JOURNAL BROWSE
Search
Advanced SearchSearch Tips
Molecular adaptation of the CREB-Binding Protein for aquatic living in cetaceans
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
Molecular adaptation of the CREB-Binding Protein for aquatic living in cetaceans
Jeong, Jae-Yeon; Chung, Ok Sung; Ko, Young-Joon; Lee, Kyeong Won; Cho, Yun Sung; Bhak, Jong; Yim, Hyung-Soon; Lee, Jung-Hyun;
  PDF(new window)
 Abstract
Cetaceans (whales, dolphins, and porpoises) are aquatic mammals that experienced drastic changes during the transition from terrestrial to aquatic environment. Morphological changes include streamlined body, alterations in the face, transformation of the forelimbs into flippers, disappearance of the hindlimbs and the acquisition of flukes on the tail. For a prolonged diving, cetaceans acquired hypoxia-resistance by developing various anatomical and physiological changes. However, molecular mechanisms underlying these adaptations are still limited. CREB-binding protein (CREBBP) is a transcriptional co-activator critical for embryonic development, growth control, metabolic homeostasis and responses to hypoxia. Natural selection analysis of five cetacean CREBBPs compared with those from 15 terrestrial relatives revealed strong purifying selection, supporting the importance of its role in mammals. However, prediction for amino acid changes that elicit functional difference of CREBBP identified three cetacean specific changes localized within a region required for interaction with SRCAP and in proximal regions to KIX domain of CREBBP. Mutations in CREBBP or SRCAP are known to cause craniofacial and skeletal defects in human, and KIX domain of CREBBP serves as a docking site for transcription factors including c-Myb, an essential regulator of haematopoiesis. In these respects, our study provides interesting insights into the functional adaptation of cetacean CREBBP for aquatic lifestyle.
 Keywords
cetacean;CREB-binding protein;aquatic adaptation;craniofacial;haematopoiesis;
 Language
English
 Cited by
 References
1.
Thewissen, J. G., Cooper, L. N., Clementz, M. T., Bajpai, S., and Tiwari, B. N. 2007. Whales originated from aquatic artiodactyls in the Eocene epoch of India. Nature. 450, 1190-1194. crossref(new window)

2.
Uhen, M. D. 2007. Evolution of marine mammals: back to the sea after 300 million years. Anat. Rec. (Hoboken). 290, 514-522. crossref(new window)

3.
Reidenberg, J. S. 2007. Anatomical adaptations of aquatic mammals. Anat. Rec. (Hoboken). 290, 507-513. crossref(new window)

4.
Kooyman, G. L. 2009. Diving physiology. Encyclopedia of marine mammals. 2nd Ed. 327-332.

5.
Blobel, G. A. 2002. CBP and p300: versatile coregulators with important roles in hematopoietic gene expression. J. Leukoc. Biol. 71, 545-556.

6.
Oike Y., Takakura, N., Hata, A. et al., 1999. Mice homozygous for a truncated form of CREB-binding protein exhibit defects in hematopoiesis and vasculo-angiogenesis. Blood. 93, 2772-2779.

7.
Petrij, F., Giles, R. H., Dauwerse, H. G. et al. 1995. Rubinstein-Taybi syndrome caused by mutations in the transcriptional co-activator CBP. Nature. 376, 348-351. crossref(new window)

8.
Yim, H. S., Cho, Y. S., Guang, X. et al., 2014. Minke whale genome and aquatic adaptation in cetaceans. Nat. Genet. 46, 88-92.

9.
Loytynoja, A. and Goldman, N. 2005. An algorithm for progressive multiple alignment of sequences with insertions. Proc. Natl. Acad. Sci. 30, 10557-10562.

10.
Letunic, I. and Bork, P. 2006. Interactive Tree Of Life (iTOL): an online tool for phylogenetic tree display and annotation. Bioinformatics 23, 127-128.

11.
Liang, L., Shen, Y. Y., Pan, X. W. et al. 2013. Adaptive Evolution of the Hox Gene Family for Development in Bats and Dolphins. PLoS ONE 8(6), e65944.

12.
Yang, Z. 2007. PAML 4: phylogenetic analysis by maximum likelihood. Mol. Biol. Evol. 24, 1586-1591. crossref(new window)

13.
Zhang, J., Nielsen. R. and Yang, Z. 2005. Evaluation of an improved branch-site likelihood method for detecting positive selection at the molecular level. Mol. Biol. Evol. 22, 2472-2479. crossref(new window)

14.
Sievers, F. and Higgins, D. G. 2014. Clustal Omega, accurate alignment of very large numbers of sequences. Methods Mol. Biol. 1079, 105-116. crossref(new window)

15.
Adzhubei, I. A., Schmidt, S., Peshkin, L. et al. 2010. A method and server for predicting damaging missense mutations. Nat. Methods 7, 248-249. crossref(new window)

16.
Yang, Z. and Bielawski, J. P. 2000. Statistical methods for detecting molecular adaptation. Trends Ecol. Evol. 15, 496-503. crossref(new window)

17.
Hood, R. L., Lines, M. A., Nikkel, S. M. et al. 2012. Mutations in SRCAP, encoding SNF2-related CREBBP activator protein, cause Floating-Harbor syndrome. Am. J. Hum. Genet. 90, 308-313. crossref(new window)

18.
Thakur, J.K., Yadav, A. and Yadav, G. 2014. Molecular recognition by the KIX domain and its role in gene regulation. Nucleic Acids Res. 42, 2112-2125. crossref(new window)

19.
Mucenski, M. L., McLain, K., Kier, A.B. et al. 1991. A functional c-myb gene is required for normal murine fetal hepatic hematopoiesis. Cell. 65, 677-689. crossref(new window)

20.
Yu, B. D., Hess, J. L., Horning, S. E. et al. 1995. Altered Hox expression and segmental identity in Mll-mutant mice. Nature. 378, 505-508. crossref(new window)

21.
Semenza, G. L. 2012. Hypoxia-inducible factors in physiology and medicine. Cell. 148, 399-408. crossref(new window)