Publisher : The Korean Society of Environmental Agriculture
DOI : 10.5338/KJEA.2014.33.2.138
Title & Authors
Genotoxicity of Environment-friendly Organic Materials of Plant Origin in the Micronucleus Test Using Chinese Hamster Lung Cells Cho, Hyeon-Jo; Park, Kyung-Hun; Jeong, Mi Hye; Park, Soo Jin; Oh, Jin-Ah; Kim, Won-Il; Cho, Namjun; Ryu, Jae-Gee; Paik, Min-Kyoung;
BACKGROUND: Azadirachta Indica extract(AIE) and Sophorae radix extract(SRE) are widely used as environment-friendly organic materials of plant origin in South Korea. METHODS AND RESULTS: In this study, the in vitro micronucleus(vitMN) tests of two samples of AIE and SRE were conducted to evaluate their genotoxicity using the Chinese hamster lung(CHL) cell. This study was composed of two parts; cytochalasin B(cyto B) test and non-cyto B test. Mitomycin C and colchicine were used as positive controls. As a result, the incidence of micronucleus(MN) in all AIE and SRE treated groups increased in dose-dependent manner, but were less than 2.2% in 1,000 binucleated cells. In addition, there were no significant increases of MN incidence in all AIE and SRE treated groups, compared with the negative control group. CONCLUSION: Therefore, we suggest that AIE samples and SRE samples used in this study may have no genotoxicity in the in vitro micronucleus test using the CHL cells. In our previous study, we reported that AIE and SRE did not cause genotoxicity in Ames test. According to the genotoxicity battery system, we concluded that AIE and SRE used in this study have no genotoxic effects to humans.
Corvi, R., Albertini, S., Hartung, T., Hoffmann, S., Maurici, D., Pfuhler, S., van Benthem, J., Vanparys, P., 2008. ECVAM retrospective validation of in vitro micronucleus test (MNT), Mutagenesis 23, 271-283.
Cho, H.J., Yoon, H.J., Park, K.H., Lee, J.B., Kim, C.K., Kim, J.H., Jeong, M.H., Oh, J.A., Kim, D.H., Paik, M.K., 2013. In vitro Antimutagenic and Genotoxic Effects of Sophora Radix Extracts. Korean J. Pestic. Sci. 17, 335-342.
Fenech, M. Chang, W.P. Kirsch-Volders, M. Holland, N. Bonassi, S., Zeiger, E. 2003. HUMN project: detailed description of the scoring criteria for the cytokinesisblock micronucleus assay using isolated human lymphocyte cultures. Mutation Research 534, 65-75.
Garriott, M.L., Phelps J.B., Hoffman W.P., 2002. A protocol for the in vitro micronucleus test I. Contributions to the development of a protocol suitable for regulatory submissions from an examination of 16 chemicals with different mechanisms of action and different levels of activity. Mutation Research 517, 123-134.
Lilienblum, W., Dekant, W., Foth, H., Gebel, T., Hengstler, J.G., Kahl, R., Kramer, P.J., Schweinfurth, H., Wollin, K.M., 2008. Alternative methods to safety studies in experimental animals: role in the risk assessment of chemicals under the new European Chemicals Legislation (REACH). Arch.Toxicol. 82, 211-236.
Miller, B.M., Pujadas, E., Gocke, E., 1995. Evaluation of the micronucleus test in vitro using Chinese hamster cells: results of four chemicals weakly positive in the in vivo micronucleus test. Environ. Mol. Mutagen. 26, 240-247.
Wang, X., Liang, L., Chang, J., Yang, M., Li, Z., 2010. Toxicity of matrine in Kunming mice. J. South Med. Univ. 30, 2154-2155.
Woo, H.D., Lee, J.K., Han, G.D., 2010. Consumer awareness survey on safety management of pesticide residue. Food Sci. Industry 43, 24-40.
Yoon, H.J., Cho, H.J., Lee, J.B., Kim, J.H., Park, K.H., Oh, J.A., Kim, D.H., Paik, M.K., 2014 In vitro antimutagenic and genotoxic Effects of Azadirachta indica Extract. J. Appl. Biol. Chem. http://dx.doi.org/10.3839/jabc.2014.035.