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Phylogeny of the Yeast Species Isolated from Wild Tiger Lily (Lilium lancifolium Thunb.)
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 Title & Authors
Phylogeny of the Yeast Species Isolated from Wild Tiger Lily (Lilium lancifolium Thunb.)
Kim, Jong-Shik; Kim, Dae-Shin;
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 Abstract
BACKGROUND: Yeast isolates associated with the leaves, stems, and flowers of the tiger lily needed to be identified using isolation methods that have previously been used effectively in yeast biotechnology. A culture-based approach was necessary for the isolation of many yeast strains associated with tiger lily. METHODS AND RESULTS: In this study, the homogenized leaves, stems, and flowers of tiger lily were spreaded onto GPY medium containing chloramphenicol, streptomycin, Triton X-100, and L-sorbose. A total of 82 yeast strains from the leaves, 94 and 97 yeast strains from the stems and flowers were isolated, respectively. Yeast isolates were identified by phylogenetic analysis based on internal transcribed spacer region sequencing. The yeast species isolated from the leaves comprised of 31 isolates of the genus Pseudozyma, 28 of Aureobasidium pullulans, and 11 of the genus Cryptococcus. Those isolated from the stems comprised of 40 of A. pullulans and 11 of Cryptococcus, and 95 of A. pullulans While, 1 isolate each of the genera Rhodotorula and Metschnikowia were isolated from the flowers. CONCLUSION: We identified site-specific yeast communities associated with tiger lily. These yeast isolates may have high potential for application in the field of biotechnology.
 Keywords
Aureobasidium pullulans;ITS gene;Tiger lily;Yeast;
 Language
Korean
 Cited by
1.
Yeasts Associated with Roots of the Endemic Plant Mankyua chejuense, Korean Journal of Environmental Agriculture, 2016, 35, 2, 137  crossref(new windwow)
2.
Yeasts in the Flowers of Wild Fleabane [Erigeron annus (L.) Pers.], Korean Journal of Environmental Agriculture, 2015, 34, 3, 238  crossref(new windwow)
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