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Effects of Transgenic Soybean Cultivation on Soil Microbial Community in the Rhizosphere
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 Title & Authors
Effects of Transgenic Soybean Cultivation on Soil Microbial Community in the Rhizosphere
Lee, Ki-Jong; Sohn, Soo-In; Lee, Jang-Yong; Yi, Bu-Young; Oh, Sung-Dug; Kweon, Soon-Jong; Suh, Seok-Choel; Ryu, Tae-Hun; Kim, Kyung-Hwan; Park, Jong-Sug;
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 Abstract
BACKGROUND: Soybean [Glycine max (L.) Merrill] is a legume and an important oil crop worldwide. This study was conducted to evaluate the possible impact of transgenic soybean cultivation on the soil microbial community. METHODS AND RESULTS: Microorganisms were isolated from the rhizosphere soils. Microbial community was identified based on the culture-dependent and molecular biology methods. The total numbers of bacteria, fungi, and actinomycete in the rhizosphere soils cultivated with transgenic and non-transgenic soybeans were similar to each other, and there was no significant difference between transgenic and non-transgenic soybeans. Dominant bacterial phyla in the rhizosphere soils cultivated with transgenic or non-transgenic soybeans were Actinobacteria, Firmicutes, and Proteobacteria. The microbial communities in transgenic and non-transgenic soybean soils were characterized using the denaturing gradient gel electrophoresis (DGGE). The DGGE profiles showed the different patterns, but didn't show significant difference to each other at 0.05 significance level. DNAs were isolated from soils cultivating transgenic or non-transgenic soybeans and analyzed for persistence of transgenes in the soil by using PCR. PCR analysis revealed that there were no amplified -tmt and bar gene in soil DNA. CONCLUSION(S): The results of this study suggested that microbial community of soybean field were not significantly affected by cultivation of the transgenic soybeans.
 Keywords
Gene transfer;Soil microbial community;Transgenic soybean;
 Language
Korean
 Cited by
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The GMO Industry: A Neglected Earthly Frontier, Journal of Hunger & Environmental Nutrition, 2016, 1  crossref(new windwow)
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