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Analysis of heat, cold or salinity stress-inducible genes in the Pacific abalone, Haliotis discus hannai, by suppression subtractive hybridization
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  • Journal title : The Korean Journal of Malacology
  • Volume 29, Issue 3,  2013, pp.181-187
  • Publisher : The Malacological Society of Korea
  • DOI : 10.9710/kjm.2013.29.3.181
 Title & Authors
Analysis of heat, cold or salinity stress-inducible genes in the Pacific abalone, Haliotis discus hannai, by suppression subtractive hybridization
Nam, Bo-Hye; Park, Eun-Mi; Kim, Young-Ok; Kim, Dong-Gyun; Jee, Young-Ju; Lee, Sang-Jun; An, Cheul Min;
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 Abstract
In order to investigate environmental stress inducible genes in abalone, we analyzed differentially expressed transcripts from a Pacific abalone, Haliotis discus hannai, after exposure to heat-, cold- or hyposalinity-shock by suppression subtractive hybridization (SSH) method. 1,074 unique sequences from SSH libraries were composed to 115 clusters and 986 singletons, the overall redundancy of the library was 16.3%. From the BLAST search, of the 1,316 ESTs, 998 ESTs (75.8%) were identified as known genes, but 318 clones (24.2%) did not match to any previously described genes. From the comparison results of ESTs pattern of three SSH cDNA libraries, the most abundant EST was different in each SSH library: small heat shock protein p26 (sHSP26) in heat-shock, trypsinogen 2 in cold-shock, and actin in hyposalinity SSH cDNA library. Based on sequence similarities, several response-to-stress genes such as heat shock proteins (HSPs) were identified commonly from the abalone SSH libraries. HSP70 gene was induced by environmental stress regardless of temperature-shock or salinity-stress, while the increase of sHSP26 mRNA expression was not detected in cold-shock but in heat-shock condition. These results suggest that the suppression subtractive hybridization method is an efficient way to isolate differentially expressed gene from the invertebrate environmental stress-response transcriptome.
 Keywords
Pacific abalone;Suppression subtractive hybridization;Expressed sequence tags;Response-to-stress gene;
 Language
English
 Cited by
1.
수온 및 염분 스트레스에 따 른 참담치, Mytilus coruscus에서 Hsp70 및 GST 유전자 발현에 대한 연구,김철원;강한승;

환경생물, 2015. vol.33. 4, pp.450-458 crossref(new window)
1.
Cloning and expression analysis of HSP70 gene from mangrove plant Kandelia obovata under cold stress, Ecotoxicology, 2015, 24, 7-8, 1677  crossref(new windwow)
 References
1.
Adams, M.D., Kelley, J.M., Gocayne, J.D., Dubnick, M., Polymeropoulos, M.H., Xiao, H., Merril, C.R., Wu, A., Olde, B., Moreno, R.F., Kerlayage, A.R., McCombie, W.R. and Venter, J.C. (1991) Complementary cDNA sequencing expressed sequence tags and human genome project. Science, 252; 1651-1656. crossref(new window)

2.
Cheng, W., Hsiao, I.S. and Chen, J.C. (2004a) Effect of ammonia on the immune response of Taiwan abalone Haliotis diversicolor supertexta and its susceptibility to Vibrio parahaemolyticus. Fish and Shellfish Immunology, 17:193-202. crossref(new window)

3.
Cheng, W., Hsiao, I.S. and Chen, J.C. (2004b) Effect of nitrite on immune response of Taiwan abalone Haliotis diversicolor supertexta and its susceptibility to Vibrio parahaemolyticus. Disease of Aquatic Organisms, 60:157-164. crossref(new window)

4.
Cheng, W., Hsiao, I.S., Hsu, C.H. and Chen, J.C. (2004c) Change in water temperature on the immune response of Taiwan abalone Haliotis diversicolor supertexta and its susceptibility to Vibrio parahaemolyticus. Fish and Shellfish Immunology, 17:235-243. crossref(new window)

5.
Cheng, W., Juang, F.M. and Chen, J.C. (2004d) The immune response of Taiwan abalone Haliotis diversicolor supertexta and its susceptibility to Vibrio parahaemolyticus at different salinity levels. Fish and Shellfish Immunology, 16: 295-306. crossref(new window)

6.
Cheng, W., Li, C.H. and Chen, J.C. (2004e) Effect of dissolved oxygen on the immune response of Haliotis diversicolor supertexta and its susceptibility to Vibrio parahaemolyticus. Aquaculture, 232: 103-115. crossref(new window)

7.
De Zoysa, M., Nikapitiya, C., Oh, C., Whang, I., Shin, H.J. and Lee, J. (2012) cDNA microarray analysis of disk abalone genes in gill and hemocytes after viral hemorrhagic septicemia virus (VHSV) challenge. Fish and Shellfish Immunology, 32: 1205-1215. crossref(new window)

8.
Diatchenko, L., Lau, Y.F., Campbell, A.P., Chenchik, A., Mogadam, F., Huang, B., Lukyanov, S., Lukyanov, K., Gurskaya, N., Syerdlov, E.D. and Siebert, P.D. (1996) Suppression subtractive hybridization: a method for generating differentially regulated or tissue-specific cDNA probes and libraries. Proceeding National Academic Science USA, 93: 6025-6030. crossref(new window)

9.
Hannon, C., Officer, R.A. and Le Dorven, J. (2013) Review of the technical challenges facing aquaculture of the European abalone Haliotis tuberculata in Ireland. Aquaculture International, 21: 243-245. crossref(new window)

10.
Hooper, C., Day, R., Slocombe, R., Handlinger, J. and Benkendorff, K. (2007) Stress and immune responses in abalone: limitations in current knowledge and investigative methods based on other models. Fish and Shellfish Immunology, 22: 363-379. crossref(new window)

11.
Lindquist, S. (1986) The heat-shock response. Annual Review Biochemistry, 55: 1151-1191. crossref(new window)

12.
Malham, S., Lacoste, A., Gelebart, F., Cueff, A. and Poulet, S. (2003) Evidence for a direct link between stress and immunity in the mollusk Haliotis tuberculata. Journal of Experimental Zoology, 295: 136-144.

13.
Martelle, L. and Tjeerdema, R.S. (2001) Combined effects of pentachorophenol and salinity stress on chemiluminescence activity in two species of abalone. Aquatic Toxicology, 51: 351-362. crossref(new window)

14.
Ottaviani, E. and Franceschi, C. (1997) The invertebrate phagocytic immunocyte: clues to a common evolution of immune and neuroendocrine systems. Immunology Today, 18: 169-173. crossref(new window)

15.
Parsons, J.D. (1995) Improved tools for DNA comparison and clustering. Comparative Applied Bioscience, 11: 603-613.