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Molecular Characterization and Expression Analysis of a Toll-like receptor 2/6 gene from Abalone (Haliotis discus hannai)
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  • Journal title : The Korean Journal of Malacology
  • Volume 31, Issue 3,  2015, pp.233-241
  • Publisher : The Malacological Society of Korea
  • DOI : 10.9710/kjm.2015.31.3.233
 Title & Authors
Molecular Characterization and Expression Analysis of a Toll-like receptor 2/6 gene from Abalone (Haliotis discus hannai)
Moon, Ji Young; Park, Eun Hee; Kong, Hee Jeong; Kim, Young-Ok; Kim, Dong-Gyun; An, Cheul Min; Nam, Bo-Hye;
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Toll-like receptors (TLRs) are a major pattern recognition receptor that recognize the structure of invading pathogen and play key roles by triggering immune response. In this study, we identified a sequence of TLR homolog and characterized at molecular level from the abalone (Haliotis discus hannai). Multiple alignments and phylogenetic analysis of abalone TLR protein belongs to the TLR 2/6. Expression level of abalone TLR 2/6 in the tissue was comparatively high in the mantle, gill, digestive duct, and hemocytes, but lowest in the muscle. Expression level of abalone TLR 2/6 mRNA in the mantle, gill, digestive duct, and hemocytes was 20-fold, 60-fold, 115-fold, 112-fold higher than in the muscle, respectively. Expression level of abalone TLR 2/6 mRNA in the mantle was steadily increased until 12 h and decreased post-infection with Vibrio parahemolyticus. While the expression level of abalone TLR 2/6 mRNA in the gill and hemocytes was drastically increased at 6 and 9 h post-infection with Vibrio parahemolyticus, respectively. These results suggest that abalone TLR 2/6 is conserved through evolution and may play roles similar to its mammalian counterparts.
Haliotis discus hannai;pattern recognition receptor;Toll-like receptor;Vibrio parahemolyticus;
 Cited by
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