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Effective Suppression of Methane Production by Chelating Nickel of Methanogenesis Cofactor in Flooded Soil Conditions
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 Title & Authors
Effective Suppression of Methane Production by Chelating Nickel of Methanogenesis Cofactor in Flooded Soil Conditions
Kim, Tae Jin; Hwang, Hyun Young; Hong, Chang Oh; Lee, Jeung Joo; Kim, Gun Yeob; Kim, Pil Joo;
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BACKGROUND: Methane() is considered as the secondmost potent greenhouse gas after carbon dioxide (). Methanogenesis is an enzyme-mediated multi-step process by methanogens. In the penultimate step, methylated Co-M is reduced by methyl Co-M reductase (MCR) to involving a nickel-containing cofactor F430. The activity of MCR enzyme is dependent on the F430 and therefore, the bioavailability of Ni to methanogens is expected to influence MCR activity and production in soil. In this study, different doses of EDTA(Ethylene Diamine Tetraacetic Acid) were applied in flooded soils to evaluate their suppression effect on methane production by chelating Ni of methanogenesis cofactor. METHODS AND RESULTS: EDTA was selected as chelating agents and added into wetland and rice paddy soil at the rates of 0, 25, 50, 75, and before 4-weeks incubation test. During the incubation, cumulative production patterns were characterized. At the end of the experiment, soil samples were removed from their jars to analyze total soil Ni and water-soluble Ni content and methanogen abundance. Methane production from 100 mmol application decreased by 55 and 78% in both soils compared to that from 0 mmol. With increasing application rate of EDTA in both soils, water-soluble Ni concentration significantly increased, but total soil Ni and methanogen activities showed negative relationship during incubation test. CONCLUSION: The decrease in methane production with EDTA application was caused by chelating Ni of coenzyme F430 and inhibiting methanogenesis by methyl coenzyme M reductase. Consequently, EDTA application decreased uptake of Ni into methanogen, subsequently inhibited methanogen activities and reduced methane production in flooded soils.
EDTA;Flooded soil;Methanogenesis;Ni;
 Cited by
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