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Effects of Protox Herbicide Tolerance Rice Cultivation on Microbial Community in Paddy Soil
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 Title & Authors
Effects of Protox Herbicide Tolerance Rice Cultivation on Microbial Community in Paddy Soil
Oh, Sung-Dug; Ahn, Byung-Ohg; Kim, Min-Kyeong; Sohn, Soo-In; Ryu, Tae-Hun; Cho, Hyun-Suk; Kim, Chang-Gi; Back, Kyoung-Whan; Lee, Kijong;
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BACKGROUND: Rice (Oryza sativa) is the most important staple food of over half the world's population. This study was conducted to evaluate the possible impact of transgenic rice cultivation on the soil microbial community. METHODS AND RESULTS: Microorganisms were isolated from the rhizosphere of GM and non-GM rice cultivation soils. Microbial community was identified based on the culture-dependent and molecular biology methods. The total numbers of bacteria, fungi, and actinomycete in the rhizosphere soils cultivated with GM and non-GM rice were similar to each other, and there was no significant difference between GM and non-GM rice. Dominant bacterial phyla in the rhizosphere soils cultivated with GM and non-GM rice were Actinobacteria, Firmicutes, and Proteobacteria. The microbial communities in GM and non-GM rice cultivated soils were characterized using the denaturing gradient gel electrophoresis (DGGE). The DGGE profiles showed similar patterns, but didn't show significant difference to each other. DNAs were isolated from soils cultivating GM and non-GM rice and analyzed for persistence of inserted gene in the soil by using PCR. The PCR analysis revealed that there were no amplified protox gene in soil DNA. CONCLUSION(S): These data suggest that transgenic rice does not have a significant impact on soil microbial communities, although continued research may be necessary.
GMO;Protox;Rice;Soil microbial community;
 Cited by
가뭄저항성 GM벼(Agb0103)와 non-GM 일미벼 간 표면미생물의 변화 및 병 발생 양상 비교,이승열;백창기;박상규;김경민;서상재;정희영;

한국육종학회지, 2016. vol.48. 2, pp.111-118 crossref(new window)
The GMO Industry: A Neglected Earthly Frontier, Journal of Hunger & Environmental Nutrition, 2016, 1  crossref(new windwow)
Bray, R.H., Kurtz, L.T., 1945. Determination of total, organic, and available forms of phosphorus in soils, Soil Sci. 59, 39-46. crossref(new window)

Brookes, G., Barfoot, P, 2006. Global impact of biotech crops: Socio-economic and environmental effects in the first ten years of commercial use, AgBioForum 9, 139-151.

Conner, A.J., Glare, T.R., Nap, J.P., 2003. The release of genetically modified crops into the environment; Part II. Overview of ecological risk assessment, Plant J. 33, 19-46. crossref(new window)

de Vries, J. Wackernagel, W., 2005. Microbial horizontal gene transfer and the DNA release from transgenic crop plants, Plant and Soil 266, 91-104. crossref(new window)

Ellstrand, N.C., 1992. Gene flow by pollen: Implications for plant conservation genetics, Oikos 63, 77-86. crossref(new window)

Filion, M., 2008. Do transgenic plants affect rhizobacteria populations?, Microb. Biotechnol. 1, 463-475. crossref(new window)

Germida, J.J., Dunfield, K.E., 2004. Impact of genetically modified crops on soil-and plant-associated microbial communities, J. Environ. Qual. 33, 806-815. crossref(new window)

James, C., 2011. Global Status of Commercialized Biotech/GM Crops: 2011, ISAAA Brief No. 43. ISAAA: Ithaca, NY.

Jonas, D.A., Elmadfa, I., Engel, K.H., Heller, K.J., Kozianowski, G., Konig, A., Muller, D., Narbonne, J.F., Wackernagel, W., Kleiner, J., 2001. Safety considerations of DNA in food, Annu. Nut. Metab. 45, 235-254. crossref(new window)

Jung, S., Lee, Y., YANG, K., Lee, S.B., Jang, S.M., Ha, S.B., BACK, K., 2004. Dual targeting of Myxococcus xanthus protoporphyrinogen oxidase into chloroplasts and mitochondria and high level oxyfluorfen resistance, Plant Cell Environ. 27, 1436-1446. crossref(new window)

Jung, B.G., Choi, J.W., Yoon, J.H., Kim, Y.H., Yun, E.S., 2001. Monitoring on chemical properties of bench marked upland soils in Korea, Korean J. Soil Sci. Fert. 34, 326-332.

Kardol, P., Bezemer, T.M., Van Der Putten, W.H., 2006. Temporal variation in plant-soil feedback controls succession. Ecol. Lett. 9, 1080-1088. crossref(new window)

Kim, M.C., Ahn, J.H., Shin, H.C., Kim, T., Ryu, T.H., Kim, D.H., Song, H.G., Lee, G.H., Kai, J.O., 2008. Molecular analysis of bacterial community structures in paddy soils for environmental risk assessment with two varieties of genetically modified rice, Iksan 483 and Milyang 204, J Microbiol. Biotech. 18, 207-218.

Kim S.E., Moon, J.S., Kim, J.K., Choi, W.S., Lee, S.H., Kim, S.U., 2010. Investigation of possible horizontal gene transfer from transgenic rice to soil microorganisms in paddy rice field, J. Microbiol. Biotechnol. 20, 187-192. crossref(new window)

Lee, B.K., Kim, C.G., Park, J.Y., Park, K.W., Yi, H.B., Harn, C.H., Kim, H.M., 2007. Assessment of the persistence of DNA in decomposing leaves of CMVP0-CP transgenic chili pepper in the field conditions, Korean J. Environ. Agric. 26, 319-324. crossref(new window)

Lee, K., Yi, B.-Y., Kim, K.-H., Kim, J.-B., Suh, S.-C., Woo, H.-J., Shin, K.-S., and Kweon, S.-J., 2011. Development of efficient transformation protocol for soybean (Glycine max L.) and characterization of transgene expression after Agrobacterium-mediated gene transfer, J. Korean Soc. Appl. Biol. Chem. 54, 37-45. crossref(new window)

Lorenz, M.G., Blum, S.A.E., Wackernagel, W., 1997. Mechanism of retarded DNA degradation and prokaryotic origin of DNases in nonsterile soils, Syst. Appl. Microbiol. 20, 513-521. crossref(new window)

Miethling, R., Wieland, G., Backhaus, H., Tebbe, C.C., 2000. Variation of microbial rhizosphere communities in response to crop species, soil origin, and inoculation with Sinorhizobium meliloti L33, Microbial. Ecol. 40, 43-56. crossref(new window)

NIAST, 2000. Methods of analysis of soil and plant, National Institute of Agricultural Science and Technology, Suwon, Korea.

Ochman, H, Lawrence, J.G., Grooisman, E.A., 2000. Lateral gene transfer and the nature of bacterial innovation, Nature. 405, 299-304. crossref(new window)

Owen, M.D.K., 2000. Current use of transgenic herbicide-resistant soybean and corn in the USA., Crop Prot. 19, 765-771. crossref(new window)

Sharma, S., Aneja, M.K., Mayer, J., Munch, J.C., Schloter, M., 2005. Characterization of bacterial community structure in rhizosphere soil of grain legumes, Microbial. Ecol. 49, 407-415. crossref(new window)

Smalla, K., Gebhard, F., 1999. Monitoring field releases of genetically modified sugar beets for persistence of transgenic plant DNA and horizontal gene transfer, Fems Microbiol. Ecol. 28, 261-272. crossref(new window)

Sohn, S.I., Oh, Y.J., Oh, S.D., Kim, M.K., Ryu, T.H., Lee, K.J., Suh, S.C., Baek, H.J., Park, J.S., 2010. Molecular analysis of microbial community in soils cultivating Bt Chinese cabbage, Korean J. Environ. Agric. 29, 293-299. crossref(new window)

Walkley, A., Black, I.A., 1934. An examination of the Degtjareff method for determining soil organic matter, and a proposed modification of the chromic acid titration method, Soil Sci. 37, 29-38. crossref(new window)

Widmer F., Seidler, R.J., Donegan, K.K., Reed, G.L., 1997. Quantification of transgenic plant marker gene persistence in the field, Mol. Ecol. 6, 1-7. crossref(new window)