Advanced SearchSearch Tips
Involvement of Oxidative Stress in Formaldehyde-induced Apoptosis in Cultured Lung Macrophage Cells
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
Involvement of Oxidative Stress in Formaldehyde-induced Apoptosis in Cultured Lung Macrophage Cells
Park, Soo-Hyun;
  PDF(new window)
Formaldehyde (FA) is an important irritant compound in pesticide to induce asthma and allergy in respiratory system. Alveolar macrophage is also an pivotal cell in the immune response of respiratory system. However, the effect of FA in macrophage cell viability has not been elucidated. Thus, this study was conducted to investigate the effect of FA on apoptosis in Raw 264.7 cells, alveolar macrophage cell line. In this study, FA decreased cell viability of lung alveolar macrophage cells in a dose-dependent manner (>). FA-induced decrease of cell viability was blocked by the treatment of antioxidants (vitamin C, NAC, and catalase). Indeed, FA induced lipid peroxide formation in Raw 264.7 cells. FA decreased Bcl-2 expression but increased Bax expression in lung alveloar macrophage cells. In addition, FA also increased the cleaved form of caspase-3. In conclusion, FA induced apoptosis via oxidative stress in cultured Raw 264.7 cells.
Alveolar macrophage;Apoptosis;Formaldehyde;Oxidative stress;
 Cited by
인간 간세포주 에서 포름알데히드에 의한 세포 사멸 효과,박수현;

한국환경농학회지, 2009. vol.28. 4, pp.462-467 crossref(new window)
Kampa, M. and Castanas, E. (2008) Human health effects of air pollution. Environ Pollut. 151(2), 362-367 crossref(new window)

Kita, T., Fujimura, M., Myou, S., Ishiura, Y., Abo, M., Katayama, N., Nishitsuji, M., Yoshimi, Y., Nomura, S., Oribe, Y., and Nakao, S. (2003) Potentiation Potentiation of allergic bronchoconstriction by repeated exposure to formaldehyde in guinea-pigs in vivo. Clin Exp Allergy 33, 1747-1753 crossref(new window)

Nakazawa, H., Ikeda, H., Yamashita, T., Hara, I., Kumai, Y., Endo, G. and Endo, Y. (2005) A case of sick building syndrome in a Japanese office worker. Ind Health. 43, 341-345 crossref(new window)

Bagchi, D., Bagchi, M., Hassoun, E.A., and Stohs, S. J. (1993) Detection of paraquat-induced in vivo lipid peroxidation by gas chromatography/mass spectrometry and high-pressure liquid chromatography. J. Anal. Toxicol. 17(7), 411-414 crossref(new window)

Callas, P.W., Pastides, H., and Hosmer, D. W. Jr. (1996) Lung cancer mortality among workers in formaldehyde industries. J. Occup. Environ. Med. 38(8), 747-748 crossref(new window)

Collins, J.J., Acquavella, J.F., and Esmen, N.A. (1997) An updated meta-analysis of formaldehyde exposure and upper respiratory tract cancers. J. Occup. Environ. Med. 39(7), 639-651 crossref(new window)

Marsh, G.M., Stone, R.A., Esmen, N.A., Henderson, V.L., and Lee, K.Y. (1996) Mortality among chemical workers in a factory where formaldehyde was used. Occup. Environ. Med. 53(9), 613-627 crossref(new window)

Kamdar, O., Le, W., Zhang, J., Ghio, A.J., Rosen, G. D, and Upadhyay, D. (2008) Air pollution induces enhanced mitochondrial oxidative stress in cystic fibrosis airway epithelium. FEBS Lett. 582, 3601-3606 crossref(new window)

Schneider, J. C., Card, G. L., Pfau, J. C., and Holian, A. (2005) Air pollution particulate SRM 1648 causes oxidative stress in RAW 264.7 macrophages leading to production of prostaglandin E2, a potential Th2 mediator. Inhal. Toxicol. 17, 871-877 crossref(new window)

Higychi, M., Hisgahi, N., Taki, H., and Osawa, T. (1990) Cytolytic mechanisms of activ ated macrophages. Tumor necrosis facto and L-arginine-dependent mechani는 act synergistically as the major cytolytic mechani는 of activated macrophages. J. Immunology. 144, 1425-1431

Khadaroo, R. G., Kapus, A., Powers, K.A., Cybulsky, M. I., Marshall, J. C., and Rotstein, O.D. (2003) Oxidative stress reprograms lipopolysaccharide signaling via Src kinase-dependent pathway in RAW 264.7 macrophage cell line. J. Biol. Chem. 278, 47834-47841 crossref(new window)

Imrich, A., Ning, Y., Lawrence, J., Coull, B., Gitin, E., Knutson, M., and Kobzik L. (2007) Alveolar macrophage cytokine response to air pollution particles: oxidant mechanisms. Toxicol. Appl. Pharmacol. 218, 256-264 crossref(new window)

Zinkel, S., Gross, A. and Yang, E. (2006) BCL2 family in DNA damage and cell cycle control. Cell Death Differ. 13(8), 1351-1359 crossref(new window)

Kutuk, O. and Basaga, H. (2007) Apoptosis signalling by 4-hydroxynonenal: a role for JNK-c-Jun/AP-1 pathway. Redox Rep. 12(1), 30-34 crossref(new window)

Zakeri, Z. and Lockshin, R. A. (2008) Cell death: history and future. Adv Exp Med Biol. 615, 1-11 crossref(new window)

Fujimaki, H., Kurokawa, Y., Kunugita, N., Kikuchi, M., Sato, F. and Arashidani, K. (2004) Differential immunogenic and neurogenic inflammatory responses in an allergic mouse model exposed to low levels of formaldehyde. Toxicology. 197, 1-13 crossref(new window)

Nakao, H., Umebayashi, C., Nakata, M., Nishizaki, Y., Noda, K., Okano, Y., and Oyama, Y. (2003) Formaldehyde-induced shrinkage of rat thymocytes. J. Pharmacol. Sci. 91(1), 83-86 crossref(new window)

Grafstrom, R. C., Fornace, A. J. Jr, Autrup, H., Lechner, J. F., and Harris, C. C. (1983) Formaldehyde damage to DNA and inhibition of DNA repair in human bronchial cells. Science. 220, 216-218 crossref(new window)

Shang, Y., Li, X., Prasad, P. V., Xu, S., Yao, S., Liu, D., Yuan, S., and Feng, D. (2009) Erythropoietin attenuates lung injury in lipopolysaccharide treated rats. J. Surg. Res. 155, 104-110 crossref(new window)

Wu, Y., Xing, D., Chen, W.R. and Wang, X. (2007) Bid is not required for Bax translocation during UV-induced apoptosis. Cell Signal. 19(12), 2468-2478 crossref(new window)

Cao, X., Bennett, R. L. and May, W. S. (2008) c-Myc and caspase-2 are involved in activating Bax during cytotoxic drug-induced apoptosis. J. Biol. Chem. 283(21), 14490-14496 crossref(new window)

Tong, Q. S., Zheng, L. D., Tang, S. T., Jiang, G. S., Ruan, Q. L., Zeng, F. Q., and Dong, J. H. (2007) Nitrofen suppresses cell proliferation and promotes mitochondria-mediated apoptosis in type II pneumocytes. Acta Pharmacol Sin. 28(5), 672-684 crossref(new window)

An, S., Hishikawa, Y., Liu, J., and Koji, T. (2007) Lung injury after ischemia-reperfusion of small intestine in rats involves apoptosis of type II alveolar epithelial cells mediated by TNF-alpha and activation of Bid pathway. Apoptosis. 12(11), 1989-2001 crossref(new window)

Tsukahara, S., Yamamoto, S., Tin-Tin-Win-Shwe, Ahmed, S., Kunugita, N., Arashidani, K., and Fujimaki, H. (2006) Inhalation of low-level formaldehyde increases the Bcl-2/Bax expression ratio in the hippocampus of immunologically sensitized mice. Neuroimmunomodulation. 13(2), 63-68 crossref(new window)

Susnow, N., Zeng, L., Margineantu, D., and Hockenbery DM. (2009) Bcl-2 family proteins as regulators of oxidative stress. Semin. Cancer Biol. 19(1), 42-49 crossref(new window)