JOURNAL BROWSE
Search
Advanced SearchSearch Tips
Yeasts Associated with Roots of the Endemic Plant Mankyua chejuense
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
Yeasts Associated with Roots of the Endemic Plant Mankyua chejuense
Kim, Jong-Shik; Kim, Dae-Shin; Jeon, Sang-Mi; Ko, Suk-Hyung;
  PDF(new window)
 Abstract
BACKGROUND: Identification of endophytic yeasts inhabiting the internal roots of the Mankyua chejuense tree requires techniques involving biotechnology. There is a need for a culture-based method to isolate and identify yeast strains associated with M. chejuense.METHODS AND RESULTS: We spread homogenized M. chejuense root samples onto glucose-peptone- yeast agar containing antibiotics, Triton X-100, and L-sorbose. A total of 152 yeast isolates were obtained and identified via phylogenetic analysis based on ITS gene sequencing. The results revealed that the root-associated yeast species included the genera Cyberlindnera (140 isolates), Candida (11 isolates), and Kluyveromyces (one isolate). Additionally, three yeast isolates showed high bioethanol production.CONCLUSION: We identified the specific yeast community associated with M. chejuense roots. These yeast isolates may have industrial applications as bioethanol producers. Our findings revealed that Cyberlindnera isolates included C. suaverolens and C. satumus, while Kluyveromyces isolates showed high bioethanol production.
 Keywords
Cyberlindnera;ITS gene;Mankyua chejuense;Roots;Yeast;
 Language
English
 Cited by
 References
1.
Botha, A. (2011). The importance and ecology of yeasts in soil. Soil Biology and Biochemistry, 43(1), 1-8. crossref(new window)

2.
Choi, S. C., Kim, M. U., and Kim, J. S. (2013). Selective isolation and phylogeny of the yeast species associated with Aloe vera and Aloe saponaria. Korean Journal of Environmental Agriculture, 32(3), 240-243. crossref(new window)

3.
Deak, T. (2009). Ecology and biodiversity of yeasts with potential value in biotechnology. Yeast biotechnology: diversity and applications (eds. Satyanarayana, T., and Kunze, G.), pp. 151-168. Springer Science + Business Media B. V., Dordrecht.

4.
Fonseca, A., and Inacio, J. (2006). Phylloplane yeasts. Biodiversity and ecophysiology of yeasts (eds. Rosa, C. A., and Peter, G.), pp. 263-301, Springer, Berlin.

5.
Hyun, H. J., Moon, M. O., Choi, H. S., and Kim, C. S. (2014). Growth pattern and phenology of Mankyua chejuense B. Y. Sun, M. H. Kim and C. H. Kim. Korean Journal of Plant Resources, 27(1), 95-101. crossref(new window)

6.
Hyeon, H. J., Kang, C. H., Song, K. M., and Kim, M. H. (2010). Flora and the conditions of Mankyua chejuense habitats. Korean Journal of Plant Resources, 23(4):350-359.

7.
Kim, C. H. (2004). Conservation status of the endemic fern Mankyua chejuense (Ophioglossaceae) on Cheju Island, Republic of Korea. Oryx, 38(2), 217-219. crossref(new window)

8.
Kim, J. S., and Kim, D. S. (2015a). Phylogeny of the yeast species isolated from wild tiger lily (Lilium lancifolium Thunb.). Korean Journal of Environmental Agriculture, 34(2), 149-154. crossref(new window)

9.
Kim, J. S., and Kim, D. S. (2015b). Yeasts in the flowers of wild Fleabane [Erigeron annus (L.) Pers]. Korean Journal of Environmental Agriculture, 34(3), 254-259.

10.
Kim, J. S., Lee, I. K., and Yun, B. S. (2015). A novel biosurfactant production by Aureobasidium pullulans L3-GPY from a Tiger lily wild flower, Lilium lancifolium Thunb. PLoS One, 10(4), e0122917 crossref(new window)

11.
Kim, J. S., Lee, I. K., Kim, D. W., and Yun, B. S. (2016). Aureosurfactin and 3-deoxyaureosurfactin, novel biosurfactants produced by Aureobasidium pullulans L3-GPY. The Journal of Antibiotics, doi: 10.1038/ja.2015.141. crossref(new window)

12.
Lee, K. M., Shin, J. H., Jeong, H. M., Kim, H. R., Kim, J. H., Shin, D. H., and You, Y. H. (2012). Characteristics of vegetation structure and habitat of Mankyua chejuense (Ophioglssaceae), endangered plant in Korea. Journal of Wetlands Research, 14(1), 35-45.

13.
Poomtien, J., Thaniyavarn, J., Pinphanichakarn, P., Jindamorakot, S., and Morikawa, M. (2013). Production and characterization of a biosurfactant from Cyberlindnera samutprakarnensis JP52T. Bioscience, Biotechnology, and Biochemistry, 77(12), 2362-2370. crossref(new window)

14.
Raspor, P., and Zupan, J. (2006). Yeast in extreme environments. Biodiversity and ecophysiology of yeasts (eds. Rosa, C. A., and Peter, G.), pp. 370-417. Springer, Berlin.

15.
Rosen, M. J., and Kunjappu, J. T. (2012). Surfactants and Interfacial Phenomena, pp. 471-501, John Wiley & Sons, Hoboken, NJ, USA

16.
Saitou, N., and Nei, M. (1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Molecular Biology and Evolution, 4(4), 406–425.

17.
Sun, B. Y., Kim, M. H., Kim, C. H., and Park, C. W. (2001). Mankyua (Ophioglossaceae): A new fern genus from Cheju Island, Korea. Taxon, 50(4), 1019-1024. crossref(new window)

18.
Tamang, J. P., and Fleet, G. H. (2009). Yeasts diversity in fermented foods and beverages. Yeast biotechnology: diversity and applications (eds. Satyanarayana, T., and Kunze, G.). pp. 169-198. Springer Science + Business Media B.V., Dordrecht.

19.
Tamura, K., Peterson, D., Peterson, N., Stecher, G, Nei, M., and Kumar, S. (2011). MEGA5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Molecular Biology and Evolution, 28(10), 2731-2739. crossref(new window)

20.
White, T. J., Bruns, T., Lee, S., and Taylor, J. (1990). Amplication and direct sequencing of fungal ribosomal RNA genes for phylogenetics. PCR protocols: a guide to methods and applications (eds. Innis, M. A., Gelfand, D. H., Sninsky, J. J., and White, T. J.), pp. 315-322, Academic press, San Diego.