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Responses of Misgurnus anguillicaudatus and Cyprinus carpio Fed on Disease Resistant(OsCK1) Rice Variety
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 Title & Authors
Responses of Misgurnus anguillicaudatus and Cyprinus carpio Fed on Disease Resistant(OsCK1) Rice Variety
Oh, Sung-Dug; Lee, Kijong; Park, Soo-Yun; Lee, Dae-Yong; Sohn, Soo-In; Kim, Min-Young; Ryu, Tae-Hun;
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BACKGROUND: The disease resistant (OsCK1) rice was generated by inserting choline kinase (CK1) and phosphinothricin acetyltransferase (PAT) genes isolated from Oriza sativa and Streptomyces hygroscopicus into the genome of rice (Nakdongbyeo). With the potential problems of safety, the non-target organism evaluation is required as an essential element for the environmental risk assessment of genetically modified (GM) crops. In present study, we studied the effects on survival of Misgurnus anguillicaudatus and Cyprinus carpio, commonly used as a model organism in ecotoxicological studies. METHODS AND RESULTS: The M. anguillicaudatus and C. carpio were fed on disease resistant (OsCK1) rice and non-genetically modified (non-GM) rice (Nakdongbyeo) to 0, 10, 100, 1,000 and 5,000 mg/L, as treatment concentration respectively. The OsCK1 rice used for the test was confirmed to have the OsCK1/PAT gene expression by the PCR and ELISA analysis. Feeding test showed that no significant differences in cumulative immobility and abnormal response of M. anguillicaudatus and C. carpio fed on between OsCK1 rice and non-GM rice. The 96hr- values showed no difference between OsCK1 rice (>5,000 mg/L) and non-GM rice (>5,000 mg/L). CONCLUSION(S): The results of this study suggested that there was no significant difference in toxicity for M. anguillicaudatus and C. carpio between OsCK1 rice and non-GM counterparts.
Cyprinus carpio;Disease resistant transgenic rice;Misgurnus anguillicaudatus;Risk assessment;
 Cited by
병저항성 GM(OsCK1)벼의 물벼룩(Daphnia magna)에 대한 급성독성 평가,오성덕;이기종;박수윤;류태훈;서상재;

한국환경농학회지, 2014. vol.33. 2, pp.121-128 crossref(new window)
GM벼 OsCK1의 도입유전자 구조분석: Whole Genome Shotgun Sequencing을 이용한 다중 도입된 T-DNA 구조 분석,안홍일;이진형;친양;우희종;신공식;권순종;임명호;

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