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Determination of Abamectin Residue in Paprika by High-Performance Liquid Chromatography
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 Title & Authors
Determination of Abamectin Residue in Paprika by High-Performance Liquid Chromatography
Xie, Wen-Ming; Ko, Kwang-Yong; Kim, Sung-Hun; Chang, Hee-Ra; Lee, Kyu-Seung;
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Reversed-phase high-performance liquid chromatography (HPLC) techniques were developed to quantify abamectin (ABM) in paprika (Capsicum annum). Separation was achieved on a ODS column with a mobile phase of acetonitrile/water (96/4, v/v) mixture in an isocratic elution at the flow tate of 1.2 mL/min for avermectins (AVMs). The retention times were 8.0 and 9.7mins for AVM and AVM , respectively. Residual AVMs (sum of AVM , AVM and 8,9-Z-AVM ) in the vegetable were extracted with acetonitrile, and the silica solid-phase extraction cartridges were used to purify the extract. AVMs were derivatized using trifluoroacetic acid and 1-methylimidazole, and the derivatives were determined with a fluorescence detector (excitation at 365 nm and emission at 470 nm). High and consistent recoveries, ranging from 93% to 115%, were obtained for AVM and 8, 9-Z-AVM at fortified levels of for paprika. The limit of quantitation (LOQ) was . The residual levels of AVMs in paprika in a field experiment from one day to seven days after the last application decreased from 18.40 to . The half-life of AVMs in paprika was 1.47 days.
Avermectin;paprika;residue;HPLC;fluorescence detection;
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