Publisher : The Korean Society of Environmental Agriculture
DOI : 10.5338/KJEA.2010.29.1.072
Title & Authors
Development of Electro-Biosensor for the Residual Pesticides using Organic Carbon and Cobalt Phthalocyanine Yu, Young-Hun; Cho, Hyung-Jun; Park, Won-Pyo; Hyun, Hae-Nam;
We have developed the bio-electrode measuring the variance of the amount of acetylcholine affected by residual pesticide. The working electrode of the biosensor was made by combination of cobalt phthalocyanine and carbon organic compounds. The biosensors were constructed by screen-printing method. The principle of working electrode is similar to thiocholine sensor. We have fabricated the biosensor using standard screen printing method. Generally, the biosensor made by printing method formed thick film biosensor. When the electrodes were made by electrochemical cells, the generation of current by the addition of enzyme substrate was inhibited by standard solutions of organo-phosphate pesticides. The detection limit of sensor is about 0.5 for carbofuran. We could improve the responsibility of the sensor by controlling the cobalt phthalocyanine and thiocholine concentration ratio. Also we have tested the EPN and Chlorpyrifos pesticides and found that the biosensor is applicable to fast determination of residual pesticides.
Bruno, J.P., Sarter, M., Gash, C., and Parikh, V. (2006) Choline and acetylecholine-sensitive microelectrode, Encyclopedia of Sensor. 10, 1-15.
Cho, H., Yu, Y., and Hyun, H. (2005) Development of Biosensor reader system for the Residual Pest-icides using White light Sourece and Color-sensor, Hankook Kwanghak Hoeji. 8(16), 433-438.
Ciucu, A. (2001) Organic phase amperometric biosensor for detection of pesticides, Roum. Biotechnol. Lett. 7(2), 667-676.
Cui, G., Yoo, J., Lee, S., Nam, H., and Cha, G. (2000) Differential thick-film amperometric glucose sensor with an enzyme-immobilized nitrocellucose mem-brane, Electroanalysis. 13(3), 224-228.
Mitchell, K.M. (2004) Acetylecholine and choline amperometric enzyme sensor characterized in vitro and in vivo, Anal. Chemistry. 76(4), 1098-1106.
Parikh, V., Pomerleau, F., Huetti, P., Gerhardt, G.A., Sarter M., and Burno, J.P. (2004) Rapid assessment of in vivo cholinergic transmission by amperometric detection of change in extracellular choline levels, European J. Neuroscience. 20, 1545-1554.
Reshetilov, A.N. (2005) Biosensors 2004: The eighth world congress on biosensors, Appl. Biochem. Mi-crobiology. 41(2), 219-223.
Rich, Rebecca L., and Myszka, David G. (2005), Survey of the year 2003 commercial optical biosensor literature, J. Mol. Recognit. 18, 1-39.
Savran, C.A., Burg, T.P., Fritz, J., and Manalis, S.R. (2003) Microfabricated mechanical biosensor with inherently differential readout, Appl. Phys. Lett. 83(8), 1659-1661.
Spence, M.M., Rubin, S.M., Dimitrov, I.E., Janette Ruiz, E., Wemmer, D.E., Pines, A., Yaoi, S.Q., Tiani, F., and Schultzi, P.G. (2003) Functionalized xenon as a biosensor, PNAS. 98(19), 10654-10657.