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[ Sr2+ ] Stimulation of α-amylase and RNAse in Wheat Aleurone Layer
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[ Sr2+ ] Stimulation of α-amylase and RNAse in Wheat Aleurone Layer
Kim, Tae-Wan;
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To measure an effects of strontium on secretion of -amylase and RNase, wheat aleurone layers were isolated after the pre-incubation in a solution with or without 10 mM or for 3 days at in the dark under aseptic conditions. The secretion of -amylase reached a maximum at 72 h after incubation. induced more effectively secretion of -amylase than . The -amylase secretions by or ware about to fold higher than it without divalent ions, When aleurone layers were incubated without divalent ions, however, the -amylase was remarkably retained in the tissues. Total -amylase synthesis (ie. tissues + media) was slightly lowered by 10mM addition. It seemed that the RNase secretion begins at 18 h after incubation. This meaned that the RNase secretion may process slower than -amylasee secretion. effect on RNase secretion is stronger than unlikely to -amylase. The secretion process is likely to be suddenly induced between 72 hand 96 h. These results suggested that the secretion was enhanced after the accumulation in aleurone layers.
aleulone layer;-amylase;RNase;secretion;strontium;wheat;
 Cited by
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