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The change of the physiological response of the Crassostrea gigas exposed to PAHs
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  • Journal title : The Korean Journal of Malacology
  • Volume 30, Issue 3,  2014, pp.169-175
  • Publisher : The Malacological Society of Korea
  • DOI : 10.9710/kjm.2014.30.3.169
 Title & Authors
The change of the physiological response of the Crassostrea gigas exposed to PAHs
Choi, Eun Hee; Choi, Joong Ki; Lee, Won Young; Yoon, Ju Hyun; Shim, Na Young; Kim, Su Kyoung; Lim, Hyun Jeong;
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PAHs (Polycyclic Aromatic Hydrocarbons: PAHs) is the hydrophobic inorganic material composed of carbon and hydrogen that is easily adsorbed biological organisms in the ocean. Bivalves is the indicator of environment monitoring because of reflect growth, physiological response of bivalve followed their habitat environment. The aim of research is understand the change of oysters (Crassostrea gigas) physiological response under exposed PAHs concentration for control, 1, 10 and . We investigated induced immune change response for oyster hemocyte and effect of tissue RNA/DNA ratio for mantle, gill and adductor muscle individually. As a result of experiment change of immune response the oyster hemocyte when exposed PAHs showed that viability and adhesion is no significant difference (ANOVA test, p < 0.05). However phagocytosis decreased under the over of PAHs concentration and ROS increased with the increase of PAHs concentration. The change of RNA/DNA ratio is R/D ratio decreased with the increase of PAH concentration in adductor muscle. However gill and mantle showed no change of R/D ratio with PAHs concentration. The oysters when exposed inorganic pollutant that decreased of physiological condition and damaged protein synthesis of adductor muscle.
oyster;Crassostrea gigas;PAHs;hemocyte;R/D ratio;
 Cited by
Bushek, D., Heidenreich, M. and Porter, D. (2007) The effects of several common anthropogenic contaminants on proliferation of the parasitic oyster pathogen Perkinsus marinus. Marine Environmental Research, 64: 535-540. crossref(new window)

Clemmesen, C. (1994) The effect of food availability, age or size on the RNA and DNA content in individual measured herring larvae: laboratory calibration. Mar. Biol., 118: 377-382. crossref(new window)

Croxton, A.N., Wikfors, G.H. and Schulterbrandt-Gragg, R.D. (2012) Immunomodulation in eastern oysters, Crassostrea virginica, exposed to a PAH-contaminated, microphtobenthic diatom. Aquatic toxicology, 118-119: 27-36. crossref(new window)

Echeveste, P., Agusti, S. and Dachs, J. (2010) Cell size dependent toxicity thresholds of polycyclic aromatic hydrocarbons to natural and cultured phytoplankton populations. Environmental Pollution, 158: 299-307. crossref(new window)

Freitas Rebelo, M., Souza Figueiredo, E., Mariante, R.M., Nobrega, A., Barros, C.M., and Allodi, S. (2013) New Insights from the oyster Crassostrea rhizophorae on bivalve circulating hemocytes. PLOS ONE, 8: 1-6.

Gagnaire, B., Thomas-Guyon, H., Burgeot, T.h. and Renault, T. (2006) Pollutant effects on Pacific oyster, Crassostrea gigas (Thunberg), hemocytes : Screening of 23 molecules using flow cytometry. Cell Biology and Toxicology, 22: 1-14. crossref(new window)

Giron-Perez, M.I. (2010) Relationships between innate immunity in bivalve molluscs and environmental pollution. ISJ, 7: 149-156.

Hannam, M.L., Bamber, S.D., Moody, A.J., Galloway, T.S. and Jones, M.B. (2010) Immunotoxicity and oxidative stress in the Arctic scallop Chlamys islandica: Effects of acute oil exposure. Ecotoxicology and Environmental Safety, 73: 1440-1448. crossref(new window)

Hamoutene, D., Payne, J.F., Rahimtula, A. and Lee, K. (2002) Use of the comet assay to assess DNA damage in hemocytes and digestive gland cells of mussels and clams exposed to water contaminated with petroleum hydrocarbons. Marine Environmental Research, 54: 471-474. crossref(new window)

Hegaret, H., Wikfors, G.H. and Soudant, P. (2003a) Flow cytometric analysis of haemocytes from eastern oysters, Crassostrea virginica, subjected to a sudden temperature elevation I. Haemocyte type and morphology. J. Exp. Mar. Biol. Ecol., 293: 237-248. crossref(new window)

Hegaret, H., Wikfors, G.H. and Soudant, P. (2003b) Flow cytometric analysis of haemocytes from eastern oysters, Crassostrea virginica, subjected to a sudden temperature elevation II. Haemocyte functions: aggregation, viability, phagocytosis, and respiratory burst. J. Exp. Mar. Biol. Ecol., 293: 249-265. crossref(new window)

Hong, Y.W., Yuan, D.X., Lin, Q.M. and Yang, T.L. (2008) Accumulation and biodegradation of phenanthrene and fluoranthene by the algae enriched from a mangrove aquatic ecosystem. Marine Pollution Bulletin, 56: 1400-1405. crossref(new window)

Kim, P.H., Kim, M.J., Jo, M.R., Lee, D.S., Song, K.S., Byun, H.S., Cho, K.C., Park, K.J., Jun, J.C. and Yoon, H.D. (2010) Polycyclic Aromatic Hydrocarbons Hazard Assessment of shellfish due to Oil Spill Accidents. Kor. J. Fish Aquat. Sci., 43(3): 211-216. crossref(new window)

Kim, S.K., Rosenthal, H., Clemmesen, C., Park, K.Y., Kim, D.H., Choi, Y.S. and Seo, H.C. (2005) Various methods to determine the gonadal development and spawning season of the purplish Washington clam, Saxidomus purpuratus (Sowerby). J. Appl. Ichthyol., 21: 101-106. crossref(new window)

Kim, S., Lee, C., Kim, Y.D., Jo, Q., Lee, J.H., Park, M.W., Kim, J.W., and Gong, Y.G. (2009) Histological and biochemical analyses on reproductive cycle of Gomphina melanaegis (Bivalvia; Veneridae). J. Kor. Fish. Soc., 42(1): 41-47.

MIFAFF, (2010) Detailed survey and restoration after oil spill in Taean costal fisheries. MIFAFF: 498p.

Mitchelmore, C.L., Birmelin, C., Chipman, J.K. and Livingstone, D.R. (1998) Evidence for cytochrome P-450 catalysis and free radical involvement in the production of DNA strand breaks by benzo[a]pyrene and nitroaromatics in mussel (Mytilus edulis L.) digestive gland cells. Aquatic toxicology, 41: 193-212. crossref(new window)

Mirza, R., Mohammadi, M., Sohrab, A.D., Safahieh, A., Savari, A. and Hajeb, P. (2012) Polycyclic Aromatic Hydrocarbons in Seawater, Sediment, and Rock oyster Saccostrea cucullata from the Northern Part of the Persian Gulf (Bushehr Province). Water Air Soil Pollut, 223: 189-198. crossref(new window)

Nacci, D.E., Cayula, S., and Jackim, E. (1996) Detection of DNA damage in individual cells from marine organisms using the single cell gel assay. Aquatic toxicology, 35: 197-210. crossref(new window)

Newman M.C. (2010) Fundamentals of Ecotoxicology. CRC Press, Inc, Boca Raton.

Park, K.I., Choi, M.S. Park, S.W., Park, K.H., Choi, S.H. and Yoon J. M. (2009) Cellular Immune responses of the Manila Clam, Ruditapes philippinarum, Exposed to Cadmium Chloride. Korean J. Malacol., 25(2): 135-143.

Sami, S., Faisal, M. and Huggett, R.J. (1992) Alterations in cytometric characteristics of hemocytes from the American oyster Crassostrea virginica exposed to a polycyclic aromatic hydrocarbon (PAH) contaminated environment. Marine Biology, 113: 247-252.

Steinert, S.A. (1996) Contribution of Apoptosis to Observed DNA Damage in Mussel Cells. Marine Environmental Research, 42(1-4): 253-259. crossref(new window)