JOURNAL BROWSE
Search
Advanced SearchSearch Tips
Cytogenetic Analysis of the Triploid Pacific Abalone, Haliotis discus hannai
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
  • Journal title : The Korean Journal of Malacology
  • Volume 28, Issue 1,  2012, pp.37-43
  • Publisher : The Malacological Society of Korea
  • DOI : 10.9710/kjm.2012.28.1.037
 Title & Authors
Cytogenetic Analysis of the Triploid Pacific Abalone, Haliotis discus hannai
Jee, Young-Ju; Chang, Young-Jin;
  PDF(new window)
 Abstract
In this study, we invesgated a cytogenetic analysis of the Pacific triploid abalone, Haliotis discus hannai induced by low temperature treatment. We got a lot of mitotic metaphase chromosome spreads from the triploid and diploid Pacific abalones' hatched larvae (trochophores). The chromosome number of diploid abalone was 2n = 36 and that of triploid abalone was 3n = 54, so the chromosome number of triploid abalone was 1.5 times higher than that of diploid abalone. We developed a modified flow cytometric method for Pacific abalone from the existing methods. We uesd 51 months aged triploid and diploid Pacific abalones' hemolymph to get the DNA contents by flow cytometry. The DNA content of diploid abalone was 1.743 pg/cell and the DNA content of triploid abalone was 1.49 times higher than that of diploid one. It proved that triploid abalone was consisted with two sets of maternal diploid and one set of paternal genome.
 Keywords
Pacific abalone;Haliotis discus hannai;Triploid;Chromosome number;Flow cytometry;DNA content;
 Language
Korean
 Cited by
1.
북방전복, Haliotis discus hannai 3배체의 생식능력,지영주;남보혜;이정용;장영진;

한국패류학회지, 2013. vol.29. 2, pp.105-111 crossref(new window)
1.
Maturity and Spawning of the Triploid Pacific Abalone, Haliotis discus hannai, The Korean Journal of Malacology, 2013, 29, 2, 105  crossref(new windwow)
 References
1.
Allen, Jr.S.K. (1983) Flow cytometry: assaying experimental polyploid fish and shellfish. Aquaculture, 33: 317-328. crossref(new window)

2.
Arai, K., Tsubaki, H., Ishitani, Y. and Fujino, K. (1982) Chromosomes of Haliotis discus hannai INO and H. discus REEVE. Bulletin of the Japanese of Society of Scientific Fisheries, 48: 1689-1691. crossref(new window)

3.
Beaumont, A.R. (1986) Genetic aspects of hatchery rearing of the scallop, Pecten maximus (L.). Aquaculture, 57: 99-110. crossref(new window)

4.
Beaumont, A.R. and Fairbrother, J.E. (1991) Ploidy manipulation in molluscan shellfish: a review. Jounal of Shellfish Research, 10: 1-18.

5.
Beaumont, A.R. and Contaris, M.H. (1988) Production of triploid embryos of Tapes semidecussatus by the use of cytochalasin B. Aquaculture, 73: 37-42. crossref(new window)

6.
Benfey, T.J. and Sutterlin, A.M. (1984) Triploidy induced by heat shock and hydrostatic pressure in Atlantic salmon (Salmo salar L.). Aquaculture, 36: 359-367. crossref(new window)

7.
Bihari, N., Micic, M., Batel, R. and Zahn, R.K. (2003) Flow cytometric detection of DNA cell cycle alterations in hemocytes of mussels (Mytilus galloprovincialis) off the Adriatic coast, Croatia. Aquatic Toxicology, 64: 121-129. crossref(new window)

8.
Chaiton, J.A. and Allen, Jr.S.K. (1985) Early detection of triploidy in the larvae of Pacific oyster, Crassostrea gigas, by flow cytometry. Aquaculture, 48: 35-43. crossref(new window)

9.
Chang, Y.J. (1993) Identification of blood cells and their physiological functions in the scallop, Patinopecten yessoensis. Journal of Aquaculture, 6: 1-12. [in Korean]

10.
Chevassus, B. (1983) Hybridization in fish. Aquaculture, 33: 245-262 crossref(new window)

11.
Ciudad, J., Cid, E., Velasco, A., Lara, J.M., Lara, J. and Orfao1, A. (2002) Flow Cytometry Measurement of the DNA Contents of G0/G1 Diploid Cells From Three Different Teleost Fish Species. Cytometry, 48: 20-25. crossref(new window)

12.
Downing, S.L. and Allen, Jr.S.K. (1987) Induced triploidy in the Pacific oyster, Crassostrea gigas: Optimal treatment with cytochalasin B depend on temperature. Aquaculture, 60: 1-15. crossref(new window)

13.
Iverson, O.E. and Laerum, O.D. (1987) Trout and salmon erythrocytes and human leukocytes as internal reference standards for ploidy control in flow cytometry. Cytometry, 8: 190-196. crossref(new window)

14.
Jakobsen, A. (1983) The use of trout erythrocytes and human lymphocytes for standardization in flow cytometry. Cytometry, 4: 161-165. crossref(new window)

15.
Jee, Y.J. and Chang, Y.J. (1995) Induction of triploid abalone, Haliotis discus hannai, and its biological characteristics I. Induction of triploid abalone. Journal of Aquaculture 8, 159-170. [in Korean]

16.
Jeong, C.H., Ahn J.H., Kim, B.S. and Kim, D.S. (1995) Mass production of all-female triploid eggs in rainbow trout (Oncorhynchus mykiss) during the fall season by chromosome manipulation. Journal of Aquaculture, 8: 141-148. [in Korean]

17.
Juchno, D., Lackowska, B., Boron, A. and Kilarski, W. (2010) DNA content of hepatocyte and erythrocyte nuclei of the spined loach (Cobitis taenia L.) and its polyploid forms. Fish Physiology and Biochemistry, 36: 523-529 crossref(new window)

18.
Kim, D.S., Jeong, C.H. Lee, Y.D. and Rho, S. (1994) Triploidy induction in oliver flounder, Paralichthys olivaceus. Journal of Aquaculture, 7: 55-61. [in Korean]

19.
Kim, D.S., Noh, C.H. and Nam, Y.K. (1993) Induction of tetraploid cyprinid loach, Misgurnus anguillicaudatus. Journal of Aquaculture, 6: 55-62. [in Korean]

20.
Komaru, A. and Wada, K.T. (1985) Karyotype of the Japanese pearl oyster, Pinctada fucata martensii, observed in the trochophore larvae. Bulletin of National Research Institute of Aquaculture, 7: 105-107.

21.
Komaru, A., Uchimura, Y., Ieyama, H. and Wada, K.T. (1988) Detection of induced triploid scallop Chlamys nobilis, by DNA microfluorimetry with DAPI staining. Aquaculture, 69: 201-209. crossref(new window)

22.
Lamatsch, D.K., Steinlein, C., Schmid, M. and Schartl, M (2000) Noninvasive determination of genome size and ploidy level in fishes by flow cytometry: detection of triploid Poecillia formosa. Cytometry, 39: 91-95. crossref(new window)

23.
Li, Y., Li, X. and Qin, J.G. (2007) Triploidy induction in Australian greenlip abalone Haliotis laevigata (Donovan) with cytochalasin B. Aquaculture Research, 38: 487-492. crossref(new window)

24.
Park, I.S. and Kim, H.B. (1994) Induction of triploid cherry salmon, Oncorhynchus masou. Journal of Aquaculture, 7: 207-223. [in Korean]

25.
Park, I.S. and Park, K.Y. (1995) Haematological and physiological characteristics of diploid and triploid in cherry salmon, Oncorhynchus masou. Journal of Aquaculture, 8: 21-29. [in Korean]

26.
Peruzz,i S. and Chatain, B. (2003) Induction of tetraploid gynogenesis in the European sea bass (Dicentrarchus labrax L.). Genetica, 119: 225-228. crossref(new window)

27.
Seeb J.E., Thorgaard, G.H. and Utter, F.M. (1988) Survival and allozyme expression in diploid and triploid hybrids between chum, chinook, and coho salmon. Aquaculture, 72, 31-48 crossref(new window)

28.
Stanley, J.G., Allen, Jr.S.K., and Hidu, H. (1981) Polyploidy induced in the American oyster, Crassostrea virginica, with cytochalasin B. Aquaculture, 23: 1-10. crossref(new window)

29.
Tabarini, C.L. (1984) Induced triploidy in the bay scallop, Argopecten irradians, and its effect on growth and gametogenesis. Aquaculture, 42: 151-160. crossref(new window)

30.
Tanaka, K., Takasugi, T. and Maoka, H. (1961) Morphological characteristics of the blood-corpuscles of the common oyster, Gryphea gigas. Bulletin of the Japanese of Society of Scientific Fisheries, 27: 365-371. crossref(new window)

31.
Thorgaard G.H., Rabinovitch, P.S., Shen, M.W., Gall, G.A.E., Propp, J. and Utter, F.M. (1982) Triploid rainbow trout identified by flow cytometry. Aquaculture, 29: 305-309. crossref(new window)

32.
Utter, F.M., Johnson, O.W. Thorgaard, G.H. and Rabinovitch, P.S. (1983) Measurment and potential applications of induced triploidy in pacific salmon. Aquaculture, 35: 125-135. crossref(new window)

33.
Wada, K.T., Komaru, A. and Uchimura, Y. (1989) Triploid induction in the Japanese pearl oyster, Pinctada fucata martensii. Aquaculture, 76: 11-19. crossref(new window)

34.
Wolters. W.R., Chrisman C.L. and Libey, G.S. (1982) Erythrocyte nuclear measurements of diploid and triploid channel catfish, Ictalurus punctatus (Rafinesque). Journal of Fish Biology, 20: 253-258. crossref(new window)

35.
Yamamoto, S. and Sugawara, Y. (1988) Induced triploidy in the mussel, Mytilus edulis, by temperature shock. Aquaculture, 72: 21-29. crossref(new window)

36.
Yang, H. and Guo, X. (2006) Polyploid induction by heat shock-induced meiosis and mitosis inhibition in the dwarf surfclam, Mulinia lateralis Say. Aquaculture, 252: 171-182. crossref(new window)

37.
박인석. (1992) 미꾸리와 미꾸라지의 잡종 및 잡종 3배체에 관한 연구. 부산수산대학교 대학원 박사학위 청구논문, 85pp.

38.
松谷武成․高橋計介.森 勝義. (1994) フロ-サイトメトリ-よるマガキ成貝の三倍體檢出のための簡易試料調整法. 第1回 韓.日 水産增養殖學術 Symposium 發表要旨集, 94pp

39.
赤繁悟․池田 實.藤尾芳久. (1992) アイソザイム遺傳子からみた三倍體マガキと二倍體マガキの差異. 水産育種, 18: 33-39.