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Establishment of Analytical Method for Residues of Ethychlozate, a Plant Growth Regulator, in Brown Rice, Mandarin, Pepper, Potato, and Soybean Using HPLC/FLD
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 Title & Authors
Establishment of Analytical Method for Residues of Ethychlozate, a Plant Growth Regulator, in Brown Rice, Mandarin, Pepper, Potato, and Soybean Using HPLC/FLD
Kim, Jae-Young; Lee, Jin Hwan; Lee, Sang-Mok; Chae, Young-Sik; Rhee, Gyu-Seek; Chang, Moon-Ik;
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BACKGROUND: Ethychlozate (ECZ) is a plant growth regulator of synthetic auxin for agricultural commodities (ACs). Accurate and sensitive method to determine ECZ in diverse ACs on global official purpose is required to legal residue regulation. As the current official method is confined to the limited type of crops with poor validation, this study was conducted to improve and extend the ECZ method using high-performance liquid chromatography (HPLC) in all the registered crops with method verification. METHODS AND RESULTS: ECZ and its acidic metabolite (ECZA) were both extracted from acidified samples with acetone and briefly purified by dichloromethane partition. ECZ was hydrolyzed to form ECZA and the combined ECZA was finally purified by ion-associated partition including hexane-washing. The instrumental quantitation was performed using HPLC/ FLD under ion-suppression of ECZA with no interference by sample co-extractives. The average recoveries of intra- and inter-day experiment ranged from 82.0 to 105.2% and 81.7 to 102.8%, respectively. The repeatability and reproducibility for intra- and inter-day measurements expressed as a relative standard deviation was less than 8.7% and 7.4%, respectively. CONCLUSION: Established analytical method for ECZ residue in ACs was applicable to the nation-wide pesticide residues monitoring program with the acceptable level of sensitivity, repeatability and reproducibility.
Agricultural commodities (ACs);Analytical method;Ethychlozate (ECZ);HPLC/FLD;Plant growth regulator;
 Cited by
Association of Official Analytical Chemists International, (2010). Pesticide and industrial chemical residues, In official method of analysis, (18th ed.), pp. 17-26, Association of Official Analytical Chemists International, USA.

Aoki, Y., Kotani, A., Miyazawa, N., Uchida, K., Igarashi, Y., Hirayama, N., Hakamata, H., & Kusu, F. (2010). Determination of ethoxyquin by high-performance liquid chromatography with fluorescence detection and its application to the survey of residues in food products of animal origin. Journal of AOAC International, 93(1), 277-283.

Atta, S., Ikbal, M., Kumar, A., & Pradeep Singh, N. D. (2012). Application of photoremovable protecting group for controlled release of plant growth regulators by sunlight. Journal of Photochemistry and Photobiology B: Biology, 111, 39-49. crossref(new window)

BCPC. (2012). No. 340, Ethychlozate, In The Pesticide Manual: A World Compendium, (16th ed.), p. 441, British Crop Production Council, UK.

Berdikova Bohne, V. J., Hove, H., & Hamre, K. (2007). Simultaneous quantitative determination of the synthetic antioxidant ethoxyquin and its major metabolite in Atlantic salmon (Salmo salar, L), ethoxyquin dimer, by reversed-phase high-performance liquid chromatography with fluorescence detection. Journal of AOAC International, 90(2), 587-597.

Choi, J. H., Mamun, M. I. R., Abd El-Aty, A. M., Park, J. H., Shin, E. H., Park, J. Y., Cho, S. K., Shin, S. C., Lee, K.B., & Shim, J. H. (2011). Development of a single-step precipitation cleanup method for the determination of enrofloxacin, ciprofloxacin, and danofloxacin in porcine plasma. Food Chemistry, 127(4), 1878-1883. crossref(new window)

Do, J. A., Lee, M. Y., Cho, Y. J., Kang, I. H., Kwon, K. S., & Oh, J. H. (2013). Development and validation of an analytical method for pyrimisulfan determination in agricultural commodities by LC-MS/MS. Analytical Science and Technology, 26(2), 154-163. crossref(new window)

Giannakoula, A. E., Ilias, I. F., Maksimovic, J. J. D., Maksimovic, V. M., & Zivanovic, B. D. (2012). The effects of plant growth regulators on growth, yield, and phenolic profile of lentil plants. Journal of Food Composition and Analysis, 28(1), 46-53. crossref(new window)

Hem, L., Choi, J. H., Park, J. H., Mamun, M. I. R., Cho, S. K., Abd El-Aty, A. M., & Shim, J. H. (2011). Residual pattern of fenhexamid on pepper fruits grown under greenhouse conditions using HPLC and confirmation via tandem mass spectrometry. Food Chemistry, 126(4), 1533-1538. crossref(new window)

Kim, H. Y., Yoon, S. H., Park, H. J., Lee, J. H., Gwak, I. S., Moon, H. S., Song, M. H., Jang, Y. M., Lee, M. S., Park, J. S., & Lee, K. H. (2007). Monitoring of residual pesticides in commercial agricultural products in Korea. Korean Journal of Food Science and Technology, 39(3), 237-245.

Lee, H., Kim, E., Moon, J. K., Zhu, Y. Z., Do, J. A., Oh, J. H., Kwon, K., Lee, Y. D., & Kim, J. H. (2012). Establishment of analytical method for cyazofamid residue in apple, mandarin, korean cabbage, green pepper, potato and soybean. Journal of the Korean Society for Applied Biological Chemistry, 55(2), 241-247.

Lee, S. M., Kim, J. Y., Kim, T. H., Lee, H. J., Chang, M. I., Kim, H. J., Cho, Y. J., Choi, S. W., Kim, M. A., Kim, M. K., Rhee, G. S., & Lee, S. J. (2013). Establishment of analytical method for dichlorprop residues, a plant growth regulator in agricultural commodities using GC/ECD, Korean Journal of Environmental Agriculture, 32(3), 214-223. crossref(new window)

Min, Z. W., Hong, S. M., Yang, I. C., Kwon, H. Y., Kim, T. K., & Kim, D. H. (2012). Analysis of pesticide residues in brown rice using modified QuEChERS multiresidue method combined with electrospray ionization-liquid chromatography-tandem mass spectrometric detection. Journal of the Korean Society for Applied Biological Chemistry, 55(6), 769-775. crossref(new window)

Nissan Chemical Industry Ltd., Fujisawa Pharmaceutical Ltd. (1986). Summary of toxicity studies on ethychlozate, Journal of Pesticide Science, 11(1), 137-138.

Omeroglu, P. Y., Boyacioglu, D., Ambrus, A., Karaali, A., & Saner, S. (2012). An overview on steps of pesticide residue analysis and contribution of the individual steps to the measurement uncertainty. Food Analytical Methods, 5(6), 1469-1480. crossref(new window)

Park, J. Y., Choi, J. H., Abd El‐Aty, A. M., Kim, B. M., Park, J. H., Choi, W. J., & Shim, J. H. (2011). Development and validation of an analytical method for determination of endocrine disruptor, 2, 4‐D, in paddy field water. Biomedical Chromatography, 25(9), 1018-1024. crossref(new window)

Peterson, G. E. (1967). The discovery and development of 2, 4-D. Agricultural History, 41(3), 243-254.

Pihlstrom, T., Blomkvist, G., Friman, P., Pagard, U., & Osterdahl, B. G. (2007). Analysis of pesticide residues in fruit and vegetables with ethyl acetate extraction using gas and liquid chromatography with tandem mass spectrometric detection. Analytical and Bioanalytical Chemistry, 389(6), 1773-1789. crossref(new window)

Sharma, D., Nagpal, A., Pakade, Y. B., & Katnoria, J. K. (2010). Analytical methods for estimation of organophosphorus pesticide residues in fruits and vegetables: A review. Talanta, 82(4), 1077-1089. crossref(new window)

Spaepen, S., Vanderleyden, J., & Remans, R. (2007). Indole-3-acetic acid in microbial and microorganismplant signaling. FEMS Microbiology Reviews, 31(4), 425-448. crossref(new window)

Takatsuki, S., Nemoto, S., Sasaki, K., & Toyoda, M. (2002). Determination of ethychlozate and its degradation product in fruits by HPLC and LC/MS. Journal of the Food Hygienic Society of Japan, 43(1), 30-34. crossref(new window)

Taylor, M. J., Hunter, K., Hunter, K. B., Lindsay, D., & Le Bouhellec, S. (2002). Multi-residue method for rapid screening and confirmation of pesticides in crude extracts of fruits and vegetables using isocratic liquid chromatography with electrospray tandem mass spectrometry. Journal of Chromatography A, 982(2), 225-236. crossref(new window)

Thompson, M., Ellison, S. L. R., & Wood, R. (2002). Harmonized guidelines for single-laboratory validation of methods of analysis (IUPAC Technical Report). Pure and Applied Chemistry, 74(5), 835-855.

Tsipi, D., Triantafyllou, M., & Hiskia, A. (1999). Determination of organochlorine pesticide residues in honey, applying solid phase extraction with RP-C18 material. Analyst, 124(4), 473-475. crossref(new window)

Wang, K. S., Lu, C. Y., & Chang, S. H. (2011). Evaluation of acute toxicity and teratogenic effects of plant growth regulators by Daphniamagna embryo assay. Journal of Hazardous Materials, 190(1), 520-528. crossref(new window)

Wong, J. W., Zhang, K., Tech, K., Hayward, D. G., Krynitsky, A. J., Cassias, I., Schenck, F. J., Banerjee, K., Dasgupta, S., & Brown, D. (2010). Multiresidue Pesticide Analysis of Ginseng Powders Using Acetonitrile-or Acetone-Based Extraction, Solid-Phase Extraction Cleanup, and Gas Chromatography−Mass Spectrometry/Selective Ion Monitoring (GC-MS/SIM) or−Tandem Mass Spectrometry (GC-MS/MS). Journal of Agricultural and Food Chemistry, 58(10), 5884-5896. crossref(new window)