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Impact of Virus-resistant Trigonal Cactus Cultivation on Soil Microbial Community
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 Title & Authors
Impact of Virus-resistant Trigonal Cactus Cultivation on Soil Microbial Community
Oh, Sung-Dug; Kim, Jong-Bum; Lee, Jung-Jin; Kim, Min-Kyeong; Ahn, Byung-Ohg; Sohn, Soo-In; Park, Jong-Sug; Ryu, Tae-Hun; Cho, Hyun-Suk; Lee, Kijong;
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BACKGROUND: Genetically modified(GM) trigonal cactus(Hylocereus trigonus Saff.) contained a coat protein gene of cactus virus X (CVX), which conferred resistance to the virus, phosphinothricin acetyltransferase (bar) gene, which conferred herbicide resistance, and a cauliflower mosaic virus 35S promoter (CaMV 35S). This study was conducted to evaluate the possible impact of GM trigonal cactus cultivation on the soil microbial community. METHODS AND RESULTS: Microorganisms were isolated from the rhizosphere of GM and non-GM trigonal cactus cultivation soils. The total numbers of bacteria, and actinomycete in the rhizosphere soils cultivated GM and non-GM trigonal cactus were similar to each other, and there was no significant difference. Dominant bacterial phyla in the rhizosphere soils cultivated with GM and non-GM trigonal cactus were Proteobacteria, Uncultured archaeon, and Uncultured bacterium. The denaturing gradient gel electrophoresis (DGGE) profiles show a similar patterns, significant difference was not observed in each other. DNA was isolated from soil cultivated GM and non-GM trigonal cactus, we analyzed the persistence of the inserted gene by PCR. Amplification of the inserted genes was not observed in the soil DNA, which was collected after harvest. CONCLUSION(S): This result suggests that the GM trigonal cactus cultivation does not change significantly the microbial community.
CVX;Soil microbial community;Trigonal cactus;
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