Publisher : The Korean Society of Environmental Agriculture
DOI : 10.5338/KJEA.2014.33.2.129
Title & Authors
Selection and Isolation of a Mutant Yeast Strain Tolerant to Multiple Targeted Heavy Metals Lee, Sangman;
BACKGROUND: This study was performed for selecting yeast mutants with a high tolerance for targeted metals, and determining whether yeasts strains tolerant to multiple heavy metals could be induced by sequential adaptations. METHODS AND RESULTS: A mutant yeast strain tolerant to the heavy metals cadmium (Cd), copper (Cu), nickel (Ni), and zinc (Zn) was selected by sequential elevated exposures to each metal with intermittent mutant isolation steps. A Cd-tolerant mutant was isolated by growing yeast cells in media containing concentrations that were gradually increased to 1 mM. Then the Cd-tolerant mutant was gradually exposed to increasing levels of in growth media until a concentration of 7 mM was reached, thus generating a strain tolerant to both Cd and Cu. In the subsequent steps, this mutant was exposed to (up to 8 mM), and a resultant isolate was further exposed to (up to 60 mM), allowing the derivation of a yeast mutant that was simultaneously tolerant to Cd, Cu, Ni, and Zn. CONCLUSION: This method of inducing tolerance to multiple targeted heavy metals in yeast will be useful in the bioremediation of heavy metals.
Diaz-Ravina, M., Baath, E., 1996. Development of metal tolerance in soil bacterial communities exposed to experimentally increased metal levels, Appl. Environ. Microbiol. 62, 2970-2977.
Diaz-Ravina, M., Baath, E., 2001. Response of bacterial communities pre-exposed to different metals and reinoculated in an unpolluted soil, Soil Biol. Biochem. 33, 241-248.
Duxbury, T., Bicknell, B., 1983. Metal-tolerant bacterial populations from natural and metal-polluted soils, Soil Biol. Biochem. 15, 243-250.
Gad, A.S., Attia, M., Ahmed, H.A., 2010. Heavy metals bio-remediation by immobilized Saccharomyces cerevisiae and Opuntia ficus indica waste, J. American Sci. 6, 79-87.
Gaur, N., Flora, G., Yadav, M., Tiwari, A., 2014. A review with recent advancements on bioremediationbased abolition of heavy metals, Environ. Sci. Processes Impacts 16, 180-193.
Giaginis, C., Gatzidou, E., Theocharis, S., 2006. DNA repair systems at targets of cadmium toxicity, Toxicol..l Appl. Pharmacol. 213, 282-290.
Gin, Y.H., Clark, A.B., Slebos, R.J., Al-Rafai, H., Taylor, J.A., Kundel, T.A., Resnick, M.A., Gordenin, A., 2003. Cadmium is a mutagen that acts by inhibiting mismatch repair, Nat. Gen. 34, 326-329.
Lee, S., Kim, J.H., 2010. Establishment of tolerance to both cadmium and copper stress by expressing Arabidopsis phytochelatin synthase in Cu tolerant yeast mutant, J. Korean Soc. Appl. Biol. Chem. 53, 94-96.
Rehman, A., Farooq, H., Hasnain, S., 2008. Biosorption of copper by yeast, Loddermyces elongisporus, isolated from industrial effluents: its potential use in wastewater treatment, J. Basic Microbiol. 48, 195-201.
Ruta, L., Paraschivescu, C., Matache, M., Avramescu, S., Farcassanu, I.C. 2010. Removing heavy metals from synthetic effluents using "kamikaze" Saccharomyces cerevisiae cells, Appl. Microbiol. Biotechnol. 85, 763-771.
Serero, A., Lopes, J., Nicolas, A., Boiteux, S., 2008. Yeast genes involved in cadmium tolerance: Identification of DNA replication as a target of cadmium toxicity, DNA Repair 7, 1262-1275.
Soares, E.V., Soares, H.M., 2012. Bioremediation of industrial effluents containing heavy metals using brewing cells of Saccharomyces cerevisiae as a green technology: a review, Environ. Sci. Pollut. Res. Int. 19, 1066-1083.
Villegas, L.B., Amoroso, M.J., deFigueroa, L.I.C., 2005. Copper tolerant yeasts isolated from polluted area of Argentina, J. Basic Microbiol. 45, 381-391.
Zafar, S., Aquil, F., Ahmad, I., 2007. Metal tolerance and biosorption potential of filamentous fungi isolated from metal contaminated agricultural soil, Bioresource Technol. 98, 2557-2561.