Advanced SearchSearch Tips
Identification of antigenic proteins of lymphocystis disease virus (LCDV) by MALDI-TOF mass spectrometry
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
  • Journal title : Journal of fish pathology
  • Volume 28, Issue 3,  2015, pp.133-143
  • Publisher : The Korean Society of Fish Pathology
  • DOI : 10.7847/jfp.2015.28.3.133
 Title & Authors
Identification of antigenic proteins of lymphocystis disease virus (LCDV) by MALDI-TOF mass spectrometry
Chung, Chang-Kyun; Kim, Byung-Gwan; Jung, Myung-Hwa; Jung, Sung-Ju;
  PDF(new window)
The antigenic proteins of Lymphocystis disease virus (LCDV) from tumors of olive flounder, Paralichthys olivaceus, are described following characterization by mass spectrometry. In SDS-PAGE, predominant protein bands were observed at 114, 88, 70, 54, 52, 47, 42 and 24 kDa. Western blot analysis showed that antisera reacted strongly at molecular weights of 114, 67 and 54 kDa, and reacted weakly at molecular weights of 74, 70, 36, 24 and 22 kDa. In the identification of LCDV antigenic proteins by matrix-assisted laser desorption ionization (MALDI) TOF mass spectrometry, 10 of 14 excised bands consisted mostly of proteins with amino acid sequences that matched LCDV-C (lymphocystis disease virus isolate China) ORFs. Strong antigens with molecular weights of 114, 67 and 54 kDa were identified as LDVICp236 (chromosome segregation ATPase), LDVICp033 (membrane bound metallopeptidase) and LDVICp157 (hypothetical protein), respectively. Minor antigens with molecular weights of 70, 36, 24 and 22 kDa proteins were identified as LDVICp160 (acetyl-coA hydrolase), LDVICp213 (hypothetical protein), LDVICp039 (hypothetical protein) and LDVICp213 (hypothetical protein). However, the major capsid protein (LDVICp043) did not react with the polyclonal antibody.
Lymphocystis disease virus (LCDV);Antigenic proteins;MALDI-TOF mass spectrometry;Olive flounder;
 Cited by
Cheng, S., Zhan, W., Xing, J. and Sheng, X.: Development and characterization of monoclonal antibody to the lymphocytis disease virus of Japanese flounder Paralichthys olivaceus isolate from China. J. Virol. Methods, 135: 173-180, 2006. crossref(new window)

Chinchar, V.G., Metzger, D.W., Granoff, A. and Goorha, R.: Localization of frog virus 3 proteins using monoclonal antibodies. Virology, 137: 211-216, 1984. crossref(new window)

Darai, G., Anders, K., Koch, H.G., Delius, H., Gelderblom, H., Samalecos, C. and Flugel, R.M.: Analysis of the genome of fish lymphocystis disease virus isolated directly from epidermal tumors of pleuronectes. Virology, 126: 466-479, 1983. crossref(new window)

Davison, S., Carne, A., McMillan, N.A.J. and Kalmakoff, J.: A comparison of the structural polypeptides of three iridescent viruses (type 6, 9 and 16) and the mapping of the DNA region coding for their major capsid protein polypeptides. Arch. Virol., 123: 229-237, 1992. crossref(new window)

Fluguel, R.M., Darai, G. and Gelderblom, H.: Viral protein and adenosine triphosphate phosphohydrolase activity of fish lympocysitis disease virus. Virology, 122: 48-55, 1982. crossref(new window)

Garcia-Rosado, E., Castro, D., Cano, I., Alonso, M.C., Perez-Prieto, S.I. and Borrego, J.J.: Protein and glycoprotein content of lymphocystis disease virus (LCDV). Int. Microbiol., 7: 121-126, 2004.

Hossain, M., Song, J.Y., Kitamura, S.I, Jung, S.J. and Oh, M.J.: Phylogenietic analysis of lymphosystis diease virus from tropical ornamental fish species based on a major capcid protein gene. J. Fish Dis., 31: 473-479, 2008. crossref(new window)

Iwamoto, R., Hasegawa, O., Lapatra, S. and Yoshimizu, M.: Isolation and characterization of the Japanease flounder (Paralichthys olivaceaus) lymphocystis disease virus. J. Aquat. Anim. Health., 14: 114-123, 2002. crossref(new window)

Kim, W.S., Oh, M.J., Nishizawa, T., Park, J.W., Kurath, G. and Yoshimizu, M.: Non-specific absorption of fish immunoglobulin M (IgM) to blocking reagents on ELISA plate wells. Dis. Aquat. Organ., 78: 55-59, 2007. crossref(new window)

Kim, T.J., Jang, E.J. and Lee, J.I.: Vaccination of rock bream, Oplegnathus fasciatus (Temminck & Shlegel), using a recombinant major capsid protein of fish iridovirus. J. Fish Dis., 31: 547-551, 2008. crossref(new window)

Kitamura, S.I., Jung, S.J., Kim, W.S., Nishizawa, T., Yoshimizu, M. and Oh, M.J.: A new genotype of lymphocystis disease virus, LCDV-RF, from lymphocystis disease rockfish. Arch. Virol., 151: 607-615, 2006a. crossref(new window)

Kitamura, S.I., Jung, S.J. and Oh, M.J.: Differentiation of lymphocystis disease virus genotype by multiplex PCR. J. Microbiol., 44: 248-253, 2006b.

Kitamura, S.I., Ko, J.Y., Lee, W.R., Kim, S.R., Song, J.Y., Kim, D.K., Jung, S.J. and Oh, M.J.: Seasonal prevalence of lymphocystis disease virus and aquabirnavirus in Japanese flounder, Paralichthys olivaceus and blue mussel, Mytilus galloprovincialis. Aquagulture, 266: 26-31, 2007.

Laemmli U.K.: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227: 680-685, 1970. crossref(new window)

Monini, M. and Ruggeri, F.M.: Antigenic peptides of the epizootic hematopoietic necrosis virus, Virology, 297: 8-18, 2002. crossref(new window)

Nakajima, K., Maeno, Y., Yokoyama, K., Kaji, C. and Manabe, S.: Antigenic analysis of red sea bream iridovirus and comparison with other fish iridoviruses. Fish Pathol., 33: 73-78, 1998. crossref(new window)

Pandey, A. and Mann, M.: Proteomics to study genes and genomes. Nature, 405: 837-846, 2002.

Peters, N. and Schmidt, W.: Formation and disintergration of virions in lymphocystis cells of plaice Pleuronectes platessa. Dis. Aquat. Organ., 21: 109-113, 1995. crossref(new window)

Qin, Q.W., Shi, C., Gin, K.Y.M., Gin, Y.H. and Lam, T.J.: Antigenic characterization of a marine fish iridovirus from grouper, Epinephelus spp. J. Virol. Methods, 106: 889-896, 2002.

Schnitzler, P., Delius, H., Scholz, J., Tourau, M., Orth, E. and Darai, G.: Identification and nucleotide sequence analysis of repetitive DNA element in the genome of fish lymphocystis virus. Virology, 161: 570-578, 1987. crossref(new window)

Schnitzler, P. and Darai, G.: Identification of the gene encoding the major capsid protein of fish lymphocystis disease virus. J. Gen. Virol., 74: 2143-2150, 1993. crossref(new window)

Steiner, K.A., Whittington, R.J., Petersen, R.K., Hornitzky, C. and Garnett, H.: Purification of epizootic haematopoietic necrosis virus and its detection using ELISA. J. Virol. Methods, 33: 199-209, 1991.

Tidona, C.A. and Darai, G.: The complete DNA sequence of lymphocystis disease virus. Virology, 238: 353-362, 1997. crossref(new window)

Walker, D.P. and Hill, B.J.: Studies on the culture, assay of infectivity and some in vitro properties of lymphocystis virus. J. Gen. Virol., 51: 385-395, 1980. crossref(new window)

Walker, R.: Fine structure of lymphocystis virus of fish. Virology, 18: 503-505, 1962. crossref(new window)

Wolf, K.: Lymphocystis disease. Fish Viruses and Fish Viral Disease. Cornell University Press. Ithaca, NY, 1988.

Zhang, Q.Y., Xiao, F., Xie, J., Li, Z.Q. and Gui, J.F.: Complete genome sequence of lymphocystis disease virus isolate from china. J. Virol., 78: 6982-6994, 2004. crossref(new window)

Zheng, F.R., Sun, X.Q., Liu, H.Z. and Zhang, J.X.: Study on the distribution and expression of a DNA vaccine against lymphocystis disease virus in Japanese flounder (Paralichthys olivaceus). Aquaculture, 261: 1128-1134, 2006. crossref(new window)

Zwillenberg, L.O. and Wolf, K.: Utrastructure of lymphocystis virus. J. Virol., 2: 393-399, 1968.