-Amotz, A. and Avron, M. (1980) The biotechnology of cultivating the halotolerant alga Dunaliella. Trends in Biotechnology. pp. 121-126. lenum Press. New York.

Bayne, B.L. (1983) Physiological ecology of marine molluscan larvae. In: Verdonk NH, eds, The Mollusca, vol. III. pp. 299-343. Academic Press. New York.

Coutteau, P. and Sorgeloos, P. (1992) The use of algal substitutes and the requirement for live algae in hatchery and nursery rearing of bivalve molluscs: An international survey. Journal of Shellfish Research, 11: 467-476.

Devakie, M.N. and Ali, A.B. (2000) Salinity-temperature and nutritional effects on the setting rate of larvae of the tropical oyster, Crassostrea iredalei (Faustino). Aquaculture, 184: 105-114. crossref(new window)

Dunstan, G.A., Volkman, J.K., Jeffrey, S.W. and Barret, S.M. (1992) Biochemical composition of microalgae from the green algal classes Chlorophyceae and Prasinophyceae. 2. Lipid classes and fatty acids. Journal of Experimental Marine Biology and Ecology, 161: 115-134. crossref(new window)

Elston, R. (1980a) Functional morphology of the coelomocytes of the larval oysters (Crassostrea virginica and Crassostrea gigas). Journal of the Marine Biological Association of the united Kingdom, 60: 947-957. crossref(new window)

Elston, R. (1980b) Functional anatomy, histology and ultrastructure of the soft tissues of the larval American oyster, Crassostrea virginica. Proceeding of National Shellfish Association, 70: 65-93.

Espinosa, E.P. and Allam, B. (2006) Comparative growth and survival of juvenile hard clams, Mercenaria mercenaria, fed commercially available diets. Zoo Biology, 25: 503-525.

Enes, P. and Borges, M.T. (2003) Evaluation of microalgae and industrial cheese whey as diets for Tapes decussatus (L.) seed: effect on water quality, growth, survival, condition and filtration rate. Aquaculture Research, 34: 299-309. crossref(new window)

Ewart, J.W. and Epifanio, C.E. (1981) A tropical flagellate food for larval and juvenile oysters, Crassostrea virginica Gmelin. Aquaculture 22: 297-300. crossref(new window)

Floyd, D.J. (1953) Foods and feeding of oysters as observed with the use of radioactive plankton. Proceeding of National Shellfish Association Convention Addresses, 71-180.

Gallager, S.M. (1988) Visual observations of particle manipulation during feeding in larvae of a bivalve mollusk. Bulletin of marine Science, 43: 344-365.

Gerdes, D. (1983) The Pacific oyster Crassostrea gigas. Part I. Feeding behavior of larvae and adults. Aquaculture, 31: 195-219. crossref(new window)

Helm, M.M. and Millican, P.F. (1977). Experiments in the hatchery of Pacific oyster (Crassostrea gigas Thunberg). Aquaculture, 11: 1-12. crossref(new window)

His, E., Robert, R. and Dinet, A. (1989) Combined effects of temperature and salinity on fed and starved larvae of the Mediterranean mussel, Mytilus galloprovincialis and the Japanese oyster Crassostrea gigas. Marine Biology, 100: 455-463. crossref(new window)

Lemos, M.B.N., Nascimento, I.A., De Araujo, M.M.S., Pereira, S.A., Bahia, I. and Smith, D.H. (1994) The combined effects of salinity, temperature, antibiotics and aeration on larval growth and survival of the mangrove oyster, Crassostrea rhizophorae. Journal of Shellfish Research, 13: 187-192.

Lucas, A. and Rangel, D.C. (1983) Detection of the first larval feeding in Crassostrea gigas, using epifluorescence microscope. Aquaculture, 30: 369-374. crossref(new window)

Myers, J.A. and Boisvert, R.N. (1990) The economics of producing algae and bivalve seed in hatcheries. Aquaculture, 86: 163-179. crossref(new window)

Owen, G. (1974). Feeding and digestion in the bivalvia. In: Lowenstein O. ed, Advances in Comparative Physiology and Biochemistry, vol. 5. pp. 1-35. Academic Press. New York, USA.

Ponis, E., Robert, R., Parisi, G. and Tredici, M. (2003a). Assessment of the performance of Pacific oyster (Crassostrea gigas) larvae fed with fresh and preserved Pavlova lutheri concentrates. Aquaculture International, 11:69-79. crossref(new window)

Ponis, E., Robert, R. and Parisi, G. (2003b) Nutritional value of Pavlova lutheri, Isochrysis aff. galbana clone T-Iso and Chaetoceros calcitrans forma pumilum, either fresh or preserved, for larval and post-larval development of Pacific oyster (Crassostrea gigas). Aquaculture, 221: 491-505. crossref(new window)

Rico-Villa, B., Le Coz, J.R., Mingant, C. and Robert, R. (2006) Influence of phytoplankton diet mixtures on microalgae consumption, larval development and settlement of the Pacific oyster Crassostrea gigas (Thunberg). Aquaculture, 256: 377-388. crossref(new window)

Rico-Villa, R., Pouvreau, S. and Rober, R. (2009) Influence of food density and temperature on ingestion, growth and settlement of Pacific oyster larvae, Crassostrea gigas. Aquaculture, 287: 395-401.

Robert, R. and Trintignac, P. (1997) Substitutes for live microalgae in mariculture: a review. Aquatic Living Resources, 10: 315-327. crossref(new window)

Urban, E.R. and Langdon, C.J. (1984) Reduction in costs of diets for the American oyster, Crassostrea virginica (Gmelin), by the use of non-algal supplements. Aquaculture, 38: 277-291. crossref(new window)

Webb, K.L. and Chu, F.E.. (1982) Phytoplankton as a food source for bibalve larvae. in Pruder GD, Langdon CJ and Conklin DE. eds, Second International Conference on Aquaculture Nutrition: Biochemical and Physiological Approaches to Shellfish Nutrition, 272-291. Louisiana State University. Baton Rouge.

Walne, P.R. (1974) Culture of bivalve molluscs, 173. The whitefriars Press Ltd. London and Tondridge.

Walne, P.R. (1976) Factors affecting the relation between feeding and growth in bivalves. Devik O. eds, Harvesting Polluted Waters, 169-183. Plenum Press. New York.

Wisely, B. and Reid, B. (1978) Experimental feeding of sydney rock oysters (Carssostrea commercialis = Saccostrea cucullata): I. optimum particle sizes and concentrations. Aquaculture, 15: 319-331. crossref(new window)