JOURNAL BROWSE
Search
Advanced SearchSearch Tips
Quantification of Reproductive Effort and Microscopic Observation on the Larval Development of Manila clam Ruditapes philippinarum (Adams and Reeve, 1850)
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
  • Journal title : The Korean Journal of Malacology
  • Volume 28, Issue 2,  2012, pp.145-156
  • Publisher : The Malacological Society of Korea
  • DOI : 10.9710/kjm.2012.28.2.145
 Title & Authors
Quantification of Reproductive Effort and Microscopic Observation on the Larval Development of Manila clam Ruditapes philippinarum (Adams and Reeve, 1850)
Lee, Hee-Jung; Kang, Hyun-Sil; Park, Kyung-Il; Mondol, Mostafisur Rahman; Choi, Kwang-Sik;
  PDF(new window)
 Abstract
Larval development of the Manila clam Ruditapes philippinarum reared in an indoor tank system was examined in this study using light microscope and scanning electron microscope. To induce spawning and subsequent larval development, clams were collected from the intertidal zone at Gim-nyeong harbor in Jeju Island in August 2011. After 2 days of rearing in the tank, all Manila clams spawned in the midnight. Non-feeding trochophore larvae appeared 7hrsafter fertilization and the first D-shape larvae could be observed at 19 hrs. Twenty one days after fertilization the pediveliger larvae crawling on the bottom of the tank with well-developed foot were observed. Histology indicated that all the clams used in this study were in the ripe stage prior to spawning and the gonad-somatic index (GSI), a ratio of the egg mass to the tissue weight, of the ripe female measured by ELISA was 28.6%. The GSI of female clam declined to 17.3% after the massive spawning in the tank, suggesting that Manila clam discharged 40% of the total eggs during the first spawning event. In conclusion, spawning and subsequent larval development of Manila clam was successfully carried out in this study using an indoor tank system, and the information obtained in the present study could be useful in future Manila clam hatchery development.
 Keywords
Ruditapes philippinarum;larval development;reproductive effort;SEM;Histology;
 Language
Korean
 Cited by
1.
주사전자현미경 (Scanning Electron Microscope)을 이용한 제주 북부 연안에 서식하는 가시굴 (Saccostrea kegaki Torigoe & Inaba, 1981)의 초기 유생발달관찰,이희중;강현실;정희도;홍현기;최광식;

한국패류학회지, 2013. vol.29. 2, pp.97-103 crossref(new window)
1.
First observation on the early embryonic and larval development of spiny oyster Saccostrea kegaki Torigoe & Inaba, 1981 (Bivalvial: Ostreoida) using scanning electron microscope on the north coast of Jeju, Korea, The Korean Journal of Malacology, 2013, 29, 2, 97  crossref(new windwow)
 References
1.
Affan, M. A. (2006) Ecological and ecophysiological studies and application of microalgae on the west coast of Jeju Island. Ph.D. Dissertation., Dept. Oceanography, Cheju National University. 115 pp.

2.
Chung, E.Y., Hur, S.B., Lee, J.S. (2001) Gonadal maturation and artificial spawning of the Manila clam Ruditapes philippinarum (Bivalvia: Veneridae), in Komso bay, Korea. journal of Fisheries Science Technology, 4: 208-218.

3.
Drummond, L., Mulcahy, M., Culloty, S. (2006) The reproductive biology of Manila clam, Ruditapes philippinarum, from the North-West of Ireland. Aquaculture, 254: 326-340. crossref(new window)

4.
Hur, Y.B. (2005) Comparison of development and larval growth of four Venerid clams. Journal of the World Aquaculture Society, 36: 179-187.

5.
Healy, J.M. (1995) Comparative spermatozoal ultrastructure and its taxonomic and phylogenetic significance in the bivalve order Veneroida. In: Jamieson, B.G.M., Ausio, J., Justine, J.-L., (eds), Advances in spermatozoal phylogeny and taxonomy. Memoires Du Museum National D'Histoire Naturelle, 166: 155-166.

6.
Helm, M.M., Bourne, N. (2004) Hatchery operation: Broodstock conditioning, spawning, and fertilization. In: A. Lovatelli, editor. Hatchery culture of bivalve: A practical manual, FAO fisheries technical paper, Italy. 471 pp.

7.
Goulletquer, P. (1997) A bibliography of the Manila clam Tapes philippinarum. IFREMER, RIDRV-97.02/RA/La tremblade, 122p.

8.
Kang, C.K., Park, M.S., Lee, P.Y., Choi, W.J., Lee, W.C. (2000) Seasonal variation in condition, reproductive activity, and biochemical composition of Pacific oyster, Crassostrea gigas (Thunberg) in suspended culture in two coastal bays of Korea. Journal of Shellfish Research, 19: 771-778.

9.
Kang, C.K., Kang, Y.S., Choy, E.J., Kim, D.S., Shim, B.T., LEE, P.Y. (2007) Condition, reproductive activity, and gross biochemical composition of the Manila clam, Tapes Philippinarum in natural and newly created sandy habitats of the southern coast of Korea. Journal of Shellfish Research, 26: 401-412. crossref(new window)

10.
Kang, D.H., Ngo, T.T.T., Koh, K.J., Choi, S.K. (2004) Seasonal changes in gonadal development of Manila clam, Ruditapes philippinarum form Shi-Heung-Ri, Jeju, Korea. Journal of Aquaculture 4: 81-88 (In Korean).

11.
Kang, D.H., Chu, F.E., Yang, H.S., Lee, C.H., Koh, H.B., Choi, K.S. (2010) Growth, reproductive condition, and digestive tubule atrophy of Pacific oyster Crassostrea gigas in Gamakman Bay off the southern coast of Korea. Journal of Shellfish Research, 29: 839-845. crossref(new window)

12.
Kim, D.S., Lim, D.I., Jeon, S.K., Jung, H.S. (2005) Chemical characteristics and eutrophication in Cheonsu Bay, west coast of Korea. Ocean Polar Research, 27: 45-58. crossref(new window)

13.
KOSIS. (2003) Statistic Database for Reclamation Extent.(http:/kosis.kr/wnsearch/totalSearch.jsp)

14.
Limpanont, Y., Yang, H.S., Park, K.I., Choi, K.S. (2011) First report on the annual gametogenesis of Heteromacomairus (Hanley, 1845) in a rocky intertidal area, northern Jeju Island, Korea. Journal of Shellfish Research, 30: 39-46. crossref(new window)

15.
Min, D.K. (2004) Mollusks in Korea. Busan, Korea: Hangul Graphics. 465 pp.

16.
Ngo, T.T.T and Choi, K.S. (2004) Seasonal changes of Perkinsus and Cercaria infections in the Manila clam Ruditapes philippinarum from Jeju, Korea. Aquaculture 239: 57-68. crossref(new window)

17.
NFRDI. 2001. Development of optimal technology for sustaining production in shellfish farm. National Fisheries and Development Institute of Korea technical report, 116 pp.

18.
Menegghetti, F., Moschino, V., Da Ros, L. 2004.Gametogenic cycle and variations in oocyte size of Tapes philippinarum from the Lagoon of Venice. Aquaculture, 240: 473-488. crossref(new window)

19.
Park, K.I. and Choi, S.K. (2004) Application of enzyme-linked immunosorbent assay for studying of reproduction in the Manila clam Ruditapes philippinarum (Mollusca: Bivalvia) I. Quantifying eggs. Aquaculture, 241: 667-687.

20.
Ponurovsky, S.K and Yakovlev Y.M. (1992) The reproductive biology of the Japanese littleneck, Tapes philippinarum (A. Adams and Reeve, 1850) (Bivalvia: Veneridae). Journal of Shellfish Research, 11: 265-277.

21.
Toba, M., Miyama, Y., (1991) Gonadal development and spawning induction in artificially conditioned Manila clams Ruditapes philippinarum. Nippon Suisan Gakkaku, 57: 1269-1275 (In Japanese with English abstract). crossref(new window)

22.
Uddin, M.J., Yang, H.S., Choi, S.K. (2010) Seasonal changes on Perkinsus olseni infection and gametogenesis in Manila clam Ruditapes philippinarum, from Seonjaedo island on Incheon, off the west coast of Korea. journal of World Aquaculture, 41: 93-101. crossref(new window)

23.
Yap, G.W. (1977) Population biology of the Japanese little-neck clam, Tapes philippinarum, in Kaneohe bay, Oahu, Hawaiian Islands. Pacific Science, 31: 223-244.

24.
Zhang, G and Yan, X. (2006) A new three-phase culture method for Manila clam, Ruditapes philippinarum, farming in northern China. Aquaculture, 258: 452-461. crossref(new window)