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Identification of antigenic proteins of lymphocystis disease virus (LCDV) by MALDI-TOF mass spectrometry
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  • Journal title : Journal of fish pathology
  • Volume 28, Issue 3,  2015, pp.133-143
  • Publisher : The Korean Society of Fish Pathology
  • DOI : 10.7847/jfp.2015.28.3.133
 Title & Authors
Identification of antigenic proteins of lymphocystis disease virus (LCDV) by MALDI-TOF mass spectrometry
Chung, Chang-Kyun; Kim, Byung-Gwan; Jung, Myung-Hwa; Jung, Sung-Ju;
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 Abstract
The antigenic proteins of Lymphocystis disease virus (LCDV) from tumors of olive flounder, Paralichthys olivaceus, are described following characterization by mass spectrometry. In SDS-PAGE, predominant protein bands were observed at 114, 88, 70, 54, 52, 47, 42 and 24 kDa. Western blot analysis showed that antisera reacted strongly at molecular weights of 114, 67 and 54 kDa, and reacted weakly at molecular weights of 74, 70, 36, 24 and 22 kDa. In the identification of LCDV antigenic proteins by matrix-assisted laser desorption ionization (MALDI) TOF mass spectrometry, 10 of 14 excised bands consisted mostly of proteins with amino acid sequences that matched LCDV-C (lymphocystis disease virus isolate China) ORFs. Strong antigens with molecular weights of 114, 67 and 54 kDa were identified as LDVICp236 (chromosome segregation ATPase), LDVICp033 (membrane bound metallopeptidase) and LDVICp157 (hypothetical protein), respectively. Minor antigens with molecular weights of 70, 36, 24 and 22 kDa proteins were identified as LDVICp160 (acetyl-coA hydrolase), LDVICp213 (hypothetical protein), LDVICp039 (hypothetical protein) and LDVICp213 (hypothetical protein). However, the major capsid protein (LDVICp043) did not react with the polyclonal antibody.
 Keywords
Lymphocystis disease virus (LCDV);Antigenic proteins;MALDI-TOF mass spectrometry;Olive flounder;
 Language
English
 Cited by
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