Advanced SearchSearch Tips
Phytoextraction of Heavy Metals Induced by Bioaugmentation of a Phosphate Solubilizing Bacterium
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
Phytoextraction of Heavy Metals Induced by Bioaugmentation of a Phosphate Solubilizing Bacterium
Arunakumara, K.K.I.U.; Walpola, Buddhi Charana; Song, Jun-Seob; Shin, Min-Jung; Lee, Chan-Jung; Yoon, Min-Ho;
  PDF(new window)
BACKGROUND: Excessive metals in the soil have become one of the most significant environmental problems. Phytoremediation has received considerable attention as a method for restoring the contaminated soils. The microbes having remarkable metal tolerance and plant growth-promoting abilities could also play a significant role in remediation of metal-contaminated soils, because bioaugmentation with such microbes could promote phytoextraction of metals. Therefore, the present study was focused on evaluating the phytoextraction of heavy metals (Co, Pb and Zn) in Helianthus annuus (sunflower) induced by bioaugmentation of a phosphate solubilizing bacterium. METHODS AND RESULTS: A phosphate solubilizing bacterium was isolated from metal-contaminated soils based on the greater halo size (>3 mm) with solid NBRIP agar medium containing 10 g glucose, 5 g , 5 g , 0.25 g , 0.2 g KCl, 0.1 g in 1 L distilled water. Isolated bacterial strain was assessed for their resistance to heavy metals; , , and at various concentrations ranging from (Co, Pb and Zn) using the agar dilution method. A pot experiment was conducted with aqueous solutions of different heavy metals (Co, Pb and Zn) to assess the effect of bacterial strain on growth and metal uptake by Helianthus annuus (sunflower). The impact of bacterial inoculation on the mobility of metals in soil was investigated under laboratory conditions with 50 mL scaled polypropylene centrifuge tubes. The metal contents in the filtrate of plant extracts were determined using an atomic absorption spectrophotometer (Perkinelmer, Aanalyst 800, USA). CONCLUSION: Inoculation with Enterobacter ludwigii PSB 28 resulted in increased shoot and root biomass and enhanced accumulation of Co, Pb and Zn in Helianthus annuus plants. The strain was found to be capable of promoting metal translocation from the roots to the shoots of H. annuus. Therefore, Enterobacter ludwigii PSB 28 could be identified as an effective promoter of phytoextraction of Co, Pb and Zn from metal-contaminated soils.
Enterobacter ludwigii PSB 28;Inoculation;Phytoextraction;Sunflower;
 Cited by
Value added phytoremediation of metal stressed soils using phosphate solubilizing microbial consortium, World Journal of Microbiology and Biotechnology, 2017, 33, 1  crossref(new windwow)
Abou-Shanab, R.A.I., Angle, J.S., Chaney, R.L. 2006. Bacterial inoculants affecting nickel uptake by Alyssum murale from low, moderate and high Ni soils, Soil Biol. Biochem. 38, 2882-2889. crossref(new window)

Abou-Shanab, R.A., van Berkum, P., Angle, J.S. 2007. Heavy metal resistance and genotypic analysis of metal resistance genes in gram-positive and gram-negative bacteria present in Ni-rich serpentine soil and in the rhizosphere of Alyssum murale, Chemosphere 68, 360-367. crossref(new window)

Arunakumara, K.K.I.U. 2011. Use of Crop Plants for Removal of Toxic Metals, in: Khan MS, Zaidi A, Goel R, Mussarrat J. (Eds), Bio-management of Metal Contaminated Soils, Springer, Netherlands, pp. 439-457.

Arunakumara, K.K.I.U., Walpola, B.C., Yoon, M.H. 2013. Banana peel: A green solution for metal removal from contaminated waters, Korean J. Environ. Agric. 32, 108-116. crossref(new window)

Arunakumara, K.K.I.U., Walpola, B.C., Yoon, M.H. 2013. Agricultural methods for toxicity alleviation in metal contaminated soils, Korean J. Soil Sci. Fert. 46, 73-80. crossref(new window)

Baum, C., Hrynkiewicz, K., Leinweber, P., Meissner, R. 2006. Heavy-metal mobilization and uptake by mycorrhizal and nonmycorrhizal willows (Salix dasyclados), J. Plant Nutr. Soil Sci. 169, 516-522. crossref(new window)

Belimov, A.A., Hontzeas, N., Safronova, V.I., Demchinskaya, S.V., Piluzza, G., Bullitta, S., Glick, B.R. 2005. Cadmium-tolerant plant growth-promoting bacteria associated with the roots of Indian mustard (Brassica juncea L. Czern.), Soil Biol. Biochem. 37, 241-250. crossref(new window)

Belimov, A.A., Kunakova, A.M., Safronova, V.I., Stepanok, V.V., Yudkin, L.Y., Alekseev, Y.V., Kozhemyakov, A.P. 2004. Employment of rhizobacteria for the inoculation of barley plants cultivated in soil contaminated with lead and cadmium, Microbiology 73, 99-106. crossref(new window)

Belimov, A.A., Safronova, V.I., Sergeyeva, T.A., Egorova, T.N., Matveyeva, V.A., Tsyganov, V.E., Borisov, A.Y., Tikhonovich, I.A., Kluge, C., Preisfeld, A., Dietz, K.J., Stepanok, K.J. 2001. Characterisation of plant growthpromoting rhizobacteria isolated from polluted soils and containing 1-aminocyclopropane-1-carboxylate deaminase, Canadian J. Microbiol. 47, 642-652. crossref(new window)

Boonyapookana, B., Parkpian, P., Techapinyawat, S., DeLaun, R.D., Jugsujinda, A. 2005. Phytoaccumulation of lead by sunflower (Helianthus annuus), tobacco (Nicotiana tabacum), and vetiver (Vetiveria zizanioides), J. Environ. Sci. Health, Part A. 40, 117-137. crossref(new window)

Borgmann, U. 2000. Methods for assessing the toxicological significance of metals in aquatic ecosystems: bio-accumulation-toxicity relationships, water concentrations and sediment spiking approaches, Aquatic Ecosyst Health Manag. 3, 277-289.

Braud, A., Jezequel, K., Vieille, E., Tritter, A., Lebeau, T. 2006. Changes in extractability of Cr and Pb in a polycontaminated soil after bioaugmentation with microbial producers of biosurfactants, organic acids and siderophores, Water Air Soil Pollut. : Focus 6, 261-279. crossref(new window)

Cao, A., Carucci, A., Lai, T., La Colla, P., Tamburini, E. 2007. Effect of biodegradable chelating agents on heavy metals phytoextraction with Mirabilis jalapa and on its associated bacteria. Euro. J Soil Biol. 43, 200-206. crossref(new window)

Cervantes-Vega, C., Chavez, J., Cordova, N.A., de la Mora, P., J. 1986. Resistance to metal by Pseudomonas aeruginosa clinical isolates, Microbios 48, 159-163.

Chen, B., Shen, H., Li, X., Feng, G., Christie, P. 2004. Effects of EDTA application and arbuscular mycorrhizal colonization on growth and zinc uptake by maize (Zea mays L.) in soil experimentally contaminated with zinc, Plant Soil 261, 219-229. crossref(new window)

Chen, Y.E., Yuan, S., Su, Y.Q., Wang, L. 2010. Comparison of heavy metal accumulation capacity of some indigenous mosses in Southwest China cities: a case study in Chengdu city, Plant Soil Environ. 56, 60-66.

Chen, Y.X., Wang, Y.P., Lin, Q., Luo, Y.M. 2005. Effect of copper-tolerant rhizosphere bacteria on mobility of copper in soil and copper accumulation by Elsholtzia splendens, Environ. Int. 31, 861-866. crossref(new window)

Citterio, S., Prato, N., Fumagalli, P., Aina, R., Massa, N., Santagostino, A., Sgorbati, S., Berta, G. 2005. The arbuscular mycorrhizal fungus Glomus mosseae induces growth and metal accumulation changes in Cannabis sativa L, Chemosphere 59, 21-29. crossref(new window)

Di Gregorio, S., Barbafieri, M., Lampis, S., Sanangelantoni, A.M., Tassi, E., Vallini, G. 2006. Combined application of Triton X-100 and Sinorhizobium sp. Pb002 inoculum for the improvement of lead phytoextraction by Brassica juncea in EDTA amended soil, Chemosphere 63, 293-299. crossref(new window)

Egamberdiyeva, D., Juraeva, D., Gafurova, L., Hoflich, G. 2002. Promotion of plant growth of maize by plant growth promoting bacteria in different temperature and soils. In: van Santen, E. (Eds), Making Conservation Tillage Conventional: Building a Future on 25 Years of Research. Proceedings of 25th Annual Southern Conservation Tillage Conference for Sustainable Agriculture. Auburn, AL 24-26 June 2002. Special Report No. 1. Alabama Agricultural Experiment Station and Auburn University, AL 36849, USA.

El-Tayeb, M.A., El-Enany, A.E., Ahmed, N.L. 2006. Salicylic acid-induced adaptive response to copper stress in sunflower (Helianthus annuus L.), Plant Growth Regul. 50, 191-199. crossref(new window)

Fazal, H., Bano, A. 2010. The effect of diazotrophs (rhizobium and azatobactor) on growth and biomass of maize in lead (Pb) polluted soil, and accumulation of the lead in different parts of plant, P. J. Bot. 42, 4363-4370.

Freitas, H., Prasad, M.N.V., Pratas, J. 2004. Analysis of serpentinophytes from north-east of Portugal for trace metal accumulation-relevance to the management of mine environment, Chemosphere 54, 1625-1642. crossref(new window)

Gadd, G.M. 2004. Microbial influence on metal mobility and application for bioremediation, Geoderma 122, 109-119. crossref(new window)

Hemambika, B., Balasubramanian, V., Kannan, V.R., James, R.A. 2013. Screening of chromium-resistant bacteria for plant growth-promoting activities, Soil Sediment Contam. 22, 717-736. crossref(new window)

Jiang, C.Y., Sheng, X.F., Qian, M., Wang, Q.Y. 2008. Isolation and characterization of a heavy metal-resistant Burkholderia sp. from heavy metal-contaminated paddy field soil and its potential in promoting plant growth and heavy metal accumulation in metal-polluted soil, Chemosphere 72, 157-164. crossref(new window)

Jing, Y.D., He, Z.L., Yang, X.E. 2007. Role of soil rhizobacteria in phytoremediation of heavy metal contaminated soils, J. Zhejiang Univ. Sci. B 8, 192-207.

Karavoltsos, S., Sakellari, A., Dimopoulos, M., Dassenakis, M., Scoullos, M. 2002. Cadmium content in foodstuffs from the Greek market, Food Addit. Contam. 19, 954-962. crossref(new window)

Kayser, G., Korckritz, T., Markert, B. 2001. Bioleaching for the decontamination of heavy metals, Wasser Boden. 53, 54-58.

Kloepper, J.W. 2003. A review of mechanisms for plant growth promotion by PGPR, in: Sixth International PGPRWorkshop, Calicut, India, pp. 81-92.

Kumar, S., Tamura, K., Jakobsen, I.B., Nei, M. 2001. MEGA2: molecular evolutionary genetics analysis software, Bioinformatics 17, 1244-1245. crossref(new window)

Lebeau, T., Braud, A., Jezequel, K. 2008. Performance of bioaugmentation-assisted phytoextraction applied to metal contaminated soils: A review, Environ. Pollut. 153, 497-522. crossref(new window)

Luo, L., Ma, Y., Zhang, S., Wei, D., Zhu, Y.G. 2009. An inventory of trace element inputs to agricultural soils in China, J. Environ. Manage. 90, 2524-2530. crossref(new window)

Marchiol, L., Fellet, G., Perosa, D., Zerbi, G. 2007. Removal of trace metals by Sorghum bicolor and Helianthus annuus in a site polluted by industrial wastes: a field experience, Plant Physiol. Biochem. 45, 379-387. crossref(new window)

McNear Jr, D.H. 2013. The rhizosphere-roots, soil and everything in between, Nature Education Knowledge 4, 1.

Nautiyal CS. 1999. An efficient microbiological growth medium for screening of phosphate solubilizing microorganisms, FEMS Microbiol. Lett. 170, 265-270. crossref(new window)

Ouzounidou, G., Ilias, I. 2005. Hormone-induced protection of sunflower photosynthetic apparatus against copper toxicity, Biol. Plantarum.49, 223-228. crossref(new window)

Pal, A., Dutta, S., Mukherjee, P.K., Paul, A.K. 2005. Occurrence of heavy metal resistance in microflora from serpentine soil of Andaman, J. Basic. Microbiol. 45, 207-218. crossref(new window)

Prapagdee, B., Chumphonwong, N., Khonsue, N., Mongkolsuk, S., 2012. Influence of cadmium resistant bacteria on promoting plant root elongation and increasing cadmium mobilization in contaminated soil, Fresenius Environmental Bulletin 21, 1186-1191.

Prapagdee, B., Chanprasert, M., Mongkolsuk, S., 2013. Bioaugmentation with cadmium-resistant plant growth-promoting rhizobacteria to assist cadmium phytoextraction by Helianthus annuus, Chemosphere 92, 659-666. crossref(new window)

Rajkumar, M., Ying, M., Freitas, H., 2008. Characterization of metal-resistant plant-growth promoting Bacillus weihenstephanensis isolated from serpentine soil in Portugal, J. basic microbial. 48, 500-508. crossref(new window)

Ryan, P.R., Dessaux, Y., Thomashow, L.S., Weller, D.M., 2009. Rhizosphere engineering and management for sustainable agriculture, Plant Soil 321, 363-383. crossref(new window)

Saitou, N., Nei, M., 1987. The neighbor-joining method: a new method for reconstructing phylogenetic trees, Mol. Biol. Evol. 4,406-425.

Sheng X.F., Xia, J.J., 2006. Improvement of rape (Brassica napus) plant growth and cadmium uptake by cadmium-resistant bacteria, Chemosphere 64, 1036-1042. crossref(new window)

Singh, S., Aggarwal, P.K., 2006. Effect of heavy metals on biomass and yield of different crop species, Indian J. Agric. Sci. 76, 688-691.

Thomas, E.Y., Omueti, J.A.I., Ogundayomi, O., 2012. The effect of phosphate fertilizer on heavy metal in soils and Amaranthus Caudatu, Agric. Biol. J. N. Am. 3, 145-149. crossref(new window)

Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F., Higgins, D. G., 1997. The Clustal X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools, Nucl. Acids Res. 25, 4876-4882. crossref(new window)

Turgut C., Katie Pepe, M., Cutright, T.J., 2004. The effect of EDTA and citric acid on phytoremediation of Cd, Cr, and Ni from soil using Helianthus annuus, Environ. Pollut. 131, 147-154. crossref(new window)

Wani, P.A., Khan, M.S., Almas, Z. 2007. Synergistic effects of the inoculation with nitrogen-fixing and phosphate-solubilizing rhizobacteria on the performance of field-grown chickpea. J Plant Nutr Soil Sci. 170, 283-287. crossref(new window)

Wang, Y.P., Shi, J.Y., Lin, Q., Chen, X.C., Chen, Y.X., 2007. Heavy metal availability and impact on activity of soil microorganisms along a Cu/Zn contamination gradient, J. Environ. Sci. 19, 848-853. crossref(new window)

Whiting S. N., de Souza, M. P., Terry, N., 2001. Rhizosphere Bacteria Mobilize Zn for Hyperaccumulation by Thlaspicaerulescens, Environ. Sci. Technol. 35, 3144-3150. crossref(new window)

Zaidi S., Usmani, S., Singh B.R., Musarrat, J., 2006. Significance of Bacillus subtilis strain SJ-101 as a bioinoculant for concurrent plant growth promotion and nickel accumulation in Brassica juncea, Chemosphere 64, 991-997. crossref(new window)