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The Effect of pH and Temperature on Lysozyme Separation in Ion-exchange Chromatography
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  • Journal title : Korean Chemical Engineering Research
  • Volume 52, Issue 1,  2014, pp.98-105
  • Publisher : The Korean Institute of Chemical Engineers
  • DOI : 10.9713/kcer.2014.52.1.98
 Title & Authors
The Effect of pH and Temperature on Lysozyme Separation in Ion-exchange Chromatography
Ko, Kwan-Young; Kim, In-Ho;
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Lysozyme amounts to 0.3% in egg white and functions as an agent of cell lysis and activator of tissue reconstruction. Ion exchange chromatography is the most useful method of separation among affinity chromatography, ion exchange chromatography, and ultra-filtration. The aim of present study is to find the optimum pH and temperature for the separation of lysozyme in egg white within cation exchange gel filled glass column. And we compared results of experiments with those of simulations. Phosphate buffer was used, and pH and temperature were varied as 5~7 and respectively. RP-HPLC was the tool for the retention time identification and quantitative analysis of lysozyme. OriginPro 8 measured the peak area of lysozyme chromatogram and quantified the eluted lysozyme. Largest amount of lysozyme was separated under the conditions of pH 5 and T .
Lysozyme;Ion Exchange Chromatography;Aspen Simulation;
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