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Cryopreservation of Pacific Oyster, Crassostrea gigas Sperm
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  • Journal title : The Korean Journal of Malacology
  • Volume 29, Issue 3,  2013, pp.251-258
  • Publisher : The Malacological Society of Korea
  • DOI : 10.9710/kjm.2013.29.3.251
 Title & Authors
Cryopreservation of Pacific Oyster, Crassostrea gigas Sperm
Park, Mi Seon; Min, Byung Hwa; Park, Jung Jun; Lim, Hyun Jeong; Myeong, Jeong-In; Jeong, Min Hwan;
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This study aims to find out a suitable cryoprotective agent (CPA) for cryopreservation and its optimum concentration in order to conduct planned artificial seed production of Pacific oyster, Crassostrea gigas and to preserve superior sperm. For this, we tried to understand toxicity and the effect of cryopreservation by CPA type and concentrations first and then looked into cell damage of the sperm after thawing. Toxicity analysis on the sperm of Pacific oyster according to different CPA and immersion time shows that dimethyl sulfoxide (DMSO) comes first when it comes to survival rate and mobility followed by ethylene glycol (EG), glycerol and methanol. To identify the optimum CPA and its level, filtered seawater was used as a diluent before cryopreservation for 30 days. As a result, cryopreserved sperm of Pacific oyster with 15% of DMSO showed the highest survival rate and activation. Also, we observed the cryopreserved and thawed sperm with Scanning electron micrographs by CPAs and concentrations. Consequently, DSMO showed the lowest cell damage followed by EG, methanol, glycerol and the level was 15, 20, 10, 5% respectively. In a nutshell, it is proven that the optimum CPA and its level is 15% of DMSO.
Crassostrea gigas;sperm;cryopreservation;cryoprotective agents;
 Cited by
4배체 참굴(Crassostrea gigas)의 정자 동결보존,박미선;민병화;임현정;허영백;도용현;명정인;

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