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Acute Toxicity Evaluation to Daphnia magna of Disease Resistant(OsCK1) Rice
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 Title & Authors
Acute Toxicity Evaluation to Daphnia magna of Disease Resistant(OsCK1) Rice
Oh, Sung-Dug; Lee, Kijong; Park, Soo-Yun; Ryu, Tae-Hun; Suh, Sang Jae;
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 Abstract
BACKGROUND: The disease resistant (OsCK1) rice was generated by inserting choline kinase (CK1) and phosphinothricin acetyltransferase (PAT) genes isolated from Oryza sativa and Streptomyces hygroscopicus into the genome of the rice, Nakdongbyeo. With the potential problems of safeties, the evaluations on non-target organisms are essentially required for the environmental risk assessment of genetically modified (GM) crops. In the present study, we conducted the evaluation of acute toxicity on Daphnia magna that commonly used as a model organism in ecotoxicological studies for non-target organism evaluation. METHODS AND RESULTS: Effect of acute toxicity to Daphnia magna by each concentration were investigated in the disease resistant (OsCK1) rice and non-genetically modified (non-GM) rice, Nakdongbyeo, as concentration (0, 1,000, 1,800, 3,240, 5,830, 10,500 and 20,000 mg/L). The OsCK1 rice used for the test was confirmed to express the OsCK1/PAT gene by the PCR(Polymerase chain reaction) and western blot analysis. Feeding test showed that no significant differences in cumulative immobility and abnormal response of Daphnia magna fed on OsCK1 rice or non-GM rice. The 48hr- values showed no difference between OsCK1 rice (3,147.18 mg/L) and non-GM rice (3,596.27 mg/L). CONCLUSION: This result suggested that there was no significant difference in toxicity to Daphnia magna between OsCK1 rice and non-GM counterpart.
 Keywords
Daphnia magna;Disease resistant transgenic rice;Risk assessment;
 Language
Korean
 Cited by
1.
Acute toxicity evaluation of drought-tolerant transgenic rice Agb0103 to Daphnia magna, Korean Journal of Agricultural Science, 2016, 43, 2, 205  crossref(new windwow)
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