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Toxicity Response of Biosensor Using Sulfur-Oxidizing Bacteria to Various Nitrogenous Compounds
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 Title & Authors
Toxicity Response of Biosensor Using Sulfur-Oxidizing Bacteria to Various Nitrogenous Compounds
Hwang, Ji-Hoon; Kang, Woo-Chang; Shin, Beom-Soo; Chae, Kyu-Jung; Oh, Sang-Eun;
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BACKGROUND: Run off from agricultural sites contaminates water bodies with nitrogen which is toxic and causes eutrophication when excessively accumulated. Hence, the interest in monitoring nitrogen toxicity in aquatic environment has been continuously increasing. METHODS AND RESULTS: To detect a real time toxicity of various nitrogen compounds, we applied biomonitoring method (biosensor) based on sulfur-oxidizing bacteria (SOB). The toxicity biomonitoring test was conducted in semi-continuous mode in a reactor filled with sulfur particles (2~4 mm diameter) under aerobic condition. Relative toxicity was simply determined by measuring the change in electrical conductivity (EC). Various nitrogenous compounds at different concentrations were evaluated as a potential toxic substance. Nitrite was found to be very toxic to SOB with a 90% inhibition even when the concentration as low as 3 mg/L. However, nitrate and ammonia have any inhibitory effect on SOB's activity. CONCLUSION: The biosensor based on SOB responded sensitively to nitrite even at substantially low concentrations. Therefore, it can be used as a reliable biological alarm system for rapid detection of contaminants due to its simplicity and sensitive nature.
Biosensor;Electrical Conductivity;Nitrogen;Sulfur Oxidizing Bacteria;Toxicity Monitoring System;
 Cited by
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