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Effect of five PAHs (2-methylnaphthalene, fluorene, dibenzothiophene, phenanthrene, and pyrene) on the embryonic development in the mussel, Mytilus galloprovincialis
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  • Journal title : The Korean Journal of Malacology
  • Volume 30, Issue 3,  2014, pp.177-187
  • Publisher : The Malacological Society of Korea
  • DOI : 10.9710/kjm.2014.30.3.177
 Title & Authors
Effect of five PAHs (2-methylnaphthalene, fluorene, dibenzothiophene, phenanthrene, and pyrene) on the embryonic development in the mussel, Mytilus galloprovincialis
Sung, Chan-Gyoung; Park, Pan-Soo; Lee, Jong-Hyeon; Lee, Chang-Hoon;
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Mussels have been commonly used in bioassay for quality assessments of environment. Moreover, several standard protocols for the developmental bioassay of bivalves have been proposed. In this study, the EC50 of polycyclic aromatic hydrocarbons (PAHs) was determined using mussel, Mytilus galloprovincialis embryonic developmental bioassay. To determine the sensitivity of M. galloprovincialis, their fertilized eggs were exposed to five PAHs (2-metylnaphthalene, fluorene, dibenzothiophene, phenanthrene, pyrene). The EC50 of 2-metylnaphthalene, fluorene, dibenzothiophene, phenanthrene, and pyrene were 232, 273, 67.9, 43.2, and , respectively. The overall sensitivity of M. galloprovincialis in the present developmental bioassay was similar to or more sensitive than that of other marine organisms commonly used in aquatic bioassays. The results of this study could be provide with fundamental data of setting standard for protection of marine life and or can use prediction the aquatic toxicity of PAHs.
Mytilus galloprovincialis;Embryonic development;bioassay;Sensitivity to PAHs;
 Cited by
Abernethy, S., Bobra, A., Shiu, W., Wells, P., Mackay, D. (1986) Acute lethal toxicity of hydrocarbons and chlorinated hydrocarbons to two planktonic crustsceans: The key roll of organism-water-partitioning. Aquatic Toxicology, 8: 163-174. crossref(new window)

Anderson, J.W., Neff, J.M., Cox, B.A., Tatem, H.E., Hightower, G.M. (1974) Characteristics of dispersions and water-soluble extracts of crude and refined oils and their toxicity to estuarine crustaceans and fish. Marine Biology, 27: 75-88. crossref(new window)

ASTM (American Society for Testing and Materials) (1994) Standard guide for conducting acute toxicity tests starting with embryos of four species of saltwater bivalve molluscs. ASTM E724-94. American Society for Testing and Materials, Philadelphia.

Barata, C., Navarro, J., Varo, I., Riva, M. Arun, S., Porte, C. (2005) Changes in antioxidant enzyme activities, fatty acid composition and lipid peroxidation in Daphnia magna during the aging process. Comparative Biochemistry and Physiology, B140: 81?90.

Battelle (2007) Sediment Toxicity of Petroleum Hydrocarbon Fractions. Massachusetts Department of Environmental Protection, Office of Research and Standards.

Beiras, R., Bellas, J., Fern?ndez, N., Lorenzo, J.I., Cobelo-Garcia, A. (2003a) Assessment of costal marine pollution in Galicia (NW Iberian Peninsula); metal concentration in seawater, sediment and mussels (Mytilus galloprovincialis) versus embryo-larval bioassays using Paracentrotus lividus and Ciona intestinalis. Marine Environmental Research, 56: 531-553. crossref(new window)

Beiras, R., Fern?ndez, N., Bellas, J., Besada, V., Gonz?lez-Quijano, A., Nunes, T. (2003b) Integrative assessment of marine pollution in Galician estuaries using sediment chemistry, mussel bioaccumulation, and embryo-larval toxicity bioassays. Chemosphere, 52: 1209-1224. crossref(new window)

Beiras, R. and Albentosa, M. (2004) Inhibition of embryo development of the commercial bivalves Ruditapes decussatus and Mytilus galloprovincialis by trace metals; Implication for the implementation of seawater quality criteria. Aquaculture, 230: 205-213. crossref(new window)

Beiras, R. and Bellas, J. (2008) Inhibition of embryo development of the Mytilus galloprovincialis marine mussel by organic pollutants; assessment of risk for its extensive culture in the Galician Rias. Aquaculture, 277(3-4): 208-212. crossref(new window)

Bellas J., Saco-Alvarez, L., Nieto, O., Beiras, R. (2008) Ecotoxicological evaluation of polycyclic aromatic hydrocarbons using marine invertebrate embryo?larval bioassays. Marine Pollution Bulletin, 57: 493-502. crossref(new window)

Boehm, P.D., Neff, J.M., Page, D.S. (2007) Assessment of polycyclic aromatic hydrocarbon exposure in the waters of Prince William Sound after the Exxon Valdez oil spill: 1989-2005. Marine Pollution Bulletin, (54): 339-367.

Boese, B., Lamberson, J., Swartz, R., Ozretich, R. (1997). Photoinduced toxicity of fluoranthene to seven marine benthic crustaceans. Archives of Environmental Contamination and Toxicology, 32: 389?393. crossref(new window)

Brooke, L.T. (1994) Acute Phenanthrene Toxicity to Daphnia magna U.S.EPA Contract No.68-C1-0034, Work Assignment No.2-14, to R.L.Spehar, U.S.EPA, Duluth, MN: 11 p.

Cachada, A., Pereira, R., Ferreira da Silva, E., Duarte, A.C. (2014) The prediction of PAHs bioavailability in soils using chemical methods: State of the art and future challenges. Science of The Total Environment, 472: 463-480. crossref(new window)

Castro-Jimenez, J., Berrojalbiz, N., Wollgast, J., Dachs J. (2012) Polycyclic aromatic hydrocarbons (PAHs) in the Mediterranean Sea: Atmospheric occurrence, deposition and decoupling with settling fluxes in the water column. Environmental Pollution, 166: 40-47. crossref(new window)

Call, D.J., Brooke, L.T., Harting, S.L., Poirier, S.H., McCauley, DJ. (1986) Toxicity of Phenanthrene to Several Freshwater Species. Rep. to Battelle Memorial Res. Inst., Columbus, OH, in Partial Fulfillment of Work Assignment No.45, Task Order No.4 of Subcontract No.F4114(8834)-411: 15 p.

Di Toro, D.M., McGrath, J.A., Hansen, D.J. (2000) Technical basis for narcotic chemicals and polycyclic aromatic hydrocarbon criteria I. Water and tissue. Environmental Toxicology and Chemistry, 19: 1951?1970. crossref(new window)

Donkin, P., Widdows, J., Evans, S.V., Worrall, C.M., Carr, M. (1989) Quantitative Structure-Activity Relationships for the Effect of Hydrophobic Organic Chemicals on Rate of Feeding by Mussels (Mytilus edulis). Aquatic Toxicology, 14(3): 277-294. crossref(new window)

Eastman D, Booth, G., Lee, M. (1984) Toxicity, accumulation, and elimination of polycyclic aromatic sulfur heterocycles in Daphnia magna. Archives of Environmental Contamination and Toxicology, 13: 105?111. crossref(new window)

Emery, V.L., Dillon, T.M. Jr. (1996) Chronic toxicity of phenanthrene to the marine polychaete worm, Nereis (Neanthes arenaceodentata). Bulletin of Environmental Contamination and Toxicology, 56: 265-270. crossref(new window)

Faria, M., Lopez, M.A., Fernandez-Sanjuan, M., Lacorte, S., Barata, C. (2010) Comparative toxicity of single and combined mixtures of selected pollutants among larval stages of the native freshwater mussels (Unio elongatulus) and the invasive zebra mussel (Dreissena polymorpha). Science of The Total Environment, 408(12): 2452-2458. crossref(new window)

Finger, S.E., Little, E.F., Henry, M.G., Fairchild, J.F., Boyle, T.P. (1985) Comparison of laboratory and field assessment of fluorene - Part I: Effects of fluorene on the survival, growth, reproduction, and behavior of aquatic organisms in laboratory tests. Pages 120-133 In: T.P. Boyle, Ed., Validation and Predictability of Laboratory Methods for Assessing the Fate and Effects of Contaminants in Aquatic Ecosystems. ASTM STP 865. American Society for Testing and Materials, Philadelphia, PA.

Geffard, O., His, E., Budzinski, H., Seaman, M., Garrigues, P. (2001) Qualite biologique de l'eau de merevalue in situ par le test embryo-larvaire de Crassostrea gigas et Mytilus galloprovincialis: Comptes Rendus de l'Academie des Sciences, Serie III. Sciences de la Vie, 324: 1149-1155.

Geiger, J.G. and Buikema, A.L. Jr. (1982) Hydrocarbons depress growth and reproduction of Daphnia pulex (Cladocera). Canadian Journal of Fisheries and Aquatic Sciences, 39: 830-836. crossref(new window)

Goldberg, E.D. and Bertine, K.K. (2000) Beyond the mussel watch - new directions for monitoring marine pollution. The Science of the Total Environment, 247(2-3): 165-174. crossref(new window)

Guitart, C., Frickers, P., Horrillo-Caraballo, J., Law, R.J., Readman, J.W. (2008) Characterization of sea surface chemical contamination after shipping accidents. Environmental Science and Technology, 42(7): 2275-2282. crossref(new window)

His, E., Seaman, M.N.L., Beiras, R. (1996) A simplification the bivalve embryogenesis and larval development bioassay method for water quality assessment. Water Research, 31(2): 351-355.

Hur, Y.B. and Hur, S.B. (2000) Development and growth of larvae of four bivalve species. Journal of Aquaculture, 13(2): 119-128. [in Korean]

International Tanker Owners Pollution Federation Ltd. (ITOPF) (2008) The environmental impact of the Hebei Sprit oil spill, Taean, South Korea. Available from : .

Jha, A.N., Cheung, V.V., Foulkes, M.E., Hill, S,J., Depledge, M.H. (2000) Detection of genotoxins in the marine environment: adoption and evaluation of an integrated approach using the embryo?larval stages of the marine mussel, Mytilus edulis. Mutation Research, 464(2): 213-228. crossref(new window)

Jung, K.H., Kim, J.K., Noh, J.H., Eun, J.W., Bae, H.J., Kim, M.G., Chang, T.G., Shen, Q., Kim, S.J., Kwon, S.H. Park, W.S., Lee, J.Y., Nam, S.W. (2013) Characteristic molecular signature for the early detection and prediction of polycyclic aromatic hydrocarbons in rat liver. Toxicology Letters, 216(1): 1-8. crossref(new window)

Kanaki, M., Nikolaou, A., Makri, C.A., Lekkas, D.F. (2007) The occurrence of priority PAHs, nonylphenol and octylphenol in inland and coastal waters of Central Greece and the Island of Lesvos. Desalination, 210(1-3): 16-23. crossref(new window)

Kennedy, C.J. (1990) Toxicokinetic study of chlorinated phenols and polycyclic aromatic hydrocarbons in rainbow trout (Oncorhynchus mykiss). Simon Fraser University.

Kennish, M.J. (1992) Ecology of Estuaries: Anthropogenic Effects. CRC Press, Boca Raton, FL.

Kurt, P.B. and Ozkoc, B. (2004) A survey to determine levels of chlorinated pesticides and PCBs in mussels and seawater from the Mid-Black Sea Coast of Turkey. Marine Pollution Bulletin, 48(11-12): 1076-1083. crossref(new window)

Kwon, J.H., Wuethrich, T., Mayer, P., Escher, B.I. (2009) Development of a dynamic delivery method for in vitro bioassays. Chemosphere. 76: 83?90. crossref(new window)

Latimer, J.S., Zheng, J. (2003) The sources, transport, and fate of PAHs in the marine environment. In: Douben, P.E. (Ed.), PAHs: An Ecotoxicological Perspective. John Wiley, London, pp. 9?33.

Lim, L., Wurl, O., Karuppiah, S., Obbard, J.P. (2007) Atmospheric wet deposition of PAHs to the sea-surface microlayer. Marine Pollution Bulletin, 54(8): 1212-1219. crossref(new window)

Meador, J.P. (2003) Bioaccumulation of PAHs in marine invertebrates. In: Douben, P.E. (Ed.), PAHs: An Ecotoxicological Perspective. John Wiley, London, pp. 147?171.

Millemann, R.E., Birge, W.J., Black, J.A., Cushman, R.M., Daniels, K.L., Franco, P.J., Giddings, J.M., McCarthy, J.F., Stewart, A.J. (1984) Comparative acute toxicity to aquatic organisms of components of coal-derived synthetic fuels. Transactions of the American Fisheries Society, 113: 74-85. crossref(new window)

Moreau, C.J., Klerks, P.L., Haas, C.N. (1999) Interaction between phenanthrene and zinc in their toxicity to the sheepshead minnow (Cyprinodon variegatus). Archives of Environmental Contamination and Toxicology, 37: 251-257. crossref(new window)

Nadella, S.R., Tellis, M., Diamond, R., Smith, S., Bianchini, A., Wood, C.M. (2013) Toxicity of lead and zinc to developing mussel and sea urchin embryos: Critical tissue residues and effects of dissolved organic matter and salinity Comparative Biochemistry and Physiology Part C: Toxicology and Pharmacology, 158(2): 72-83. crossref(new window)

Nicholson, S. and Szefer, P. (2003) Accumulation of mytilid mussel Perna viridis (Bivalvia: Mytilidae) from polluted and uncontaminated locations in Hong Kong coastal waters. Marine Pollution Bulletin, 46: 1035-1048. crossref(new window)

Pelletier, M.C., Burgess, R.M., Ho, K.T., Kuhn, A., McKinney, R.A., Ryba, S.A. (1997) Phototoxicity of Individual Polycyclic Aromatic Hydrocarbons and Petroleum to Marine Invertebrate Larvae and Juveniles. Environmental Toxicology and Chemistry, 16(10): 2190-2199. crossref(new window)

Qiu, Y.W., Zhang, G., Liu, G.Q., Guo, L.L., Li, X.D., Wai, O. (2009) Polycyclic aromatic hydrocarbons (PAHs) in the water column and sediment core of Deep Bay, South China. Estuarine, Coastal and Shelf Science, 83(1): 60-66. crossref(new window)

Rossi, S.S., Neff, J.M. (1978) Toxicity of polynuclear aromatic hydrocarbons to the polychaete Neanthes arenaceodentata. Marine Pollution Bulletin. 9(8): 220-223. crossref(new window)

Smith, S.B., Savino, J.F., Biouin, M.A. (1988) Acute toxicity to Daphnia pulex of six classes of chemical compounds potentially hazardous to Great Lakes biota. Journal of Great Lakes Research, 14: 394-404. crossref(new window)

Smolders, R., Bervoets, L., Blust, R. (2004) In situ and laboratory bioassays to evaluate the impact of effluent discharges on receiving aquatic ecosystems. Environmental Pollution, 132(2): 231-243. crossref(new window)

Song, M.K., Song, M., Choi, H.S., Kim, Y.J., Park, Y.K., Ryu, J.C. (2012) Identification of molecular signatures predicting the carcinogenicity of polycyclic aromatic hydrocarbons (PAHs). Toxicology Letters, 212(1):18-28. crossref(new window)

Sung, C.G., Kim, G.B., Seo, J.Y., Lee, C.H., Ryu, T.K., Han, G.M., Choi, J.W., Kim, Y.H. (2005) Optimal conditions of the embryonic development of mussel, Mytilus galloprovincialis. Korean Journal of Malacology, 21(1): 25-31. [in Korean]

Sung, C.G., Kim, G.B., Lee, C.H. (2006) Effect of heavy metals on embryonic development in the mussel, Mytilus galloprovincialis. Korean Journal of Malacology, 22(2): 167-173.

Trucco, R.G., Engelhardt, F.R., Stacey, B. (1983) Toxicity, accumulation and clearance of aromatic hydrocarbons in Daphnia pulex. Environmental Pollution (Series A). 31: 191-202. crossref(new window)

UNEP (2003) 잔류성유기오염물질의 국제적 규제를 위한 스톡홀름 협약, UNEP PRESS.

USEPA (1995) Short-Term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters to West Coast Marine and Estuarine Organisms. EPA/600/R-95-136.

Veith, G.D., Call, D.J., Brooke, L. (1983) Structure-toxicity relationships for the fathead minnow, Pimephales promelas: narcotic industrial chemicals. Canadian Journal of Fisheries and Aquatic Sciences, 40: 743?748. crossref(new window)

Walker, C.H., Hopkin, S.P., Sibly, R.M., Peakall, D.B. (2001) Principles of Ecotoxicology, second ed. Taylor & Francis, London.

Wang, Z., Hollebone, B.P., Fingas, M., Fieldhouse, B., Sigouin, L., Landriault, M., Smith, P., Noonan, J., Thouin, G. (2003) Characteristics of spilled oils, fuels, and petroleum products: Composition and properties of selected oils. EPA/600/R-03/072. U.S. Environmental Protection Agency, Washington, DC.

Wedderburn, J., McFadzen, I., Sanger, R.C., Beesley, A., Heath, C., Hornsby, M., Lowe, D. (2000) The field application of cellular and physiological biomarkers, in the mussel Mytilus edulis, in conjunction with early life stage bioassays and adult histopathology. Marine Pollution Bulletin, 40(3): 257-267. crossref(new window)

Weinstein, J.E., Polk, K.D. (2001) Phototoxicity of Anthracene and Pyrene to Glochidia of the Freshwater Mussel Utterbackia imbecillis. Environmental Toxicology and Chemistry, 20(9): 2021-2028. crossref(new window)