JOURNAL BROWSE
Search
Advanced SearchSearch Tips
Physiological and Proteomics Analysis to Potassium Starvation in Rice
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
Physiological and Proteomics Analysis to Potassium Starvation in Rice
Kim, Sang-Gon; Wang, Yiming; Lee, Chang-Hoon; Chi, Yong-Hun; Kim, Keun-Ki; Choi, In-Soo; Kim, Yong-Chul; Kang, Kyu-Young; Kim, Sun-Tae;
  PDF(new window)
 Abstract
BACKGROUND: Potassium (K) is one of the macronutrients which are essential for plant growth and development. Its deficiency in paddy soils is becoming one of the limiting factors for increasing rice yield in Asia. METHODS AND RESULTS: To investigate physiological symptoms under K-starvation (NP) compared with complete media (NPK) condition, we measured shoot/root length, weight, nutrients, and patterns of protein expression. The shoot growth was significantly reduced, but root growth was not affected by K-starvation. However, biomasses were decreased in both shoot and root. Uptake of K was reduced up to 85%, while total concentrations of P, Ca, Mg, Na were increased in root and shoot. To better understand the starved K mechanism of rice, comparative proteome analysis for proteins isolated from rice leaves was conducted using 2-DGE. Five spots of differentially expressed proteins were analyzed by MALDI-TOF MS. Analysis of these K-starvation response proteins suggested that they were involved in metabolism and defense. CONCLUSION(s): Physiological and 2-DGE based proteomics approach used in our study results in observation of morphology or nutrients change and identification of K-starvation responsive proteins in rice root. These results have important roles in maintaining nutrient homeostasis and would also be useful for further characterization of protein function in plant K nutrition.
 Keywords
Potassium starvation;Proteomics;Rice;2-DGE;
 Language
English
 Cited by
 References
1.
Adams, F., 1971. Soil solution. In: The plant root and its environment. Edited by E.W. Carson, University Press of Virginia, Charlottesville, VA. 441-481.

2.
Amtmann, A., Armengaud, P., Volkov, V., 2004. Potassium nutrition and salt stress. In membrane transport in plants. Edited by M.R. Blatt, Blackwell Publishing, Oxford, 293‐339.

3.
Armengaud, P., Breitling, R., Amtmann, A., 2004. The potassium dependent transcriptome of Arabidopsis reveals a prominent role of jasmonic acid in nutrient signalling, Plant Physiol. 136, 2556 -2576. crossref(new window)

4.
Ashley, M.K., Grant, M., Grabov, A., 2006. Plant responses to potassium deficiencies: a role for potassium transport proteins, J. Exp. Bot. 57, 425-436.

5.
Bednarz, C.W., Oosterhuis, D.M., Evans, R.D., 1998. Leaf photosynthesis and carbon isotope discrimination of cotton in response to potassium deficiency, Environ. Exp. Bot. 39, 131-139. crossref(new window)

6.
Chen, R.D., Yu, L.X., Greer, A.F., Cheriti, H., Tabaeizadeh, Z., 1994. Isolation of an osmotic stress‐and abscisic acid induced gene encoding an acidic endochitinase from Lycopersiconchilense, Mol. Genet. Genomics 245, 195-202.

7.
Cho, K., Shibato, J., Agrawal, G.K., Jung, Y.H., Kubo, A., Jwa, N.S., Tamogami, S., Satoh, K., Kikuchi, S., Higashi, T., Kimura, S., Saji, H., Tanaka, Y., Iwahashi, H., Masuo, Y., Rakwal, R., 2007. Survey of differentially expressed proteins and genes in jasmonic acid treated rice seedling shoot and root at the proteomics and transcriptomics levels, J. Proteome Res. 7, 2980-2998.

8.
Dixon, R.A., Steele, C.L., 1999. Flavonoids and isoflavonoids-a gold mine for metabolic Engineering, Trends Plant Sci. 4, 394-400. crossref(new window)

9.
Dhugga, K.S., Tiwari, S.C., Ray, P.M., 1997. A reversibly glycosylated polypeptide (RGP1) possibly involved in plant cell wall synthesis: Purification, gene cloning, and trans‐Golgi localization, Proc. Natl. Acad. Sci. USA 94, 7679-7684. crossref(new window)

10.
Hong, J.K., Hwang, B.K., 2006. Promoter activation of pepper class II basic chitinase gene, CAChi2, and enhanced bacterial disease resistance and osmotic stress tolerance in the CAChi2‐overexpressing Arabidopsis, Planta 223, 433-448. crossref(new window)

11.
Laegreid, M., Bockman, O.C., Kaarstad, O., 1999. Agriculture, fertilizers and the environment. CABI, Oxon, UK.

12.
Leigh, R.A., Wyn Jones, R.G., 1984. A hypothesis relating critical potassium concentrations for growth to the distribution and function of this ion in the plant cell, New Phytologist 97, 1-13. crossref(new window)

13.
Marschner, H., 1995. Mineral Nutrition of Higher Plants, Ed 2. Academic Press, London.

14.
Mizuno, N., Minami, M., 1980. The use of $H_{2}SO_{4}-H_{2}O_{2}$ for the destruction of plants matter as a preliminary to determination of N, K, Mg, Ca, Fe, Mn, Jpn. J. Soil Sci. Plant Nutr. 51, 418-420.

15.
Karkonen, A., Murigneux, A., Martinant, J.P., Pepey, E., Tatout, C., Dudley, B.J., Fry, S.C., 2005. UDPglucose dehydrogenase of maize: A role in cell wall pentose biosynthesis, Biochem. J. 391, 409-415. crossref(new window)

16.
Kim, S.G., Kim, S.T., Kang, S.Y., Wang, Y., Kim, W., Kang, K.Y., 2008a. Proteomic analysis of reactive oxygen species (ROS)‐related proteins in rice roots, Plant Cell Rep. 27, 363-375. crossref(new window)

17.
Kim, S.G., Wang ,Y., Wu, J., Kang, K.Y., Kim, S.T., 2011. Physiological and proteomic analysis of young rice leaves grown under nitrogenstarvation conditions, Plant Biotechnol. Rep. 5, 309-315. crossref(new window)

18.
Kim, S.T., Kim, S.G., Hwang, D.H., Kang, S.Y., Kim, H.J., Lee, B.H., Lee, J.J., Kang, K.Y., 2004. Proteomic analysis of pathogen‐responsive proteins from rice leaves induced by rice blast fungus, Magnaporthe grisea , Proteomics 4, 3569-3578. crossref(new window)

19.
Kim, S.T., Kim, S.G., Kang, Y.H., Wang, Y., Kim, J.Y., Yi, N., Kim, J.K., Rakwal, R., Koh, H.J., Kang, K.Y., 2008b. Proteomics analysis of rice lesion mimic mutant (spl1) reveals tightly localized probenazole induced protein (PBZ1) in cells undergoing programmed cell death, J. Proteome Res. 7, 1750-1760. crossref(new window)

20.
Shin, R., Schachtman, D.P., 2004. Hydrogen peroxide mediates plant root cell response to nutrient deprivation, Proc. Natl. Acad. Sci. USA 101, 8827-8832. crossref(new window)

21.
Tabuchi, M., Sugiyama, K., Ishiyama, K., Inoue, E., Sato, T., Takahashi, H., Yamaya, T., 2005. Severe reduction in growth rate and grain filling of rice mutants lacking OsGS1;1, a cytosolic glutamine synthetase1;1, Plant J. 42, 641‐651.

22.
Véry, A.A., Sentenac, H., 2003. Molecular mechanisms and regulation of $K^{+}$ transport in higher plants, Annu. Rev. Plant Biol. 54, 575-603. crossref(new window)

23.
Wasaki, J., Yonetani, R., Kuroda, S., Shinano, T., Yazaki, J., Fujii, F., Shimbo, K., Yamamoto, K., Sakata, K., Sasaki, T., Kishimoto, N., Kikuchi, S., Yamagishi, M., Osaki, M., 2003. Transcriptomic analysis of metabolic changes by phosphorus stress in rice plant roots, Plant Cell Environ. 26, 1515-1523. crossref(new window)

24.
Watanabe, T., Osaki, M., Yoshihara, T., Tadano, T., 1998. Distribution and chemical speciation of aluminum in the Al accumulator plant Melastoma malabathricum L, Plant Soil 201, 165-173. crossref(new window)

25.
Winkel‐Shirley, B., 2001. Flavonoid biosynthesis. A colorful model for genetics, biochemistry, cell biology and biotechnology, Plant Physiol. 126, 485-493. crossref(new window)

26.
Yan, S., Tang, Z., Su, W., Sun, W., 2005. Proteomic analysis of salt stress‐responsive proteins in rice root, Proteomics 5, 235-244. crossref(new window)

27.
Zhao, D., Oosterhuis, D.M., Bednarz, C.W., 2001. Influence of potassium deficiency on photosynthesis, chlorophyll content, and chloroplast ultrastructure of cotton plants, Photosynthetica 39, 103-109. crossref(new window)