Advanced SearchSearch Tips
Selection of Antagonistic Microorganisms against Plant Pathogens from Eco-friendly Formulations
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
Selection of Antagonistic Microorganisms against Plant Pathogens from Eco-friendly Formulations
Gang, Guen-Hye; Cha, Jae-Yul; Heo, Bit-Na; Yi, Og-Sun; Lee, Yong-Bok; Kwak, Youn-Sig;
  PDF(new window)
BACKGROUND: Some microorganisms extant in nature have ability to suppress various plant pathogens, and also can promote plant growth. Thus microorganisms are such great source of antimicrobial agents to develop antagonistic microorganism production and eco-friendly crop management. We isolated the microorganisms in various eco-friendly formulations. The suppressive abilities against plant pathogens have been characterized in vitro level. METHODS AND RESULTS: The indigenous microorganisms have been isolated from Cooked rice, Black sugar, Rice Bran, and Red clay using dilution plating method. Population of bacteria and fungi were above 107 in the all formulations. We isolated and pure cultured the microorganisms based on morphological characteristics. Three major plant pathogens (Fusarium oxysporum, Rhizoctonia solani, Phytophthora capsici) have been used to select antagonistic microorganisms. Total 20 bacteria and 9 fungi showed the pathogen growth suppression ability in vitro condition. The selected microorganisms were identified by ITS sequence similarity. CONCLUSION: All tested eco-friendly formulations contained high-density of the microorganisms. Among the isolated microorganisms, Bacillus spp. and Streptomyces spp. showed the most effective antifungal activity against the plant pathogens such as F. oxysporum, R. solani, and P. capsici. Among the selected fungi Trichoderma sp. demonstrated antifungal activity. Our results suggest that the currently adapted eco-friendly formulations might useful for sustain agricultural system.
Antagonistic microorganism;Black sugar;Cooked rice;Eco-friendly formulations;Red clay;Rice bran;
 Cited by
미강제제 처리가 밭재배 고추의 생육, 이화학적 특성, 미생물 군집 및 시들음병 방제에 미치는 영향,이정한;강근혜;박경미;이용복;곽연식;

농업생명과학연구, 2014. vol.48. 1, pp.41-48 crossref(new window)
친환경제제 처리에 의한 토양미생물 군집 변화 및 벼잎집무늬마름병 (Rhizoctonia solani) 억제효과,강근혜;이정한;박경미;이용복;곽연식;

농업생명과학연구, 2014. vol.48. 4, pp.47-53 crossref(new window)
반유한 직립형 동부의 하우스 파종기 이동에 따른 농업적 형질의 변화,김동관;손동모;이경동;임요섭;정정성;

한국작물학회지, 2014. vol.59. 4, pp.470-476 crossref(new window)
친환경농자재 개발을 위한 키토산-알긴산비드 제조 및 응용,강하린;정우진;

한국키틴키토산학회지, 2016. vol.21. 3, pp.158-165 crossref(new window)
Alexander, M., 1982. Most probable number method for microbial populations, in; A. L. Page, R. H. Miller, D. R. Keeney (eds.), In methods of soil Analysis, Part 2: Chemical and microbiological properties. American Society of Agronomy, Inc., Soil Science Society of America, Inc., Madison, Wisconsin, pp. 815-820.

Baik, S. Y., Kim, J. C., Jang, K. S., Choi, Y. H. Choi,G. J., 2011. Development of efficient screening method for resistance of cabbage to Fusarium oxysporum f. sp.Conglutinans, Research. Plant Diseases 17, 13-18. crossref(new window)

Cho, D. H., Kang, J. Y. Yu, Y. H., 2004. Anastomosis group, pathogenicitiy and growth characteristics of Rhizoctonia solanicausing damping-off on Panax ginseng, Journal Ginseng 28, 183-190. crossref(new window)

Jeong H. Y., Lim, J. H., Kim, B. K., Lee, J. J., Kim, S. D., 2010. Selection and mechanisms of indigenous antagonistic microorganisms against sheath rot and dry rot disease of Garlic, Korean Journal of Microbiology and Biotechnology 38, 295-301.

Jung, H. K., Kim, J. R., Kim, B. K., Yu, T. S., Kim, S. D., 2005. Selection and antagonistic mechanism of Bacillus thuringiensis BK4 against Fusarium wilt disease of tomato, Korean Journal of Microbiology and Biotechnology 33, 194-199.

Karthikeyan, V., Patharajan, S., Palani, P., Spadaro, D., Gullino, M. L., Garibaldi, A., 2010. Modified simple protocol for efficient fungal DNA extraction highly suitable for PCR aased molecular nethods, Global Journal of Molecular Sciences 5, 37-42.

Kim, K. K., Kim, Y. C., Choi, Y. W., Park, K. D., Kang, U. G., Choi, Y. L. Park, H. C., 2008. Biological control of plant pathogens by Bacillus sp. AB02., Journal of Life Science 18, 858-864. crossref(new window)

Kim, D. H., Kim, S. H., 1999. Biochemical characteristics of whole soybean cereals fermented with Mucor and Rhizopus strains, Korean Journal of Food Science and Technology 31, 176-182.

Kim, S. G., Jang, Y., Kim, H. Y., Koh, Y, J., Kim, Y. H., 2010. Comparison of microbial fungicides in antagonistic activities related to the biological control of Phytophthora blight in Chili pepper caused by Phytophthora capsici, Plant Pathology J. 26,340-345. crossref(new window)

Lee, G. W., Kim, M. J., Park, J. S., Chae, J. C., Soh, B. Y., Ju, J. E., Lee, K. J., 2011. Biological control of phytophthora blight and anthracnose disease in red-pepper using bacillus subtilis S54, Research in Plant Disease 17, 86-89. crossref(new window)

Lee, K. Y., Kang, H. J., Youn, K. H., Ahn, K. S., Min, K. B., Bu, G. P., 1998. Rhizoctonia black stem rot of saururi herba caused by Rhizoctonia solani, Korean Journal of Plant Pathology 14, 184-185.

Lee, Y. S., Chang, T. H., Ryu, Y. J., Park, J. Y., Lim, T. H., 2005. Efficacy of suppression of Phytophthora Blight of red pepper caused by Phytophthora capsici by treatment with antagonistic Trichoderma harzianum DYMC in greenhouse, Korean Journal of Environmental Agriculture 24, 409-415. crossref(new window)

Lim, T. H., Cho, S. H., Kim, J. H., 2007. Effects of Streptomyces sp. MG 121 on growth of pepper plants and antifungal activity, Research in Plant Disease 13, 93-97. crossref(new window)

Lim, S. L., Yoo, J. Y., 1999. Characteristics of fungal protease produced by Mucor racemosus f. racemosus from korean traditional meju, Korean Journal of Applied Microbiology and Biotechnology 27, 466-470.

Oh, J. Y., Lee, Y. H., Jin, Y. D., Kim, J. B., Hwang, S. G., Han, S. H., Kim, J. E., 2007. Antifungal activity of pestcides to control dry rot and blue mold during garlic storage, The Korean Journal of Pestcide Science 11, 331-338.

White T.J., Bruns T., Lee, S., and Taylor, J. W., 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics, in: Innis, M.A., Gelfand, D.H., Sninsky, J.J., White, T.J., (Eds), PCR protocols: a guide to methods and applications, New York Academic Press, USA, pp. 315-33.