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Comparison of Isoflavone Contents and Antioxidant Effect in Cheonggukjang with Black Soybean Cultivars by Bacillus subtilis CSY191
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 Title & Authors
Comparison of Isoflavone Contents and Antioxidant Effect in Cheonggukjang with Black Soybean Cultivars by Bacillus subtilis CSY191
Azizul Haque, Md.; Hwang, Chung Eun; Lee, Hee Yul; Ahn, Min Ju; Sin, Eui-Cheol; Nam, Sang Hae; Joo, Ok Soo; Kim, Hyun Joon; Lee, Shin-Woo; Kim, Yun-Geun; Ko, Keon Hee; Goo, Young-Min; Cho, Kye Man;
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BACKGROUND: Soybeans are the rich sources of isoflavones. To date, the changes of isoflavone contents in various black soybeans cheonggukjang during fermentation by Bacillus subtilis CSY191 has not been investigated.METHODS AND RESULTS: This study investigated the changes of total phenolic and isoflavone contents and antioxidant effects during cheonggukjang fermentation made with four black soybean (BS) cultivars including Cheongja, Cheongja#3, Geomjeong#5, and Ilpumgeomjeong with a potential probiotic Bacillus subtilis CSY191. The total phenolic contents, isoflavone-malonylglycoside and -aglycone contents, and antioxidant activity were increased in cheonggukjang at 48 h fermentation, while the content of isoflavone-glycosides was decreased during cheonggukjang fermentation. In particular, the Cheongja#3 soybean fermented at 37℃ for 48 h displayed the highest antioxidant activities, compared to those of the other BS cultivars tested. Also, the highest levels of total phenolic, daidzein, glycitein, and genistein were present at concentrations of 17.28 mg/g, 283.7 g/g, 39.9 g/g, and 13.2 g/g at the end of Cheongja#3 soybean fermentation.CONCLUSION: The results from this study suggested that the enhanced antioxidant activity of cheonggukjang of BS might be related to increased levels of total phenolic, isoflavon-aglycone, and malonyl-glycoside contents achieved during fermentation. Furthermore, fermented Cheongja#3 soybean showed the highest levels of enhanced antioxidant activities than the other BS cultivars.
Antioxidant;Bacillus subtilis CSY191;Black soybean;Cheonggukjang;Isoflavone;
 Cited by
Cho, K. M., Lee, J. H., Yun, H. D., Ahn, B. Y., Kim, H., & Seo, W. T. (2011). Changes of phytochemical constituents (isoflavones, flavanols, and phenolic acids) during cheonggukjang soybeans fermentation using potential probiotics Bacillus subtilis CS90. Journal of Food Composition and Analysis, 24(3), 402-410. crossref(new window)

Cho, K. M., Hong, S. Y., Math, R. K., Lee, J. H., Kambiranda, D. M., Kim, J. M., Islam, S. M. A., Yun, M. G., Cho, J. J., Lim, W. J., & Yun, H. D. (2009). Biotransformation of phenolics (isoflavones, flavanols and phenolic acids) during the fermentation of cheonggukjang by Bacillus pumilus HY1. Food Chemistry, 114(2), 413-419. crossref(new window)

Choi, J. S., Kim, H. Y., Seo, W. T., Lee, J. H., & Cho, K. M. (2012). Roasting enhances antioxidant effect of bitter melon (Momordica charantia L.) increasing in flavan-3-ol and phenolic acid contents. Food Science and Biotechnology, 21(1), 19-26 crossref(new window)

Coward, L., Smith, M., Kirk, M., & Barnes, S. (1998). Chemical modification of isoflavones in soyfoods during cooking and processing. American Journal of Clinical Nutrition, 68(6), 1486-1491.

Da Silva, L. H., Celeghini, R. M. S., & Chang, Y. K. (2011). Effect of the fermentation of whole soybean flour on the conversion of isolfavones from glycosides to aglycones. Food Chemistry, 128(3), 640-644. crossref(new window)

Hu, Y., Ge, C., Yuan, W., Zhu, R., Zhang, W., Du, L., & Xue, J. (2010). Characterization of fermented black soybean natto inoculated with Bacillus natto during fermentation. Journal of the Science of Food and Agriculture, 90(7), 1194-1202. crossref(new window)

Hwang, C. E., An, M. J., Lee, H. Y., Lee, B. W., Kim, H. T., Ko, J. M., Baek, I. Y., Seo, W. T., & Cho, K. M. (2014). Potential probiotic Lactobacillus plantarum P1201 to produce soy-yougurt with enhanced antioxidant activity. Korean Journal of Food Science and Technology, 46(5), 556-565. crossref(new window)

Hwang, C. E., Seo, W. T., & Cho, K. M. (2013). Enhanced antioxidant effect of black soybean by cheonggukjang with potential probiotic Bacillus subtilis CSY191. Korean Journal of Microbiology, 49(4), 391-397. crossref(new window)

Jang, C. H., Lim, J. K., Kim, J. H., Park, C. S., Kwon, D. Y., Kim, Y. S., Shin, D. H., & Kim, J. S. (2006). Change of isoflavone content during manufacturing of cheonggukjang, a traditional Korean fermented soy food. Food Science and Biotechnology, 15(4), 643-646.

Juan, M. Y., & Chou, C. C. (2010). Enhancement of antioxidant activity, total phenolic and flavonoid content of black soybeans by solid state fermentation with Bacillus subtilis BCRC 14715. Food Microbiology, 27(5), 586-591. crossref(new window)

Kim, H. G., Kim, G. W., Oh, H., Yoo, S. Y., Kim, Y. O., & Oh, M. S. (2011). Influence of roasting on the antioxidant activity of small black soybean (Glycine max L. Merrill). LWT-Food Science and Technology, 44(4), 992-998. crossref(new window)

Kim, J. S., Kang, O. J., & Gweon, O. C. (2013). Comparison of phenolic acids and flavonoids in black garlic at different thermal processing steps. Journal of Functional Foods, 5(1), 80-86. crossref(new window)

Kim, J. H., Hwan, C. E., Lee, C. K., Lee, J. H., Kim, G. M., Jeong, S. H., Shin, J. H., Kim, J. S., & Cho, K. M. (2014). Characteristics and antioxidant effect of garlic in the fermentation of cheonggukjang by Bacillus amyloliquefaciens MJ1-4. Journal of Microbiology and Biotechnology, 24(7), 959-968.

Kim, N. Y., Song, E. J., Kwon, D. Y., Kim, H. P., & Heo, M. Y. (2008). Antioxidant and antigenotoxic activities of Korean fermented soybean. Food and Chemical Toxicology, 46(3), 1184-1189. crossref(new window)

Kwak, C. S., Kim, S. A., & Lee, M. S. (2005). The correlation of antioxidative effects of 5 Korean common edible seaweeds and total polyphenol content. Journal of the Korean Society of Food Science and Nutrition, 34(8), 1143-1150. crossref(new window)

Kwak, C. S., Lee, M. S., & Park, S. C. (2007). Higher antioxidant of chungkookjang, a fermented soybean paste, may be due to increased aglycone and malonylgycoside isoflavone during fermentation. Nutrition Research, 27(110, 719-727. crossref(new window)

Lee, J. H., & Cho, K. M. (2012). Changes occurring in compositional components of black soybeans maintained at room temperature for different storage periods. Food Chemistry, 131(1), 161-169. crossref(new window)

Lee, J. H., & Choung, M. G. (2011). Determination of optimal acid hydrolysis time of soybean isoflavones using drying oven and microwave assisted methods. Food Chemistry, 129(2), 577-582. crossref(new window)

Lee, L. S., Choi, E. J., Kim, C. H., Kim, Y.B., Kum, J. S., & Park, J. D. (2014). Quality characteristics and antioxidant properties of black and yellow soybeans. Korean Journal of Food Science and Technology, 46(6), 757-761. crossref(new window)

Nam, Y. D., Yi, S. H., & Lim, S. I. (2012). Bacterial diversity of cheonggukjang, a traditional Korean fermented food, analyzed by barcoded pyrosequencing. Food Control, 28(1), 135-142. crossref(new window)

Prabhakaran, M. P., Perera, C. O., & Valiyaveettil, S. (2006). Effect of different coagulants on the isoflavones levels and physical properties of prepared firm tofu. Food Chemistry, 99(3), 492-499. crossref(new window)

Pratt, D. E. (1980). Natural antioxidants of soybeans and other oil seeds. In: Simic, M. G., Karel M, editors. Autoxidation in food and biological systems (pp. 283-293). Newyork: Plenum Press.

Shin, E. C., Lee, J. H., Hwang, C. E., Lee, B. W., Kim, H. T., Ko, J. M., Baek, I. Y., Shin, J. H., Nam, S. H., Seo, W. T., & Cho, K. M. (2014). Enhancement of total phenolic and isoflavone-aglycone contents and antioxidant activities during cheonggukjang fermentation of brown soybeans by the potential probiotic Bacillus subtilis CSY191. Food Science and Biotechnology, 23(2), 531-538. crossref(new window)

Shon, M. Y., Lee, J., Choi, S. Y., Nam, S. H., Seo, K. I., Lee, S. W., Sung, N. J., & Park, S. K. (2007). Antioxidant and free radical scavenging activity of methanol extract of chungkukjang. Journal of Food Composition and Analysis, 20(20, 113-118. crossref(new window)

Slavin, M., Cheng, Z., Luther, M., Kenworthy, W., & Yu, L. (2009). Antioxidant properties and phenolic, isoflavone, tocopherol and carotenoid composition of Maryland-grown soybean lines with altered fatty acid profiles. Food Chemistry, 114(1), 20-27. crossref(new window)

Velioglu, Y. S., Mazza, G., Gao, L., & Oomah, B. D. (1998). Antioxidant activity and total phenolics in selected fruits, vegetables and green products. Journal of Agricultural and Food Chemistry, 46(10), 4113-4117. crossref(new window)

Wang, L., Yin, L., Li, D., Zou, L., Saito, M., Tatsumi, E., & Li, L. (2007). Influecnes of processing and NaCl supplementation on isoflavone contents and composition during douche manufacturing. Food Chemistry, 101(3), 1247-1253. crossref(new window)

Yang, S. O., Chang, P. S., & Lee, J. H. (2006). Isoflavone distribution and -glycosidase activity in cheonggukjang, a traditional Korean whole soybean-fermented food. Food Science and Biotechnology, 15(1), 96-101.