JOURNAL BROWSE
Search
Advanced SearchSearch Tips
Determination of Abamectin Residue in Paprika by High-Performance Liquid Chromatography
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
Determination of Abamectin Residue in Paprika by High-Performance Liquid Chromatography
Xie, Wen-Ming; Ko, Kwang-Yong; Kim, Sung-Hun; Chang, Hee-Ra; Lee, Kyu-Seung;
  PDF(new window)
 Abstract
Reversed-phase high-performance liquid chromatography (HPLC) techniques were developed to quantify abamectin (ABM) in paprika (Capsicum annum). Separation was achieved on a ODS column with a mobile phase of acetonitrile/water (96/4, v/v) mixture in an isocratic elution at the flow tate of 1.2 mL/min for avermectins (AVMs). The retention times were 8.0 and 9.7mins for AVM and AVM , respectively. Residual AVMs (sum of AVM , AVM and 8,9-Z-AVM ) in the vegetable were extracted with acetonitrile, and the silica solid-phase extraction cartridges were used to purify the extract. AVMs were derivatized using trifluoroacetic acid and 1-methylimidazole, and the derivatives were determined with a fluorescence detector (excitation at 365 nm and emission at 470 nm). High and consistent recoveries, ranging from 93% to 115%, were obtained for AVM and 8, 9-Z-AVM at fortified levels of for paprika. The limit of quantitation (LOQ) was . The residual levels of AVMs in paprika in a field experiment from one day to seven days after the last application decreased from 18.40 to . The half-life of AVMs in paprika was 1.47 days.
 Keywords
Avermectin;paprika;residue;HPLC;fluorescence detection;
 Language
English
 Cited by
1.
Effects of light shading and climatic conditions on the metabolic behavior of flonicamid in red bell pepper, Environmental Monitoring and Assessment, 2016, 188, 3  crossref(new windwow)
 References
1.
Burg, R. W., Miller, B. M., Baker, E. E., Birnbaum, J., Currie, S. A. and Hartman, R. (1979) A new family of potent anathematic agents: producing organism and fermentation. Antimicrobial Agents and Chemotherapy 5, 361-367

2.
Putter, I., MacConnell, J. G., Preiser, F. A. and Haidri, A. A. (1981) The efficacy of avemectin against citrus pests. Experientia 3, 7963-7968

3.
Roth, M., Rae, G. McGill, A. S. and Young, K. W. (1993) Ivermectin depuration in Atlantic salmon (Salmo salar). Journal of Agricultural and Food Chemistry 41(12), 2434-2436 crossref(new window)

4.
Wislocki, P. G., Grosso, L. S. and Dybas, R. A. (1989) Environmental aspects of abamectin use in crop protection. In: Campbell WC (ed) Ivermectin and abamectin. Springer Verlag, New York, p, 185-200

5.
Johan, V. (1991) Rapid determination of abamectin in lettuce and cucumber using high-performance liquid chromatography. Journal of Agricultural and Food Chemistry 39, 303-305 crossref(new window)

6.
Diserens, H. and Henzelin, M. (1999) Determination of abamectin residues in fruits and vegetables by high-performance liquid chromatography. Journal of Chromatography A 833, 13-18 crossref(new window)

7.
Roudaut, B. (1998) Multiresidue method for the determination of avermectin and moxidectin residues in the liver using HPLC with fluorescence detection. Analyst 123, 2541-2544

8.
Lucija, K., Jernej, K. and Nevenka, K. E. (2004) Determination of abamectin and doramectin in sheep faeces using HPLC with fluorescence detection. Biomedical Chromatography 18, 117-124 crossref(new window)

9.
Sari, P., Sun, J. G. and Razzak, M. (2006) HPLC assay of levamisole and abamectin in sheep plasma for application to pharmacokinetic studies. Journal of Liquid Chromatography & Related Technologies 29, 2277-2290 crossref(new window)

10.
Dusi, G., Faggionato, E., Bertocch,i L., Facchett,I. S. and Baiguera M. (2001) Determination of avermectin and milbemycin residues in milk for human consumption using liquid chromatography with fluorescence detection. Ind Alment-Italy 40, 11-14

11.
Sherri, B. T., Josze, E. R. and Wendy, C. A. and Laura, R. K. (2005) Analysis of avemectin and moxidectin residues in milk by liquid chromatography– tandem mass spectrometry using an atmospheric pressure chemical ionization/atmospheric pressure photoionization source. Analytica Chimica Acta 529, 159-165 crossref(new window)

12.
Sheridan, R. and Desjardins, L. (2006) Determination of abamectin, doramectin, emamectin, eprinomectin, ivermectin, and moxidectin in milk by liquid chromatography electrospray tandem mass spectrometry. Journal of AOAC International 89, 1088- 1094

13.
Wu, Z., Li, J., Zhu, L., Luo, H. and Xu, X. (2001) Multi-residue analysis of avermectins in swine liver by immunoaffinity extraction and liquid chromatography–mass spectrometry. Journal of Chromatography B 755, 361-366 crossref(new window)

14.
Hou, X. L., Li, X. W., Ding, S. Y., He, J. H., Jiang, H. Y. and Shen, J. Z. (2006) Simultaneous analysis of avemectins in bovine tissues by LC-MS-MS with immunoaffinity chromatography cleanup. Chromatographia 63, 543-550 crossref(new window)

15.
Hernandez, F., Pozo, O. J., Sancho, J. V., Bijlsma, L., Barreda, M. and Pitarch E. (2006) Multiresidue liquid chromatography tandem mass spectrometry determination of 52 non gas chromatographyamenable pesticides and metabolites in different food commodities. Journal of Chromatography A 1109, 242-252 crossref(new window)

16.
Shi, W. M., He, J. H. and Jiang, H. Y. (2006) Determination of multiresidue of avermectins in bovine liver by an indirect competitive ELISA. Journal of Agricultural and Food Chemistry 54, 6143- 6146 crossref(new window)

17.
Prabhu, S. V., Varsolona, R. J., Wehner, T.A., Egan, R. S and Tway, P. C. (1992) Rapid and sensitive high-performance liquid chromatographic method for the quantitation of abamectin and its delta 8,9-isomer. Journal of Agricultural and Food Chemistry 40, 622-625 crossref(new window)

18.
Vuik, J. (1991) Rapid determination of abmectin in lettuce and cucumber using high-performance liquid chromatography. Journal of Agricultural and Food Chemistry 39, 303-309 crossref(new window)

19.
Fong, W. G., Moye, H. A., Siber, J. N. and Toth, J. P. (1999)Pesticides residues in food, John Wiley & Sons, New York, USA.p,42-43