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Effects of Sagan-tang and individual herbs on COPD Mice Model
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  • Journal title : Herbal Formula Science
  • Volume 23, Issue 2,  2015, pp.171-187
  • Publisher : The Korean Journal of Oriental Medical Prescription
  • DOI : 10.14374/HFS.2015.23.2.171
 Title & Authors
Effects of Sagan-tang and individual herbs on COPD Mice Model
Han, Jong-Min; Yang, Won-Kyung; Kim, Seung-Hyeong; Park, Yang-Chun;
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Objective This study aimed to evaluate the effects of Sagan-tang (SGT) on COPD mouse model. Methods The study was carried out by two ways (in vitro, in vivo). In vitro RAW264.7 cells (mouse macrophage) were used and analysed by flow cytometry, ELISA, Western blot. In vivo LPS and CSS challenged mice were used and its BALF had been analysed by cytospin image, FACS, ELISA, lung tissue by real-time PCR. Results In vitro, SGT maintained 80-100% rate of viablilty on 10 ~ 500 ㎍/㎖ concentration. In ELISA analysis with RAW264.7 cells, SGT significantly decreased NO over 30 ㎍/㎖. In flow cytometry, SGT 100 ㎍/㎖ dosage group displayed a tendency for decrease ROS. In Western blot analysis, SGT 100 ㎍/㎖ dosage group decreased NF-κB. In ELISA analysis, SGT significantly decreased TNF-α, IL-6 over 200 ㎍/㎖. In vivo SGT 200 ㎎/㎏ dosage group, application of SGT significantly decreased increase of neutrophils, TNF-α, IL-6 in BALF, muc5AC, TGF-β, TNF-α, expression of mRNA in lung tissue and histological lung injury. Conclusion This Study suggests usability of SGT for COPD patients by controlling lung tissue injury.
Sagan-tang (shègān-tāng);chronic obstructive pulmonary disease;cigarette smoke solution;
 Cited by
Effects of Gwaruhaengryeon-hwan on COPD and Particulate Matter Induced Lung Injury on a Mouse Model, The Journal of Internal Korean Medicine, 2017, 38, 3, 353  crossref(new windwow)
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