JOURNAL BROWSE
Search
Advanced SearchSearch Tips
Biodegradation Characteristics of Monochlorophenols by Wood Rot Fungi
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
Biodegradation Characteristics of Monochlorophenols by Wood Rot Fungi
Choi, In-Gyu; Lee, Jae-Won; Choi, Don-Ha;
  PDF(new window)
 Abstract
Biodegradation of monochlorophenols by wood rot fungi such as Daldina concentrica, Trametes versicolor and Pleurotus ostreatus was evaluated by determining their resistance or toxic test and biodegradability. The metabolites of monochlorophenols were also analyzed. Among the three fungi, T. versicolor was the most resistant to 200 ppm of 2-, 3- and 4-chlorophenols, and did not show any inhibitory mycellium growth. But D. concentrica had a little inhibition effect at more than 100 ppm of 3- or 4-chlorophenol. Control cultures of P. ostreatus took even 14 days far the completion of mycellium growth, but the hyphal growth was improved when 2- or 3-chlorophenol were added to the culture. In biodegradation analysis, P. ostreatus showed the highest degradation of 2- or 3-chlorophenol, while T. versicolor was the most effective in 4-chlorophenol. D. concentrica and P. ostreatus slowly degraded 4-chlorophenol. However, T. versicolor had similar degradation capability in the three monochlorophenols, suggesting that the biode- gradation nude is dependent on the fungi as well as the type of monochlorophenol. Several metabolites such as 1,3,5-trihydroxyl benzene, 1-ethyl-1-hydroxyl pentane, 2-propenoicacid, methylmalonic acid and 2-methyl-4-keto-pentan-2-ol were found as products of primary oxidation of 2-, 3- and 4-chlorophenols by intact fungal cultures. fatty acids including tetradecanoic, heptadecanoic and octadecanoic acids were also detected The order of increase of mycellium weight during incubation were P. ostreatus > T. versicolor > D. concentrica. The pH in the culture was not constantly changed depending on incubation days, but the mycellium weight was slightly increased, indicating that the biodegradation of monochlorophenol might have low relationship with the mycellium growth Laccase activities of T. versicolor and P. ostreatus were continuously increased depending on the incubation days, suggesting that the ligninolytic enzyme activity play an important role in the biodegradation of monochlorophenol.
 Keywords
monochlorophenol;Daldina concentrica;Trametes versicolor;Pleurotus ostreatus;biodegradation;
 Language
Korean
 Cited by
1.
Dibutyl phthalate biodegradation by the white rot fungus,Polyporus brumalis, Biotechnology and Bioengineering, 2007, 97, 6, 1516  crossref(new windwow)
 References
1.
Rochkind-Dubinsky, M. L., Sayler, G. S. and Blackburn, J. W. (1987) Microbiological decomposition of chlorinated aromatic compounds, Marcel Dekker Inc., NY and Basel, p.73-107

2.
Sierra-Alvarez, R., Field, J. A., Kortekaas, S. and Lettinga, G. (1994) Overview of the anaerobic toxicity caused by organic forest industry wastewater pollutants, Wat. Sci. Technol. 29, 353-363

3.
Huynh, V., Chang, H., and Joyce, T. (1985) Dechlorination of chloroorganics by a whiterot fungi, Tappi Journal 68(7), 98-102

4.
Krumm, M. L., and Boyd, S. A. (1988) Reductive dechlorination of chlorinated phenols in anaerobic upflow bioreactors, Wat. Res. 22, 171-177 crossref(new window)

5.
Roy-Arcand, L. and Archibald, F. S. (1991) Direct dechlorination of chlorphenolic compounds by laccase from Trametes(Coriolus) versicolor, Enzyme Microb. Technol. 13, 194-203

6.
Kubatova, A., Erbanova, P., Eichlerova, I., Homolka, L., Nerud, F. and Sasek, V.(2000) PCB congener selective biodegradation by the white rot fungus Pleurotus ostreatus in contaminated soil, Chemosphere. 43, 207-215 crossref(new window)

7.
Ruttimann, J. C. and Lamar, R T. (1996) Polymerization of pentachlorophenol and ferulic acid by fungal extracellular lignindegrading enzymes, Appl. Environ. Microbiol. 62, 3890-3893

8.
Leonitevsky, A. A., Myasoedova, N. M., Baskunov, B. P., Evans, C. S. and Golovleva, L. A. (2000) Transformation of 2,4,6-trichlorophenol by the white rot fungi Panus tigrinus and Coriolus versicolor, Biodegradation. 11, 331-340 crossref(new window)

9.
尹藤弘道, 本田与一, 渡辺降可, 桑原正章 (1998) 食用擔子 菌を用いたバイオレメデ イエ一シヨンに關する硏究 -ヒラタケによる鹽素化フェノ一ルの分解, 日本木村學會大會硏究發表要旨集, 501

10.
Katayame, H. (1994) Biodegradation of phenol and monochlorophenols by yeast Rhodotorula glutinis, Wat. Sci. Tech. 30, 59-66

11.
Chang, B. V., Wu, W. B. and Yuan, S. Y. (1997) Biodegradation of benzene, toluene, and other aromatic compounds by Pseudomonas sp. D8, Chemosphere. 35, 2807-2815 crossref(new window)

12.
Mileski, G. J., Bumpus, J. A., Jurek, M. A. and Aust, S. D. (1988) Biodegradation of pentachlorophenol by the white rot fungus Phanerochaete chrysosporium, Appl. Environ. Microbiol. 59, 1779-1785

13.
Valli, K. and Gold, M. (1991) Degradation of 2,4-dichlorophenol by the lignindegrading gungus Phanerochaete chrysosporium, J. Bacterial. 173, 345-352

14.
Alleman, B. C., Logan, B. and Gibertson, R. L. (1992)Toxicity of pentachlorophenol to six species of white rot fungi as a function of chemical dose, Appl. Environ. Microbiol. 58, 4048-4050

15.
Reddy, G. V. B., Gelpke, M. D. S. and Gold, M. H. (1998) Degradation of 2,4,6-trichlorophenol by Phanerochaete chrysosporium : involvement of recductive dechlorination, J. Bacteriol. 180, 5159-5164

16.
Armenante, P. M., Pal, N. and Lewandowski, G. (1994) Role of mycelium and extracellular protein in the biodegradation of 2,4,6-trichlorophenol by Phanerochaete chrysosporium, Appl. Environ. Microbiol. 60, 1711-1718

17.
Shuttleworth, K. L. and Bollag, J. M. (1986) Soluble and immobilized laccase as catalysts for the transformation of substituted phenols, Enzyme Microb. Technol. 8, 171-177 crossref(new window)

18.
Ruggiero, P., Sarkar, J. M. and Bollag, J. M. (1989) Detoxification of 2,4,-dichlorophenol by a laccase immobilized on soil or clay, Soil Science. 147, 361-370 crossref(new window)

19.
Iimura, Y., Hartikainen, P. and Tatsumi, K. (1996) Dechlorination of tetrachloroguaiacol by laccase of white rot basidiomycete Coriolus versicolor, Appl. Microbiol. Biotechrol. 45, 434-439 crossref(new window)

20.
Tien, M., and Kirk T. K. (1984) Ligninde-grading enzyme from Phanerochaete chrysosporium: purification, characterization and catalytic properties of an unique $H_2O_2$-requiring oxygenase, Proc. Natl. Acad. Sci. USA 81, 2280-2284 crossref(new window)

21.
최인규, 안세희 (1998) 목질분해균에 의한 Pentachloro-phenol의 미생물분해, 한국목재공학회, 26(3), 53-62

22.
최인규 (1999) Biodegradation of Chlorinated Phenols, Pentachlorophenol and 4,5,6-Trichloroguaiacol, by LigninDegrading Basidiomycetes, Grammothele fuligo, and Phanerochaete crassa, 산림과학논문집, 62, 53-66