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Variation of nitric oxide concentrations in response to shaking stress in the Manila clam Ruditapes philippinarum
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  • Journal title : The Korean Journal of Malacology
  • Volume 29, Issue 1,  2013, pp.1-6
  • Publisher : The Malacological Society of Korea
  • DOI : 10.9710/kjm.2013.29.1.1
 Title & Authors
Variation of nitric oxide concentrations in response to shaking stress in the Manila clam Ruditapes philippinarum
Park, Kyung-Il;
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This study aimed to evaluate the effects of shaking stress in the hemolymph of the Manila clam Ruditapes philippinarum by quantification of nitric oxide (NO) levels. The clams were divided into 3 groups as follows: clams placed in a plain container (control), clams injected with nitro-L-arginine methyl ester (NAME, an NO inhibitor), and clams in a container filled with nylon fiber at a density of . Subsequently, each group was placed in sea water and shaken at 100 rpm for 6 h. The concentration of NO was quantified by using DAF assay and Griess assay. Both the assays showed that while shaking significantly increased the NO concentration, the NO inhibitor reduced the NO concentration in the hemolymph of the clams tested. In addition, the nylon fiber, which was used as a filler, effectively prevented the increase in NO concentration. This result suggests that measurement of NO concentration is a useful tool for evaluation of physiological stress in marine bivalves. In addition, it should be considered that a filler is necessary when dredge fishing or the suspended clam culture method is developed.
nitric oxide;immune response;Ruditapes philippinarum;stress;clam culture;
 Cited by
고창 갯벌에서 중국산 및 충남산 바지락의 성장, 비만도 및 폐사 특성 비교,안현미;기훈종;정희도;이희중;한형균;박광재;송재희;

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