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Quantitative Analysis of Rotenone and Deguelin in Biopesticides Containing Derris Extract by Ultra performance Liquid Chromatography
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 Title & Authors
Quantitative Analysis of Rotenone and Deguelin in Biopesticides Containing Derris Extract by Ultra performance Liquid Chromatography
Lim, Sung-Jin; Kim, Jin-Hyo; Choi, Geun-Hyoung; Park, Byung-Jun;
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BACKGROUND: Three commercial biopesticides containing derris extract, which is permitted as a commercial biopesticide substances by the Environmentally-friendly Agriculture Promotion Act, have been marketed in Korea. But, the quantitative analytical method of active substances for crop protection in biopesticides containing derris extract has not known. METHODS AND RESULTS: Solid phase extraction (SPE) cartridge clean-up method for the quantitative analysis of rotenone and deguelin in biopesticides containing derris extract was developed and validated by ultra-performance liquid chromatography (UPLC). The clean-up method was established using hydrophilic lipophilic balance (HLB) SPE cartridges for the bioactive substances in biopesticides containing derris extract, and the eluate was analyzed to quantify the rotenone and deguelin by the UPLC. LOQ and recovery rates of rotenone and deguelin were 0.085 and 0.044 mg/L, 95.7 and 93.3%, respectively. The content of rotenone and deguelin in three biopesticides containing derris extract were analyzed by the developed method, the results showed 0.001-0.236 and
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