JOURNAL BROWSE
Search
Advanced SearchSearch Tips
An ultrastructural study of the cuticle in the byssus of marine mussel (Mytilus coruscus)
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
An ultrastructural study of the cuticle in the byssus of marine mussel (Mytilus coruscus)
Kim, Sangsik; Choi, Seung Hwan; Yoon, Sung Jin; Hwang, Dong Soo;
  PDF(new window)
 Abstract
Mussel byssus is a bundle of threads used to attach mussels to wet substrates. Recently, a thin cuticle layer on the byssus has attracted public attentions due to its remarkable toughness - stiff as epoxy resin and extensible as rubber. Here, we observed ultrastructure of the cuticle layer in a far eastern mussel (Mytilus coruscus) to understand underlying mechanisms for the mechanical properties. The cuticle layer observed by TEM was composed of submicron-sized granular inclusions in a continuous matrix phase. In addition, ultrastructural study in the presence of tertiary amine (Tetraethylammonium, TEA) showed an evidence that the cuticle is stabilized by cation- interaction.
 Keywords
Byssal coating;cuticle;cation- interaction;
 Language
Korean
 Cited by
 References
1.
Lee BP, Messersmith PB, Israelachvili JN, Waite JH. 2011. Mussel-inspired adhesives and coatings. Annu. Rev. Mater. Res. 41, 99-132. crossref(new window)

2.
H.J.Cha, D.S.Hwang and S.Lim. 2008. Development of bioadhesives from marine mussels. Biotechnol. J. 3, 631-638. crossref(new window)

3.
H.G.Silverman and F.F.Roberto. 2007. Understanding Marine Mussel Adhesion. Mar.Biotechnol. 9, 661-681. crossref(new window)

4.
C.P.Barnes, S.A.Sell, E.D.Boland, D.G.Simpson and G.L.Bowlin. 2007. Designing the next generation of tissue engineering scaffolds. Adv. Drug. Deliv. Rev. 59, 1413-1433. crossref(new window)

5.
Holten-Andersen N, Thomas E.Mates et al. 2009. Metals and the Integrity of a Biological Coating: The Cuticle of Mussel Byssus. Langmuir. 25, 3323-3326. crossref(new window)

6.
Holten-Andersen N and J.H.Waite. 2008. Mussel-designed protective coatings for compliant substrates. J Dent Res. 87, 700-709.

7.
Holten-Andersen N, Fantner GE, Hohlbauch S, Waite JH, Zok FW. 2007. Protective coatings on extensible biofibres. nature materials. 6, 669-672. crossref(new window)

8.
R.MacKinnon, G.Yellen. 1990. Mutations affecting TEA blockade and ion permeation in voltage-activated K+ channels. Science, 250, 276-279. crossref(new window)

9.
C.A.Ahern, A.L.Eastwood et al. 2006. A Cation-$\pi$ Interaction between Extracellular TEA and an Aromatic Residue in Potassium Channels. J. Gen. Physiol. 128, 649-657. crossref(new window)

10.
L.Heginbo-tham, R.MacKinnon. 1992. The aromatic binding site for tetraethylammonium ion on potassium channels. Neuron 8, 483-491. crossref(new window)

11.
S.A.Pless, J.D.Galpin et al. 2011, A novel mechanism for fine-tuning open state stability in a voltage-gated potassium channel. Nat.Commun. 2, 351 crossref(new window)

12.
J.H.Waite, 1983. vidence for a repeating 3,4-dihydroxyphenylalanine- and hydroxyproline - containing decapeptide in the adhesive protein of the mussel, Mytilus edulis L. J.Biol.Chem. 258, 2911-2915.

13.
S.W.Taylor, D.B.Chase et al. 1996. Ferric Ion Complexes of a DOPA-Containing Adhesive Protein from Mytilus edulis, Inorg.Chem. 35, 7572-7577. crossref(new window)

14.
Qi Lin, Delphine Gourdon et al. 2007. Adhesion mechanisms of the mussel foot proteins mfp-1 and mfp-3. Natl.Acad.Sci.U.S.A. 104, 3782-3786. crossref(new window)

15.
J.H.Waite, 1986. Mussel glue from Mytilus californianus Conrad: a comparative study. J. Camp. Physiol. B 156, 491-496. crossref(new window)

16.
J.H.Waite, J.H., et al. 1989. The glue protein of ribbed mussels (Geuhensia demissa): a natural adhesive with some features of collagen. J. Comp. Physiol. B 159, 517-525. crossref(new window)

17.
J.H.Waite, L.M Rzepecki. 1991. alpha, beta-Dehydro-3, 4-dihydroxyphenylalanine derivatives: rate and mechanism of formation. Arch. Physiol. Biochem. 285, 27-36 crossref(new window)

18.
Koji Inoue, Yasuhiro Takeuchi et al. 1996. Adhesive Protein cDNA Sequence of the Mussel Mytilus coruscus and Its Evolutionary Implications. J Mol Evol 43, 348-356. crossref(new window)

19.
S.W.Taylor, D.B.Chase et al. 1996. Ferric ion complexes of a DOPA-containing adhesive protein from Mytilusedulis. Inorg.Chem. 35, 7572-7577. crossref(new window)

20.
M.J.Harrington, A.Masic et al. 2010. Iron-Clad Fibers: A Metal-Based Biological Strategy for Hard Flexible Coatings. Science 328, 216-220. crossref(new window)

21.
H.Zeng, D.S.Hwang et al., 2010. Strong Reversible $Fe^{3+}$ -mediated Bridging between Dopa-Containing Protein Films in Water. Natl.Acad.Sci.U.S.A. 107, 12850-12853 crossref(new window)

22.
D.S.Hwang, A.Masic et al. 2013. Marine Hydroid Perisarc: A chitin- and melanin-reinforced composite with DOP A-iron (III) complexes. Acta Biomater. 9, 8110-8117 crossref(new window)

23.
J.C.Ma, D.A.Dougherty, 1997. The Cation-$\pi$ Interaction. Chem. Rev. 97,1303-1324. crossref(new window)

24.
Dougherty DA. 1996. Cation-$\pi$ interactions in chemistry and biology: A new view of benzene, Phe, Tyr, and Trp. Science, 271, 163-168. crossref(new window)

25.
G.Waksman, D.Cowburn et al., 1992. Crystal structure of the phosphotyrosine recognition domain $SH_2$ of v-src complexed with tyrosine-phosphorylated peptides. Nature 358, 646-665. crossref(new window)