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Characterization of α-D-manosidase activity from Bacillus safensis MA-01
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 Title & Authors
Characterization of α-D-manosidase activity from Bacillus safensis MA-01
Lee, Bo Mi; Kim, Joo Won; Park, Jae Kweon;
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An extracellular alkaline -D-mannosidase produced by a strain named as MA-01 was produced and its preliminary enzyme activity was characterized. Upon determining the 16S rDNA sequence and its homology search, the strain was identified to be one of species of the Bacillus safensis. Localization of enzyme was elucidated that -D-mannosidase can be found in culture medium as an extracellular enzyme. In addition, partial enzyme activity of 63% compared with the extracellular enzyme activity was observed in membrane protein. The optimal pH and temperature of the -D-mannosidase were pH 7.5 and , respectively. The and values of the -D-mannosidase in crude enzyme toward p-nitrophenyl--D-mannopyranoside were determined to be and of protein, respectively. To the best of our knowledge, this is the first report described the alkaline -D-mannosidase from the family of B. safensis.
Bacillus safensis;--D-Mannosidase;16S rDNA sequence;Membrane protein;p-nitrophenyl--D-mannopyranoside;
 Cited by
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