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Study of Kidney Toxicity of Azadirachta Indica Extract for Oral Administration in Rats
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 Title & Authors
Study of Kidney Toxicity of Azadirachta Indica Extract for Oral Administration in Rats
Yoon, Hyunjoo; Choe, Miseon; Cho, Hyeon-Jo; Han, Beom Seok; Park, Kyung-Hun; Oh, Jin-Ah; Cho, Namjun; Paik, Min-Kyoung;
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BACKGROUND: Azadirachta indica has been widely used as environment-friendly organic materials because of its insecticidal properties. This study was carried out to investigate the acute toxicity and the subacute toxicity of Azadirachta indica extract(AIE) in rats. METHODS AND RESULTS: For the oral acute toxicity test, Sprague-Dawley rats were gavaged with 2.0 g/Kg bw of AIE. The value was greater than 2.0 g/Kg bw for both male and female rats. For the subacute toxicity study, rats were treated with AIE at doses of 0.5, 1.0, 2.0 mg/Kg bw once a day for 4 weeks(n=10 animals per each group). There were no significant changes in body weight, food intake and water consumption observed during the experimental duration. In addition, no difference of relative kidney weight was observed among all treated groups. Serum creatinine level in the AIE 2.0 g/Kg group increased significantly compared with that of control group in male rats, but serum blood urea nitrogen was significantly decreased in a dose-dependent manner (p<0.05). Significant increase of serum cholesterol levels were observed in all AIE groups, compared with the control group, in the female rats (p<0.05). However, histopathological examination of the kidney did not reveal any significant lesions in all groups. CONCLUSION: On the basis of results, it could be concluded that oral administration AIE didn't cause any toxic response in kidney, except the increased serum cholesterol.
Azadirachta indica extract;Kidney;Subacute toxicity;
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수용성 님추출물이 랫드의 간 독성에 미치는 영향,박경훈;윤현주;한범석;이제봉;정미혜;조남준;엄애선;백민경;

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Abdel Megeed, M.I., Radwan, U.M., Hindy, A.Z., El Zarook, A., 2001. Liver functions under stress of certain common pesticides residue used on fruits and vegetables orally administrated, Annals Agric. Sci. 46, 383-404.

Alam, M.M., Siddiqui, M.B., Husain, W., 1990. Treatment of diabetes through herbal drug in rural India, Fitoterapia 61, 240-242.

Ashafa, A.O.T., Orekoya, L.O., Yakuba, M.T., 2012. Toxicity profile of ethanolic extract of Azadirachta indica stem bark in male Wistar rats, Asian Pac. J. Trop. Biomed. 2, 811-817. crossref(new window)

Bandyopadhyay, U., Biswas, K., Sengupta, A., Moitra, P., Dutta, P., Sarkar, D., Debnath, P., Ganguly, C.K., Banerjee, R.K., 2004. Clinical studies on the effect of Neem (Azadirachta indica) bark extract on gastric secretion and gastroduodenal ulcer, Life Sci. 75, 2867-2878. crossref(new window)

Biswas, K., Chattopadhyay, I., Banerjee, R. K., Bandyopadhyay, U., 2002. Biological activities and medicinal properties of neem (Azadirachta indica), Indian Acad. Sci. 82, 1336-1345.

Kaysan, G.A., 2007. Hyperlipidemia in chronic kidney disease, Int. J. Artif. Organs 30, 987-992. crossref(new window)

Cheesbrough, M., 1991. Medical Laboratory Manual for Tropical Countries, pp. 133-160, 2nd ed. University Press, Cambridge, Great Britain.

Choi, H. S., Wu, X. Y., Kim, W. S., Lee, Y., Choi, B. M., Kuk, Y. I., 2011. Effects of organic materials on insect and disease occurrence and fruit quality in pear orchards, Korean J. Org. Agric. 19, 405-416.

Das, B.K., Mukherjee, S.C., Murjani, O., 2002. Acute toxicity of neem (Azadirachta indica) in Indian major carps, J. Aquac. Trop. 17, 23-33.

Das, B.K., Mukherjee, S.C., Sahu, B.B., Murjani, G., 1999. Neem (Azadirachta indica) extract as an antibacterial agent against fish pathogenic bacteria, Indian J. Exp. Biol. 37, 1097-1100.

Deng, Y.X., Cao, M., Shi, D.X., Yin, Z.Q., Jia, R.Y., Xu, J., Wang, C., Lv, C., Liang, X.X., He, C.L., Yang, Z.R., Zhao, J., 2013. Toxicological evaluation of neem (Azadirachta indica) oil: acute and subacute toxicity, Environ. Toxicol. Pharmacol. 35, 240-246. crossref(new window)

Dhaliwal, P.K., Roop, J.K., Guraya, S.S., Dhawan, A.K., 1998. Antifertility activity of neem-seed oil in cyclic female rats, in: Dhaliwal, P.K., Randhawa, N.S., Arora, A. and Dhawan, A.K. (Eds), Ecological agriculture and sustainable development, Center for Research in Rural & Industrial Development, Ludhiana, India. pp. 340-346.

EFSA (European Food Safety Authority), 2011. Conclusion on the peer review of the pesticide risk assessment of the active substance azadirachtin, EFSA J. 9, 1858. crossref(new window)

Ezz-Din, D., Gabry, M.S., Farrag, A.R.H., Abdel Moneim, A.E., 2011. Physiological and histological impact of Azadirachta indica (neem) leaves extract in a rat model of cisplatin-induced hepato and nephrotoxicity, J. Med. Plants Res. 5, 5499-5506.

Gandhi, M., Lal, R., Sankaranarayanan, A., Banerjee, C.K., Sharma, P.L., 1988. Acute toxicity study of the oil from Azadirachta indica seed (neem oil), J. Ethnopharmcol. 23, 39-51. crossref(new window)

Garg, S., Taluja, V., Upadhyay, S.N., Talwar, G.P., 1993. Studies on the contraceptive efficacy of praneem polyherbal cream, Contraception. 48, 596-591.

Han, S.B., Kim, J.H., 2013. Research trend of neem based biopesticides, Korean J. Pestic. Sci. 17, 220-230. crossref(new window)

Koner, B.C., Banerjee, B.D., Ray, A., 1997. Effects of stress on gamma glutamyl transpeptidase (GGT) activity in lymphoid system of rats: modulation by drugs, Indian J. Exp. Biol. 35, 222-224.

Kumar, G.H., Priyadarsini, R.V., Vinothini, G., Letchoumy, P.V., Nagini, S., 2010. The neem limonoids azadirachtin and nimbolide inhibit cell proliferation and induce apoptosis in an animal model of oral oncogenesis, Invest New Drugs. 4, 392-401.

Lee, J.W., Jin, C.L., Jang, K.C., Choi, G.H., Lee, D., Kim, J.H., 2013. Investigation on the insecticidal limonoid content of commercial biopesticides and neem extract using solid phase extraction, J. Agric. Chem. Environ. 2, 81-85.

Parshad, O., Singh, P., Gardner, M., Fletcher, C., Rickards, E., Choo-Kang, E., 1994. Effect of aqueous neem (Azadirachta indica) extract on testosterone and other blood constituents in male rats. A pilot study, West Indian Med. J. 43, 71-74.

Rahman, M.F., Siddiqui, M.K.J., Jamil, K., 2001. Effects of vepacide (Azadirachta indica) on aspartate and alanine aminotransferase profiles in a subchronic study with rats, Hum. Exp. Toxicol. 20, 243-249. crossref(new window)

Rahman, M.F., Siddiqui, M.K.J., Jamil, K., 2002. LDH profiles of male and female rats treated with vepacide, Phytother. Res. 16, 122-126. crossref(new window)

Raizada, R.B., Srivastava, M.K., Kaushal, R.A., Singh, R.P., 2001. Azadirachtin, a neem biopesticide: subchronic toxicity assessment in rats. Food and Chemical Toxicology 39, 477-483. crossref(new window)

Ray, A., Banerjee, B.D., Sen, P., 1996. Modulation of humoral and cell-mediated immune responses by Azadirachta indica (Neem) in mice, Indian J. Exp. Biol. 34, 698-701.

Selim, M.E., Yousef, O.M., Hamid, S.H., Aleisa, N.A., 2013. Hyperlipidemia aggravated renal disease in bacteremic male albino rats, J. Med. Sci. 1, 9-22.

Singh, P.P., Junnarkar, A.Y., Thomas, G.P., Tripathi, R.M., Varma, R.K., 1990. A pharmacological study of Azadirachta indica, Fitoterapia 61, 164-168.