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Assessment of the Persistence of DNA in Decomposing Leaves of CMVP0-CP Transgenic Chili Pepper in the Field Conditions
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 Title & Authors
Assessment of the Persistence of DNA in Decomposing Leaves of CMVP0-CP Transgenic Chili Pepper in the Field Conditions
Lee, Bum-Kyu; Kim, Chang-Gi; Park, Ji-Young; Park, Kee-Woong; Yi, Hoon-Bok; Harn, Chee-Hark; Kim, Hwan-Mook;
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This study was conducted to evaluate the persistence of DNA in the transgenic chili pepper resistant to cucumber mosaic virus (CMV) in the field condition. We analyzed the persistence of genes in the leaf samples obtained from two field conditions, below and above soil. Transgenic and non-transgenic leaf tissues were buried in the soil at a depth of 10 cm or placed on the soil surface. Qualitative and quantitative PCR analysis showed that the amount of transferred genes from the transgenic peppers below and above soil was dropped to 28.3-42.7% one month after buried and it was rapidly reduced to 0.9-3.3% after two months. The transgenes were not detected three to four month after buried. In addition, DNA of the leaves placed below soil decomposed about 8%more than those on soil surface. The gene transfer from decomposing leaves of the transgenic pepper to soil was investigated by PCR analysis with the soil attached to the samples. The PCR result indicated that the gene transfer from the transgenic pepper to soil was not occurred.
DNA persistence;chili pepper;gene transfer;soil;transgenic plant;
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