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The Effect of Mixed Amino Acids on Nitrate Uptake and Nitrate Assimilation in Leafy Radish
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 Title & Authors
The Effect of Mixed Amino Acids on Nitrate Uptake and Nitrate Assimilation in Leafy Radish
Liu, Xing-Quan; Kim, Young-Sun; Lee, Kyu-Seung;
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 Abstract
The objective of the present work was to determine the corresponding uptake and assimilation of in roots and shoots of leafy radish by applying of mixed amino acids (MAA). The amino acids used in this experiment were alanine (Ala), , aspartic acid (Asp), asparagines (Asn), glutamic acid (Glu), glutamine (Gln), and glycine (Gly). Leafy radish was grown by conventional fertilization with macro- and micronutrients under controlled conditions. The 15-day-old seedlings were treated 0, 0.3 and 3.0 mM of MAA containing 5 mM in growth medium. Nitrate uptake was determined by following depletion from the uptake solution. The activity of the enzymes related to the process of reduction (NR: nitrate reductase; NiR: nitrite reductase; GS: glutamine synthetase) and the content of were analyzed in shoots and roots. The results of this study showed that uptake was inhibited 38% with treatment of 0.3 mM of MAA. However, there was more than three times increase of N03- uptake in 3.0 mM MAA. In addition, the enzymatic activities were positively affected by the high MAA rate. Finally, the content was increased slightly both in shoots and roots of leafy radish by MAA treatments.
 Keywords
leafy radish;nitrate uptake;amino acid;nitrate assimilation;
 Language
English
 Cited by
1.
아미노산 비료가 크리핑 벤트그래스의 생육과 질소 흡수에 미치는 영향,김영선;함선규;이재필;황영수;이규승;

Weed & Turfgrass Science, 2014. vol.3. 3, pp.246-252 crossref(new window)
1.
Effect of Amino Acid Fertilization on Nitrate Assimilation of Leafy Radish and Soil Chemical Properties in High Nitrate Soil, Communications in Soil Science and Plant Analysis, 2007, 39, 1-2, 269  crossref(new windwow)
2.
Evaluation of the Role of Mixed Amino Acids in Nitrate Uptake and Assimilation in Leafy Radish by Using 15N-Labeled Nitrate, Agricultural Sciences in China, 2008, 7, 10, 1196  crossref(new windwow)
3.
Effects of Two Amino Acid Fertilizers on Growth of Creeping Bentgrass and Nitrogen Uptake, Weed & Turfgrass Science, 2014, 3, 3, 246  crossref(new windwow)
 References
1.
Sivasankar, S., Rothstein, S., and Oaks A. (1997) Regulation of the accumulation and reduction of nitrate by nitrogen and carbon metabolites in maize seedlings. Plant Physiol. 114, 583-589

2.
Taiz, L. and Zeiger, E. (2002) Plant Physiology. 3rd Edition. Sinauer Associates, Inc., Sunderland, USA, P. 260-287

3.
Campbell, W.H. (1999) Nitrate reductase structure, function and regulation: bridging the gap between Biochemistry and Physiology. Annu. Rev. Plant Physiol. Mol. Biol. 50, 277-303 crossref(new window)

4.
Ford, B.G. (2000) Nitrate trnsporters in plants: structure, function and regulation. Biochimica et Biophysic Acta. 1465, 219-235 crossref(new window)

5.
Chapin, F.S., Moilainen, L., and Kielland, K. (1993) Preferential use of organic nitrogen by a non-mycorrhizal arctic sedge. Nature, 361, 150-153 crossref(new window)

6.
Matsumoto, S., Ae, N., and Yamagata, M. (2000) Possible direct uptake of organic nitrogen from soil by chingensai (Brassica campestris L.) and carrot (Daucus carota L.). Soil biology & Biochemistry. 32, 1301-1310 crossref(new window)

7.
Persson, J. and Nasholm, T. (2002) Regulation of amino acid uptake in conifers by exogenous and endogenous nitrogen. Planta. 215, 639-644 crossref(new window)

8.
Persson, J. and Nasholm T. (2001) Amino acid uptake: a Widespread ability among boreal forest plants. Ecol Lett. 4, 434-438 crossref(new window)

9.
Muller, B. and Touraine, B. (1992) Inhitition of $NO_3^-$ uptake by various phloem-translocated amino acids in soybean seedling. J. Exp. Bot. 43, 617-623 crossref(new window)

10.
Rodgers, C.O. and Barneix, A.J. (1993) The effect of amino acids and amides on the regulation of nitrate uptake by wheat seedlings. J. Plant Nutr. 16, 337-348 crossref(new window)

11.
Li, X.Z., Larson, D.E., Glibetic, M., and Oaks, A. (1995) Effect of glutamine on the induction of nitrate reductase. Physiol plant. 93, 740-744 crossref(new window)

12.
Padgett, P.E. and Leonard, R.T. (1996) Free amino acid levels and the regulation of nitrate uptake in maize cell suspension cultures. J. Exp. Bot. 47, 871-883 crossref(new window)

13.
Ivashikian, N.V. and Sokolov, O. A. (1997) Regulation of nitrate, nitrite, ammonium and glutamate. Plant Science. 123, 29-37 crossref(new window)

14.
Tillard, P., Passama, L., and Gojon, A. (1998) Are phloem amino acids involved in the shoot to root control of $NO_3^-$ uptake in Ricinus communis plants? J. Exp. Bot. 49, 1371-1379 crossref(new window)

15.
Aslam, M., Travis, R.L., and Rains, D.W. (2001) Differential effect of amino acids on nitrate uptake and reduction systems in barley roots. Plant Science. 160, 219-228 crossref(new window)

16.
Pal'ove-Balang, P. (2002) Role of nitrogen metabolites on the regulation of nitrate uptake in maize seedlings. Acta Biologica Szegediensis. 46, 177-178

17.
Cerezo, M., Flors, V., Legaz, F., and Garcia-Agustin, P. (2000) Characterization of the low affinity transport system for $NO_3^-$ uptake by Citrus roots. Plant Science. 160, 95-104 crossref(new window)

18.
Daniel-Vedele, F., Filleur, S., and Caboch, M. (1998) Nitrate transport: a key step in nitrate assimilation. Current Opinion in Plant Biology. 1, 235-239 crossref(new window)

19.
Deng, M.D., Moureaux, T., Cherel, I., Boutin, J.P., and Caboche, M. (1991) Effects of nitrogen metabolites on the regulation and circadian expression of tobacco nitrate reductase. Plant Physiol. Biochem. 29, 139-247

20.
Vicentz, M., Moureaux, T., Leydecker, M.T., Vaucheret, H., and Caboche, M. (1993) Regulation of nitrate and nitrite reductase expression in Nicotiana plumbaginifolia leaves by nitrogen and carbon metabolites. Plant J. 3, 315-324 crossref(new window)

21.
Kim, Y. S. (2002) The effect of mixed amino acid on nitrate uptake in rice, pea, cucumber and red pepper. Master of Agriculture Degree Thesis, Department of Agricultural Chemistry, College of Agriculture and Biotechnology, Chungnam National University, Daejon, Korea

22.
Ruiz, J. M., Rivero, R. M., Garcia, P. C., Baghour, M., and Romero R. (1999) Role of $CaCl_2$in nitrate assimilation in leaves and roots of tobacco plants (Nicotiana tabacum L.). Plant Science. 141, 107-115 crossref(new window)

23.
Cataldo, D.A., Haroon, M., Schrader, L.E., and Young, V.L. (1975) Rapid colorimetric determination of nitrate in plant tissue by nitration of salicylic acid. Comm. Soil Sci. Plant Anal. 6, 71-80 crossref(new window)

24.
Kaiser, J.J. and Lewis, O.A.H. (1984) Nitrate reductase and glutamine synthetase activity in leaves and roots of nitrate fed Helianthus annuus L. Plant Soil. 70, 127-130

25.
Gunes, A., Post, W.H.K., Kirkby, E.A., and Akas, M. (1994) Influence of partial replacement of nitrate by amino acid nitrogen or urea in the nutrient medium on nitrate accumulation in NFT grown winter lettuce. J. Plant Nutr. 17, 1929-1938 crossref(new window)

26.
Gunes, A., Inal, A., and Aktas, M. (1996) Reducing nitrate content of NFT grown winter onion plants (Allium cepa L.) by partial replacement of $NO_{3}$ with amino acid in nutrient solution. Scientia Horticulturae, 65, 203-208 crossref(new window)

27.
Chen, G. and Gao X. (2002) Effect of partial replacement of nitrate by amino acid and urea on nitrate content of nonheading Chinese cabbage and lettuce in hydroponics (Chinese). Scientia Agricultura Sinica, 35, 187-191

28.
Wang, H., Wu, L., and Tao, Q. (2004) Influence of partial replacement of nitrate by amino acids on nitrate accumulation of pakchoi (Brassica chinensis L.) (Chinese). China Environmental Science, 24, 19-23

29.
Radin, J.W. (1975) Differential regulation of nitrate reductase induction in roots and shoots of cotton plants. Plant Physiol. 55, 178-182 crossref(new window)

30.
Radin, J.W. (1977) Amino acid interactions in the regulation of nitrate reductase induction in cotton roots tips. Plant Physiol. 60, 467-469 crossref(new window)

31.
Oaks, A., Stulen, I., and Boesel, I. (1979) Influence of amino acids and ammonium on nitrate reduction in com seedlings. Can. J. Bot. 57, 1824-1829 crossref(new window)

32.
Oaks, A. (1994) Primary nitrogen assimilation in higher plants and its regulation. Can. J. Bot. 72, 739-750 crossref(new window)

33.
Bameix, A.J. and Causin, H.P. (1996) The central role of amino acids on nitrogen utilization and plant growth. J. Plant Physiol. 149, 358-362 crossref(new window)

34.
Pessarakli, M. (2002) Handbook of plant and crop physiology. 2nd Edition. Marcel Dekker, Inc., New York, USA, P. 390

35.
Barneix, A.J., James, D.M., Watson, E.F., and Hewitt, E.J. (1984) Some effects of nitrate abundance and starvation on metabolism and accumulation of nitrogen in barley (Hordeum vulgare L.) cv. Sonja. Planta. 162, 465-476