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Expressed sequence tag analysis of Meretrix lusoria (Veneridae) in Korea
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  • Journal title : The Korean Journal of Malacology
  • Volume 28, Issue 4,  2012, pp.377-384
  • Publisher : The Malacological Society of Korea
  • DOI : 10.9710/kjm.2012.28.4.377
 Title & Authors
Expressed sequence tag analysis of Meretrix lusoria (Veneridae) in Korea
Kang, Jung-Ha; Jeong, Ji Eun; Kim, Bong Seok; An, Chel-Min; Kang, Hyun-Sook; Kang, Se-Won; Hwang, Hee Ju; Han, Yeon Soo; Chae, Sung-Hwa; Ko, Hyun-Sook; Lee, Jun-Sang; Lee, Yong Seok;
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The importance of biological resources has been gradually increasing, and mollusks have been utilized as main fishery resources in terrestrial ecosystems. But little is known about genomic and transcriptional analysis in mollusks. This is the first report on the transcriptomic profile of Meretrix lusoria. In this study, we constructed cDNA library and determined 542 of distinct EST sequences composed of 284 singletons and 95 contigs. At first, we identified 180 of EST sequences that have significant hits on protein sequences of the exclusive Mollusks database through BLASTX program and 343 of EST sequences that have significant hits on NCBI NR database. We also found that 211 of putative sequences through local BLAST (blastx, E < e-10) search against KOG database were classified into 16 functional categories. Some kinds of immune response related genes encoding allograft inflammatory factor 1 (AIF-1), B-cell translocation gene 1 (BTG1), C-type lectin A, thioester-containing protein and 26S proteasome regulatory complex were identified. To determine phylogenetic relationship, we identified partial sequences of four genes (COX1, COX2, 12S rRNA and NADH dehydrogenase) that significantly matched with the mitochondrial genomes of 3 species-Ml (Meretrix lusoria), Mp (Meretrix petechialis) and Mm (Meretrix meretrix). As a result, we found that there was a little bit of a difference between sequences of Korean isolates and other known isolates. This study will be useful to develop breeding technology and might also be helpful to establish a classification system.
Meretrix lusoria;EST sequence;Molecular phylogenetic Study;
 Cited by
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Genes and Genomics, 2015. vol.37. 12, pp.1041-1049 crossref(new window)
Expressed sequence tag analysis and annotation of genetic information from the freshwater clam, Pisidium (Neopisidium) coreanum endemic to Korea, Genes & Genomics, 2015, 37, 12, 1041  crossref(new windwow)
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