Publisher : The Korean Society of Environmental Agriculture
DOI : 10.5338/KJEA.2013.32.1.48
Title & Authors
Development of Proteomics-based Biomarkers for 4 Korean Cultivars of Sorghum Seeds (Sorghum bicolor (L.) Moench) Kim, Jin Yeong; Lee, Su Ji; Ha, Tae Joung; Park, Ki Do; Lee, Byung Won; Kim, Sang Gon; Kim, Yong Chul; Choi, In Soo; Kim, Sun Tae;
BACKGROUND: Sorghum (Sorghum bicolor (L.) Moench) ranks as the 6th most planted crop in the world behind wheat, rice, maize, soybean, and barley. The objective of this study was to identify bio-marker among sorghum cultivars using proteomics approach such as two-dimensional polyacrylamide gel electrophoresis (2-DE) coupled with mass spectrometry (MS). METHODS AND RESULTS: Proteins were extracted from sorghum seed, and separated by 2-DE. Total 652 spots were detected from 4 different sorghum seed after staining of 2-DE with colloidal Coomassie brilliant blue (CBB). Among them, 8 spots were differentially expressed and were identified using MALDI-TOF/TOF mass spectrometry. They were involved in RNA metabolism (spot1, spot 4), heat shock proteins (HSPs, spot 2), storage proteins (spot 3, spot 5, and spot 6), and redox related proteins (spot 8). Eight of these proteins were highly up-regulated in Whinchalsusu (WCS). The HSPs, Cupin family protein, and Globulin were specifically accumulated in WCS. The DEAD-box helicase was expressed in 3 cultivars except for WCS. Ribonuclease T2 and aldo-keto reductase were only expressed in 3 cultivars except for Daepung-susu (DPS). CONCLUSION(S): Functions of identified proteins were mainly involved in RNA metabolism, heat shock protein (HSP), and redox related protein. Thus, they may provide new insight into a better understanding of the charactreization between the cultivars of sorghum.
Awika, J.M., Rooney, L.W., Wu, X., Prior, R.L., Cisneros‐ Zevallos, L., 2003. Screening methods to measure antioxidant activity of sorghum (Sorghum bicolor) and sorghum products, J. Agric. Food Chem. 51, 6657‐6662.
Breiteneder, H., Mills, E.N., 2005. Plant food allergens-structural and functional aspects of allergenicity, Biotechnol. Adv. 23, 395-399.
Chang, H.G., Park, Y.S., 2005. Effects of waxy and normal sorghum flours on sponge cake properties, Food Engine. Prog. 9, 199‐207.
Cho, N.K., Kang, Y.K., Song, C.K., Jeun, Y.C., Oh, J.S., Cho, Y.I., S.J., Park, 2004. Effects of planting density on growth, forage yield and chemical composition of Jeju native Sorghum (Sorghum bicolor L.), J. Korean Grassl. Sci. 24, 225‐230.
De la Cruz, J., Kressler, D., Linder, P., 1999. Unwinding RNA in Saccharomyces cerevisiae: DEAD‐box proteins and related families, Trends Biochem. Sci. 24, 192-198.
Dunwell, J.M., Culham, A., Carter, C.E., Sosa‐Aguirre, C.R., Goodenough, P.W., 2001. Evolution of functional diversity in the cupin superfamily, Trends Biochem. Sci. 26, 740-746.
Jogeswar, G., Ranadheer, D., Anjaiah, V., Kishor, P.B.K., 2007. High frequency somatic embryogenesis and regeneration in different genotypes of Sorghum bicolor (L.) Moench from immature inflorescence explants, In Vitro Cell Dev. Biol. Plant 43, 159‐166.
Kil, H.Y., Seong, E.S., Ghimire, B.K., Chung, I.M., Kwon, S.S., Goh, E.J., Heo, K., Kim, M.J., Lim, J.D., Lee, D., Yu, C.Y., 2009. Antioxidant and antimicrobial activities of crude sorghum extract, Food Chem. 115, 1234‐1239.
Kim, K.O., Kim, H.S., Ryu, H.S., 2006. Effect of Sorghum bicolor L. Moench (sorghum, su‐su) water extracts on mouse immune cell activation, J. Korean Diet Assoc. 12, 82‐88.
Kim, S.T., Cho, K.S., Jang, Y.S., Kang, K.Y., 2001. Two-dimensional electrophoresis analysis of rice proteins by polyethylene glycol fractionation for protein arrays, Electrophoresis 22, 2103‐2109.
Kim, S.T., Kim, S.G., Hwang, D.H., Kang, S.Y., Kim, H.J., Lee, B.H., Lee, J.J., Kang, K.Y., 2004. Proteomic analysis of pathogen‐responsive proteins from rice leaves induced by rice blast fungus, Magnaporthe grisea, Proteomics 4, 3569-3578.
Kim, U.G., Jung, H.J., Lee, S.J., Kim, S.T., 2012. Crop proteomics: Practical method for high resolution of two‐dimensional electrophoresis, J. Plant Biotechnol. 39, 81‐92.
Kumar, S.A., Alam, S.I., Sengupta, N., Sarin, R., 2011. Differential proteomic analysis of salt stress response in Sorghum bicolor leaves. Environ. Exp. Bot. 71, 321-328.
Lee, K.E., Lee, J.Y., Kim, K., 2008. Effect of content of crop component on the bioethanol production, Korean J. Crop Sci. 53, 339‐346.
Lorsch, J.R., 2002. RNA chaperones exist and DEAD box proteins get a life, Cell 109, 797-800.
Maqbool, A., Zahur, M., Irfan, M., Qaiser, U., Rashid, B., Husnain, T., Riazuddin, S., 2007. Identification, characterization and expression of drought related alpha‐crystalline heat shock protein gene (GHSP) from desi cotton (Gossypium arboreum L.), Crop Sci. 47, 2437‐2444.
Ndimba, B.K., Thomas, L.A., Ngara, R., 2010. Sorghum 2‐dimensional proteome profiles and analysis of Hsp70 expression under salinity stress. Kasetsart J. (Nat Sci) 44: 768-775.
Pandey, A., Mann, M., 2000. Proteomics to study genes and genomes. Nature 405, 837‐846.
Rabilloud, T., 2002. Two‐dimensional gel electrophoresis on proteomics: Old, old fashioned, but it still climbs up the mountain, Proteomics 2, 3-10.
Spite, M., Baba, S.P., Ahmed, Y., Barski, O.A., Nijhawan, K., Petrash, J.M., Bhatnagar, A., Srivastava, S., 2007. Substrate specificity and catalytic efficiency of aldoketo reductases with phospholipid aldehydes, Biochem. J. 405, 95-105.
Tanner, N.K., Linder, P., 2001. DExD/H box RNA helicases: Fromgeneric motors to specific dissociation functions, Mol. Cell 8, 251-262.
Taylor, C.B., Bariola, P.A., Delcardayre, S.B., Raines, R.T., Green, P.J., 1993. RNS2: a senescence‐associated RNAse of Arabidopsis that diverged from the sRNAses before speciation. Proc. Natl. Acad. Sci. USA90, 5118‐5122.
Vashisht, A.A., Tuteja, N., 2006. Stress responsive DEAD-box helicases: a new pathway to engineer plant stress tolerance, J. Photochem. Photobiol. B 84,150-160.