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Whole Transcriptomic Analysis of Bacillus anthracis during Hydrogen Peroxide Decontamination
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 Title & Authors
Whole Transcriptomic Analysis of Bacillus anthracis during Hydrogen Peroxide Decontamination
Kim, Sang Hoon; Kim, Se Kye; Jung, Kyoung Hwa; Yoon, Sung Nyo; Kim, Yun Ki; Kim, Min Cheol; Ryu, Sam Gon; Lee, Hae Wan; Chai, Young Gyu;
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Decontamination of biological agents utilizes hydrogen peroxide() for its effectiveness and safeness. Bacillus anthracis is a major target for decontamination. To assess the effect of on B. anthracis and identify biomarkers for decontamination, whole transcriptomic profiling of -treated B. anthracis was performed. Here we identified deregulation in stress response genes, transcription factors and cellular homeostasis genes. We also found that expression of antisense RNAs increased in B. anthracis during decontamination. We postulate that B. anthracis prioritizes survival and adaptation in response to treatment by changing its gene expression pattern.
Bacillus anthracis;Transcrptome;Hydrogen Peroxide;Decontamination;
 Cited by
Z. Dai, J. C. Sirard, M. Mock and T. M. Koehler, "The atxA Gene Product Activates Transcription of the Anthrax Toxin Genes and Is Essential for Virulence," Mol Microbiol, Vol. 16, No. 6, pp. 1171-1181, 1995. crossref(new window)

M. Mock and A. Fouet, "Anthrax," Annu Rev Microbiol, Vol. 55, pp. 647-671, 2001. crossref(new window)

L. Bailli, S. Hibbs, P. Tsai, G. L. Cao and G. M. Rosen, "Role of Superoxide in the Germination of Bacillus anthracis Endospores," FEMS Microbiol Lett, Vol. 245, pp. 33-38, 2005. crossref(new window)

N. A. Klapes and D. Vesley, "Vapor-Phase Hydrogen Peroxide as a Surface Decontaminant and Sterilant", Appl Environ Microbiol, Vol. 56, pp. 503-506, 1990.

T. L. Buhr, A. A. Young, Z. A. Minter, C. M. Wells, D. C. McPherson, C. L. Hooban, C. A. Johnson, E. J. Prokop, and J. R. Crigler, "Test Method Development to Evaluate Hot, Humid Air Decontamination of Materials Contaminated with Bacillus anthracis Sterne and Bacillus thuringiensis Al Hakam Spores", J Appl Microbiol, Vol. 113, pp. 1037-1051, 2012. crossref(new window)

J. V. Rogers, C. L. Sabourin, Y. W. Choi, W. R. Richter, D. C. Rudnicki and K. B. Riggs, "Decontamination Assessment of Bacillus anthracis, Bacillus subtilis, and Geobacillus stearothermophilus Spores on Indoor Surfaces Using a Hydrogen Peroxide Gas Generator", J Appl Microbiol, Vol. 99, pp. 739-748, 2005. crossref(new window)

A. C. Pinto, H. P. Melo-Barbosa, A. Miyoshi, A. Silva and V. Azevedo, "Application of RNA-seq to Reveal the Transcript Profile in Bacteria," Genet Mol Res, Vol. 10, No. 3, pp. 1707-1718, 2011. crossref(new window)

R. McClure, D. Balasubramanian, Y. Sun, M. Bobrovskyy, P. Sumby, C. A. Genco, C. K. Vanderpool and B. Tjaden, "Computational Analysis of Bacterial RNA-Seq Data," Nucleic Acids Res, Vol. 41, pp. 140, 2013. crossref(new window)

W. Huang da, B. T. Sherman and R. A. Lempicki, "Systematic and Integrative Analysis of Large Gene Lists Using DAVID Bioinformatics Resources," Nat Protoc, Vol. 4, pp. 44-57, 2009.

S. H. Kim, S. K. Kim, K. H. Jung, Y. K. Kim, H. C. Hwang, G. R. Sam and Y. G. Chai, "Proteomic Analysis of the Oxidative Stress Response Induced by Low-Dose Hydrogen Peroxide in Bacillus anthracis," J Microbiol Biotechnol, Vol. 23, No. 6, pp. 750-758, 2013. crossref(new window)

E. R. Schafer, M. J. Oneal, M. L. Madsen, F. C. Minion, "Global Transcriptional Analysis Of Mycoplasma Hyopneumoniae Following Exposure to Hydrogen Peroxide" Microbiology, Vol. 153, pp. 3785-3790, 2007. crossref(new window)

S. Altuvia, D. Weinstein-Fischer, A. Zhang, L. Postow and G. Storz, "A Small, Stable RNA Induced by Oxidative Stress : Role as a Pleiotropic Regulator and Antimutator," Cell, Vol. 90, No. 1, pp. 43-53, 1997. crossref(new window)

N. Delihas and S. Forst, "MicF : an Antisense RNA Gene Involved in Response of Escherichia coli to Global Stress Factors," J Mol Biol, Vol. 313, No. 1, pp. 1-12, 2001. crossref(new window)