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Droplet-based Microfluidic Device for High-throughput Screening
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  • Journal title : Korean Chemical Engineering Research
  • Volume 52, Issue 2,  2014, pp.141-153
  • Publisher : The Korean Institute of Chemical Engineers
  • DOI : 10.9713/kcer.2014.52.2.141
 Title & Authors
Droplet-based Microfluidic Device for High-throughput Screening
Jeong, Heon-Ho; Noh, Young-Moo; Jang, Sung-Chan; Lee, Chang-Soo;
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 Abstract
Droplet based microfluidic systems have been developed for the application of biological and chemical research field. A picoliter droplet in microfluidic device provides a compartmentalized and well-defined reactor in miniaturized system. The microfluidic system with small droplets can reduce reagent cost and enhance efficiency through automated high-throughput screening system. In this review, we summarize the functionality of droplet based microfluidic system including droplet generation, precise droplet control, and various applications. In addition, this article reviews current applications in chemistry and biology, and discuss advantages of droplet based microfluidics compared with conventional manner.
 Keywords
Microfluidic Device;Droplet;High-throughput Screening;Micro-reactor;Emulsion;
 Language
Korean
 Cited by
1.
은 나노입자 전극과 패러데이 모트를 이용한 미세유체 피코리터 주입기의 전압효율 상승,노영무;진시형;정성근;김남영;노창현;이창수;

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 References
1.
Huebner, A., S. Sharma, M. Srisa-Art, F. Hollfelder, J. B. Edel, and A. J. Demello, "Microdroplets: A Sea of Applications?," Lab Chip, 8(8), 1244-1254(2008). crossref(new window)

2.
Theberge, A. B., Courtois, F., Schaerli, Y., Fischlechner, M., Abell, C., Hollfelder, F. and Huck, W. T. S., "Microdroplets in Microfluidics: An Evolving Platform for Discoveries in Chemistry and Biology," Angew. Chem. Int. Edit., 49(34), 5846-5868(2010). crossref(new window)

3.
Kovarik, M. L., Gach, P. C., Ornoff, D. M. Wang, Y. L., Balowski, J., Farrag, L. and Allbritton, N. L., "Micro Total Analysis Systems for Cell Biology and Biochemical Assays," Anal. Chem., 84(2), 516-540(2012). crossref(new window)

4.
Whitesides, G. M., "The Origins and the Future of Microfluidics," Nature, 442(7101), 368-373(2006). crossref(new window)

5.
Song, H. M. and Lee, C. S., "Simple Fabrication of Functionalized Surface with Polyethylene Glycol Microstructure and Glycidyl Methacrylate Moiety for the Selective Immobilization of Proteins and Cells," Korean J. Chem. Eng., 25(6), 1467-1472(2008). crossref(new window)

6.
Lee, J. H., Kim, H. E., Im, J. H., Bae, Y. M., Choi, J. S., Huh, K. M. and Lee, C. S., "Preparation of Orthogonally Functionalized Surface Using Micromolding in Capillaries Technique for the Control of Cellular Adhesion," Colloids Surf. B: Biointerfaces, 64(1), 126-134(2008). crossref(new window)

7.
Chiu, D. T., Lorenz, R. M. and Jeffries, G. D. M., "Droplets for Ultrasmall-Volume Analysis," Anal. Chem., 81(13), 5111-5118(2009). crossref(new window)

8.
Choi, C. H., Prasad, N., Lee, N. R. and Lee, C. S., "Investigation of Microchannel Wettability on the Formation of Droplets and Efficient Mixing in Microfluidic Devices," Biochip J., 2(1), 27-32 (2008).

9.
Teh, S. Y., Lin, R., Hung, L. H. and Lee, A. P., "Droplet Microfluidics," Lab Chip, 8(2), 198-220(2008). crossref(new window)

10.
Jeong, H. H., Lee, S. H., Kim, J. M., Kim, H. E., Kim, Y. G., Yoo, J. Y., Chang, W. S. and Lee, C. S., "Microfluidic Monitoring of Pseudomonas Aeruginosa Chemotaxis Under the Continuous Chemical Gradient," Biosens. Bioelectron., 26(2), 351-6(2010). crossref(new window)

11.
Kim, K. P., Kim, Y. G., Choi, C. H., Kim, H. E., Lee, S. H., Chang, W. S. and Lee, C. S., "In situ Monitoring of Antibiotic Susceptibility of Bacterial Biofilms in a Microfluidic Device," Lab Chip, 10(23), 3296-9(2010). crossref(new window)

12.
Cira, N. J., Ho, J. Y., Dueck, M. E. and Weibel, D. B., "A Selfloading Microfluidic Device for Determining the Minimum Inhibitory Concentration of Antibiotics," Lab Chip, 12(6), 1052-1059(2012). crossref(new window)

13.
Kaigala, G. V., Hoang, V. N., Stickel, A., Lauzon, J., Manage, D., Pilarski, L. M. and Backhouse, C. J., "An Inexpensive and Portable Microchip-Based Platform for Integrated RT-PCR and Capillary Electrophoresis," Analyst, 133(3), 331-338(2008). crossref(new window)

14.
Jeong, H. H., Lee, S. H. and Lee, C. S., "Pump-less Static Microfluidic Device for Analysis of Chemotaxis of Pseudomonas Aeruginosa Using Wetting and Capillary Action," Biosens. Bioelectron., 47, 278-84(2013). crossref(new window)

15.
Jung, J. H., Choi, C. H., Hwang, T. S. and Lee, C. S., "Efficient In situ Production of Monodisperse Polyurethane Microbeads in Microfluidic Device using Increase of Residence Time of Droplets," Biochip J., 3(1), 44-49(2009).

16.
Garstecki, P., Fuerstman, M. J., Stone, H. A. and Whitesides, G. M., "Formation of Droplets and Bubbles in a Microfluidic Tjunction - scaling and Mechanism of Break-up," Lab Chip, 6(3), 437-446(2006). crossref(new window)

17.
Song, H. and Ismagilov, R. F., "Millisecond Kinetics on a Microfluidic Chip Using Nanoliters of Reagents," J. Am. Chem. Soc., 125(47), 14613-14619(2003). crossref(new window)

18.
Clausell-Tormos, J., Lieber, D., Baret, J. C., El-Harrak, A., Miller, O. J., Frenz, L., Blouwolff, J., Humphry, K. J., Koster, S., Duan, H., Holtze, C., Weitz, D. A., Griffiths, A. D. and Merten, C. A., "Droplet-based Microfluidic Platforms for the Encapsulation and Screening of Mammalian Cells and Multicellular Organisms (vol 15, pg 427, 2008)," Chem. Biol., 15(8), 875-875 (2008). crossref(new window)

19.
Jung, S. Y., Liu, Y. and Collier, C. P., "Fast Mixing and Reaction Initiation Control of Single-enzyme Kinetics in Confined Volumes," Langmuir, 24(9), 4439-4442(2008). crossref(new window)

20.
Courtois, F., Olguin, L. F., Whyte, G., Theberge, A. B., Huck, W. T. S., Hollfelder, F. and Abell, C., "Controlling the Retention of Small Molecules in Emulsion Microdroplets for Use in Cell-Based Assays," Anal. Chem., 81(8), 3008-3016(2009). crossref(new window)

21.
Liau, A., Karnik, R., Majumdar, A. and Cate, J. H. D., "Mixing Crowded Biological Solutions in Milliseconds," Anal. Chem., 77(23), 7618-7625(2005). crossref(new window)

22.
Tan, Y. C., Ho, Y. L. and Lee, A. P., "Droplet Coalescence by Geometrically Mediated Flow in Microfluidic Channels," Microfluid. Nanofluid., 3(4), 495-499(2007). crossref(new window)

23.
Huebner, A., Bratton, D., Whyte, G., Yang, M., deMello, A. J., Abell, C. and Hollfelder, F., "Static Microdroplet Arrays: a Microfluidic Device for Droplet Trapping, Incubation and Release for Enzymatic and Cell-based Assays," Lab Chip, 9(5), 692-698(2009). crossref(new window)

24.
Simon, M. G., Lin, R., Fisher, J. S. and Lee, A. P., "A Laplace Pressure Based Microfluidic Trap for Passive Droplet Trapping and Controlled Release," Biomicrofluidics, 6(1), (2012).

25.
Shim, J. U., Cristobal, G., Link, D. R., Thorsen, T., Jia, Y. W., Piattelli, K. and Fraden, S., "Control and Measurement of the Phase Behavior of Aqueous Solutions Using Microfluidics," J. Am. Chem. Soc., 129(28), 8825-8835(2007). crossref(new window)

26.
Schafle, C., Bechinger, C., Rinn, B., David, C. and Leiderer, P., "Cooperative Evaporation in Ordered Arrays of Volatile Droplets,"Phys. Rev. Lett., 83(25), 5302-5305(1999). crossref(new window)

27.
Yun, J. X., Tu, C. M., Lin, D. Q., Xu, L. H., Guo, Y. T., Shen, S. C., Zhang, S. H., Yao, K. J., Guan, Y. X. and Yao, S. J., "Microchannel Liquid-flow Focusing and Cryo-polymerization Preparation of Supermacroporous Cryogel Beads for Bioseparation," J. Chromatogr. A, 1247, 81-88(2012). crossref(new window)

28.
Lee, W. S., Jambovane, S., Kim, D. and Hong, J. W., "Predictive Model on Micro Droplet Generation Through Mechanical Cutting," Microfluid. Nanofluid., 7(3), 431-438(2009). crossref(new window)

29.
Fair, R. B., "Digital Microfluidics: is a True Lab-on-a-chip Possible?," Microfluid. Nanofluid., 3(3), 245-281(2007). crossref(new window)

30.
Du, W. B., Sun, M., Gu, S. Q., Zhu, Y. and Fang, Q., "Automated Microfluidic Screening Assay Platform Based on Drop Lab," Anal. Chem., 82(23), 9941-9947(2010). crossref(new window)

31.
Zeng, S. J., Li, B. W., Su, X. O., Qin, J. H. and Lin, B. C., "Microvalve-actuated Precise Control of Individual Droplets in Microfluidic Devices," Lab Chip, 9(10), 1340-1343(2009). crossref(new window)

32.
Hong, J., Choi, M., Edel, J. B. and deMello, A. J., "Passive Selfsynchronized Two-droplet Generation," Lab Chip, 10(20), 2702-2709(2010). crossref(new window)

33.
Ahn, B., Lee, K., Lee, H., Panchapakesan, R. and Oh, K. W., "Parallel Synchronization of Two Trains of Droplets Using a Railroad-like Channel Network," Lab Chip, 11(23), 3956-3962(2011). crossref(new window)

34.
Christopher, G. F., Bergstein, J., End, N. B., Poon, M., Nguyen, C. and Anna, S. L., "Coalescence and Splitting of Confined Droplets at Microfluidic Junctions," Lab Chip, 9(8), 1102-1109(2009). crossref(new window)

35.
Choi, J. H., Lee, S. K., Lim, J. M., Yang, S. M. and Yi, G. R., "Designed Pneumatic Valve Actuators for Controlled Droplet Breakup and Generation," Lab Chip, 10(4), 456-461(2010). crossref(new window)

36.
Link, D. R., Anna, S. L., Weitz, D. A. and Stone, H. A., "Geometrically Mediated Breakup of Drops in Microfluidic Devices," Phys. Rev. Lett., 92(5), (2004).

37.
Churski, K., Korczyk, P. and Garstecki, P., "High-throughput Automated Droplet Microfluidic System for Screening of Reaction Conditions," Lab Chip, 10(7), 816-818(2010). crossref(new window)

38.
Hong, J., Choi, M., deMello, A. J. and Edel, J. B., "Interfacial Tension-Mediated Droplet Fusion in Rectangular Microchannels," Biochip J., 3(3), 203-207(2009).

39.
Niu, X., Gulati, S., Edel, J. B. and deMello, A. J., "Pillar-induced Droplet Merging in Microfluidic Circuits," Lab Chip, 8(11), 1837-1841(2008). crossref(new window)

40.
Mazutis, L. and Griffiths, A. D., "Selective Droplet Coalescence Using Microfluidic Systems," Lab Chip, 12(10), 1800-1806(2012). crossref(new window)

41.
Tan, W. H. and Takeuchi, S., "A Trap-and-release Integrated Microfluidic System for Dynamic Microarray Applications," Proc. Natl. Acad. Sci. USA, 104(4), 1146-1151(2007). crossref(new window)

42.
Boukellal, H., Selimovic, S., Jia, Y. W., Cristobal, G. and Fraden, S., "Simple, Robust Storage of Drops and Fluids in a Microfluidic Device," Lab Chip, 9(2), 331-338(2009). crossref(new window)

43.
Bai, Y. P., He, X. M., Liu, D. S., Patil, S. N., Bratton, D., Huebner, A., Hollfelder, F., Abell, C. and Huck, W. T. S., "A Double Droplet Trap System for Studying Mass Transport Across a Droplet-droplet Interface," Lab Chip, 10(10), 1281-1285(2010). crossref(new window)

44.
Leung, K., Zahn, H., Leaver, T., Konwar, K. M., Hanson, N. W., Page, A. P., Lo, C. C., Chain, P. S., Hallam, S. J. and Hansen, C. L., "A Programmable Droplet-based Microfluidic Device Applied to Multiparameter Analysis of Single Microbes and Microbial Communities," Proc. Natl. Acad. Sci. USA, 109(20), 7665-7670(2012). crossref(new window)

45.
Cohen, I., Li, H., Hougland, J. L., Mrksich, M. and Nagel, S. R., "Using Selective Withdrawal to Coat Microparticles," Science, 292(5515), 265-267(2001). crossref(new window)

46.
Chabert, M. and Viovy, J. L., "Microfluidic High-throughput Encapsulation and Hydrodynamic Self-sorting of Single Cells," Proc. Natl. Acad. Sci. USA, 105(9), 3191-3196(2008). crossref(new window)

47.
Yiantsios, S. G. and Davis, R. H., "On the Buoyancy-Driven Motion of a Drop Towards a Rigid Surface or a Deformable Interface," J. Fluid. Mech., 217, 547-573(1990). crossref(new window)

48.
Cubaud, T. and Mason, T. G., "Folding of Viscous Threads in Diverging Microchannels," Phys. Rev. Lett., 96(11), (2006).

49.
Agresti, J. J., Antipov, E., Abate, A. R., Ahn, K., Rowat, A. C., Baret, J. C., Marquez, M., Klibanov, A. M., Griffiths, A. D. and Weitz, D. A., "Ultrahigh-throughput Screening in Drop-based Microfluidics for Directed Evolution (vol 170, pg 4004, 2010)," Proc. Natl. Acad. Sci. USA, 107(14), 6550-6550(2010). crossref(new window)

50.
Wang, W., Yang, C., Liu, Y. S. and Li, C. M., "On-demand Droplet Release for Droplet-based Microfluidic System," Lab Chip, 10(5), 559-562(2010). crossref(new window)

51.
Hatakeyama, T., Chen, D. L. and Ismagilov, R. F., "Microgramscale Testing of Reaction Conditions in Solution Using Nanoliter Plugs in Microfluidics with Detection by MALDI-MS," J. Am. Chem. Soc., 128(8), 2518-2519(2006). crossref(new window)

52.
Theberge, A. B., Whyte, G., Frenzel, M., Fidalgo, L. M., Wootton, R. C. R. and Huck, W. T. S., "Suzuki-Miyaura Coupling Reactions in Aqueous Microdroplets with Catalytically Active Fluorous Interfaces," Chem Commun., 41, 6225-6227(2009).

53.
Miyaura, N. and Suzuki, A., "Palladium-Catalyzed Cross-Coupling Reactions of Organoboron Compounds," Chem. Rev., 95(7), 2457-2483(1995). crossref(new window)

54.
Li, S. H., Lin, Y. J., Cao, J. G. and Zhang, S. B., "Guanidine/Pd(OAc)(2)-catalyzed Room Temperature Suzuki Cross-coupling Reaction in Aqueous Media Under Aerobic Conditions," J. Org. Chem., 72(11), 4067-4072(2007). crossref(new window)

55.
Prasad, N., Perumal, J., Choi, C. H., Lee, C. S. and Kim, D. P., "Generation of Monodisperse Inorganic-Organic Janus Microspheres in a Microfluidic Device," Adv. Funct. Mater., 19(10), 1656-1662(2009). crossref(new window)

56.
Chan, E. M., Alivisatos, A. P. and Mathies, R. A., "High-temperature Microfluidic Synthesis of CdSe Nanocrystals in Nanoliter Droplets," J. Am. Chem. Soc., 127(40), 13854-13861(2005). crossref(new window)

57.
Jung, J. H., Park, T. J., Lee, S. Y. and Seo, T. S., "Homogeneous Biogenic Paramagnetic Nanoparticle Synthesis Based on a Microfluidic Droplet Generator," Angew. Chem. Int. Edit., 51(23), 5634-5637(2012). crossref(new window)

58.
Abou Hassan, A., Sandre, O., Cabuil, V. and Tabeling, P., "Synthesis of Iron Oxide Nanoparticles in a Microfluidic Device: Preliminary Results in a Coaxial Flow Millichannel," Chem Commun., 15, 1783-1785(2008).

59.
Zhao, C. X., He, L. Z., Qiao, S. Z. and Middelberg, A. P. J., "Nanoparticle Synthesis in Microreactors," Chem Eng Sci., 66(7), 1463-1479(2011). crossref(new window)

60.
Lu, A. H., Salabas, E. L. and Schuth, F., "Magnetic Nanoparticles: Synthesis, Protection, Functionalization, and Application," Angew. Chem. Int. Edit., 46(8), 1222-1244(2007). crossref(new window)

61.
Jeong, H. H., Kim, Y. G., Jang, S. C., Yi, H. M. and Lee, C. S., "Profiling Surface Glycans on Live Cells and Tissues Using Quantum Dot-lectin Nanoconjugates," Lab Chip, 12(18), 3290-3295(2012). crossref(new window)

62.
Kim, J., Chung, Y. M., Kang, S. M., Choi, C. H., Kim, B. Y., Kwon, Y. T., Kim, T. J., Oh, S. H. and Lee, C. S., "Palladium Nanocatalysts Immobilized on Functionalized Resin for the Direct Synthesis of Hydrogen Peroxide from Hydrogen and Oxygen," ACS Catal., 2(6), 1042-1048(2012). crossref(new window)

63.
Bharde, A., Rautaray, D., Bansal, V., Ahmad, A., Sarkar, I., Yusuf, S. M., Sanyal, M. and Sastry, M., "Extracellular Biosynthesis of Magnetite Using Fungi," Small, 2(1), 135-141(2006). crossref(new window)

64.
Duraiswamy, S. and Khan, S. A., "Plasmonic Nanoshell Synthesis in Microfluidic Composite Foams," Nano Lett, 10(9), 3757-3763(2010). crossref(new window)

65.
Rubin, A. E., Tummala, S., Both, D. A., Wang, C. C. and Delaney, E. J., "Emerging Technologies Supporting Chemical Process R&D and Their Increasing Impact on Productivity in the Pharmaceutical Industry," Chem. Rev., 106(7), 2794-2810(2006). crossref(new window)

66.
An, H. Y. and Cook, P. D., "Methodologies for Generating Solution-phase Combinatorial Libraries," Chem. Rev., 100(9), 3311-3340(2000). crossref(new window)

67.
Theberge, A. B., Mayot, E., El Harrak, A., Kleinschmidt, F., Huck, W. T. S. and Griffiths, A. D., "Microfluidic Platform for Combinatorial Synthesis in Picolitre Droplets," Lab Chip, 12(7), 1320-1326(2012). crossref(new window)

68.
Zhang, C. S., Xing, D. and Li, Y. Y., "Micropumps, Microvalves, and Micromixers Within PCR Microfluidic Chips: Advances and Trends," Biotechnol Adv, 25(5), 483-514(2007). crossref(new window)

69.
Hindson, B. J., Ness, K. D., Masquelier, D. A., Belgrader, P., Heredia, N. J., Makarewicz, A. J., Bright, I. J., Lucero, M. Y., Hiddessen, A. L., Legler, T. C., Kitano, T. K., Hodel, M. R., Petersen, J. F., Wyatt, P. W., Steenblock, E. R., Shah, P. H., Bousse, L. J., Troup, C. B., Mellen, J. C., Wittmann, D. K., Erndt, N. G., Cauley, T. H., Koehler, R. T., So, A. P., Dube, S., Rose, K. A., Montesclaros, L., Wang, S. L., Stumbo, D. P., Hodges, S. P., Romine, S., Milanovich, F. P., White, H. E., Regan, J. F., Karlin-Neumann, G. A., Hindson, C. M., Saxonov, S. and Colston, B. W., "High-Throughput Droplet Digital PCR System for Absolute Quantitation of DNA Copy Number," Anal. Chem., 83(22), 8604-8610(2011). crossref(new window)

70.
Kiss, M. M., Ortoleva-Donnelly, L., Beer, N. R., Warner, J., Bailey, C. G., Colston, B. W., Rothberg, J. M., Link, D. R. and Leamon, J. H., "High-Throughput Quantitative Polymerase Chain Reaction in Picoliter Droplets," Anal. Chem., 80(23), 8975-8981(2008). crossref(new window)

71.
Schaerli, Y., Wootton, R. C., Robinson, T., Stein, V., Dunsby, C., Neil, M. A. A., French, P. M. W., deMello, A. J., Abell, C. and Hollfelder, F., "Continuous-Flow Polymerase Chain Reaction of Single-Copy DNA in Microfluidic Microdroplets," Anal. Chem., 81(1), 302-306(2009). crossref(new window)

72.
Hatch, A. C., Fisher, J. S., Tovar, A. R., Hsieh, A. T., Lin, R., Pentoney, S. L., Yang, D. L. and Lee, A. P., "1-Million Droplet Array with Wide-field Fluorescence Imaging for Digital PCR," Lab Chip., 11(22), 3838-3845(2011). crossref(new window)

73.
Fallah-Araghi, A., Baret, J. C., Ryckelynck, M. and Griffiths, A. D., "A Completely in vitro Ultrahigh-throughput Droplet-based Microfluidic Screening System for Protein Engineering and Directed Evolution," Lab Chip, 12(5), 882-891(2012). crossref(new window)

74.
Tawfik, D. S. and Griffiths, A. D., "Man-made Cell-like Compartments for Molecular Evolution," Nat. Biotechnol., 16(7), 652-656(1998). crossref(new window)

75.
Juul, S., Nielsen, C. J. F., Labouriau, R., Roy, A., Tesauro, C., Jensen, P. W., Harmsen, C., Kristoffersen, E. L., Chiu, Y. L., Frohlich, R., Fiorani, P., Cox-Singh, J., Tordrup, D., Koch, J., Bienvenu, A. L., Desideri, A., Picot, S., Petersen, E., Leong, K. W., Ho, Y. P., Stougaard, M. and Knudsen, B. R., "Droplet Microfluidics Platform for Highly Sensitive and Quantitative Detection of Malaria-Causing Plasmodium Parasites Based on Enzyme Activity Measurement," ACS nano, 6(12), 10676-10683(2012). crossref(new window)

76.
Lee, K. G., Park, T. J., Soo, S. Y., Wang, K. W., Kim, B. H., Park, J. H., Lee, C. S., Kim, D. H. and Lee, S. J., "Synthesis and Utilization of E. coli-Encapsulated PEG-Based Microdroplet Using a Microfluidic Chip for Biological Application," Biotechnol. Bioeng., 107(4), 747-751(2010). crossref(new window)

77.
Joensson, H. N., Samuels, M. L., Brouzes, E. R., Medkova, M., Uhlen, M., Link, D. R. and Andersson-Svahn, H., "Detection and Analysis of Low-Abundance Cell-Surface Biomarkers Using Enzymatic Amplification in Microfluidic Droplets," Angew. Chem. Int. Edit., 48(14), 2518-2521(2009). crossref(new window)

78.
Brouzes, E., Medkova, M., Savenelli, N., Marran, D., Twardowski, M., Hutchison, J. B., Rothberg, J. M., Link, D. R., Perrimon, N. and Samuels, M. L., "Droplet Microfluidic Technology for Single-cell High-throughput Screening," Proc. Natl. Acad. Sci. USA, 106(34), 14195-14200(2009). crossref(new window)

79.
Edd, J. F., Carlo, D. D, Humphry, K. J., Koster, S., Irimia, D., Weitz, D. A. and Toner, M., "Controlled Encapsulation of Singlecells Into Monodisperse Picolitre Drops," Lab Chip, 8(8), 1262-1264(2008). crossref(new window)

80.
Kemna, E. W. M., Schoeman, R. M., Wolbers, F., Vermes, I., Weitz, D. A. and van den Berg, A., "High-yield Cell Ordering and Deterministic Cell-in-droplet Encapsulation Using Dean Flow in a Curved Microchannel," Lab Chip, 12(16), 2881-2887(2012). crossref(new window)

81.
Koster, S., Angile, F. E., Duan, H., Agresti, J. J., Wintner, A., Schmitz, C., Rowat, A. C., Merten, C. A., Pisignano, D., Griffiths, A. D. and Weitz, D. A., "Drop-based Microfluidic Devices for Encapsulation of Single Cells," Lab Chip, 8(7), 1110-1115(2008). crossref(new window)

82.
El Debs, B., Utharala, R., Balyasnikova, I. V., Griffiths, A. D. and Merten, C. A., "Functional Single-cell Hybridoma Screening Using Droplet-based Microfluidics," Proc. Natl. Acad. Sci. USA, 109(29), 11570-11575(2012). crossref(new window)