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Optimization of Fermentation Medium for Glycyrrhizin Biotransformation to Monoglucuronyl-glycyrrhetinic Acid by Plackett-Burman and Box-Behnken Design
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  • Journal title : Korean Chemical Engineering Research
  • Volume 53, Issue 3,  2015, pp.321-326
  • Publisher : The Korean Institute of Chemical Engineers
  • DOI : 10.9713/kcer.2015.53.3.321
 Title & Authors
Optimization of Fermentation Medium for Glycyrrhizin Biotransformation to Monoglucuronyl-glycyrrhetinic Acid by Plackett-Burman and Box-Behnken Design
Quan, Yanling; Wang, Lin; Liu, Yisheng; Cong, Jingxiang; Xie, Shengquan; Wu, Xiuhong;
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Plackett-Burman (PB) and Box-Behnken (BB) experimental designs were used to optimize fermentation variables for the biotransformation of glycyrrhizin (GL) to monoglucuronyl-glycyrrhetinic acid (MGGA). The PB design was first used to screen the important factors among the medium variables. The steepest ascent method was used to approach the optimum range for each of these factors. The BB design was finally used to analyze the response surfaces of the screened factors for further optimization. The optimized conditions for this system were 0.7 g/L , 1.19 g/L GL, and cultivation for six days. The biotransformation of GL to MGGA could reach up to 35.72%, which is a good result for this kind of transformation.
Glycyrrhizin;Aspergillus niger;Plackett-Burman Design;Biotransformation;Box-Behnken Design;
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Korean Chemical Engineering Research, 2016. vol.54. 6, pp.812-816 crossref(new window)
Yun, T. K. and Choi, S. Y., "Preventive Effect of Ginseng Intake Against Various Human Cancers: A Case-Control Study on 1987 Pairs," Cancer. Epidem. Biomar., 4(4), 401-408(1995).

Rudakewich, M., Ba, F. and Benishin, C. G., "Neurotrophic and Neuroprotective Actions of Ginsenosides Rb1and Rg1," Planta. Med., 67, 533-537(2001). crossref(new window)

Peng, L., Sun, S., Xie, L. H., Wicks, S. M. and Xie, J. T., "Ginsenoside Re: Pharmacological Effects on Cardiovascular System," Cardiovasc. Ther., 30(4), 183-188(2002).

Wang, Z. X., Song, J. L. and Yang, G. Z., "Current status of Study on Glycyrrhizin," Chinese. J. Integr. Tradit. West. Med., 22, 796-798(2002).

Cong, J. X. and Lin, B. C., "Separation of Liquiritin by Simulated Moving Bed Chromatography," J. Chromatogr. A., 1145, 190-194 (2007). crossref(new window)

Wang, X. and Zhang, H. I., "Advances in Studies on Pharmacological Activities of Ginkgolides," Chinese. Tradit. Herbal. Drugs. 36(11), 1741-1744(2005).

Xia, S. H. and Fang, D. C., "Pharmacological Action and Mechanisms of Ginkgolides B," Chinese. Med. J., 120(10), 922-928 (2007).

Clarke, K., Lee, F. T., Brechbiel, M. W., Smyth, F. E., Old, L. J., and Scott, A. M., "Therapeutic Efficacy of Anti-Lewisy Humanized 3S193 Radioimmunotherapy in a Breast Cancer Model: Enhanced Activity When Combined with Taxol Chemotherapy," Clin. Cancer. Res., 6, 3621-3628(2000).

Hou, J. G., Xue, J. J., Wang, C. Y., Liu, L., Zhang, D. L., Wang, Z., Li, W., Zheng, Y. N. and Sung, C. K., "Microbial Transformation of Ginsenoside Rg3 to Ginsenoside Rh2 by Esteya Vermicola CNU 120806," World. J. Microbiol. Biotechnol., 28, 1807-1811(2012). crossref(new window)

Kim, H. S., Lee, E. H., Ko, S. R., Choi, K. J., Park, J. H. and Im, D. S., "Effects of Ginsenosides Rg3 and Rh2 on the Proliferation of Prostate Cancer Cells," Arch. Pharrn.Res. 27(4), 429-435(2004). crossref(new window)

Sirikantaramas, S., Asano, T., Sudo, H., Yamazaki, M. and Saito, K., "Camptothecin: Therapeutic Potential and Biotechnology," Curr. Pharm. Biotechno., 8, 196-202(2007). crossref(new window)

Liu, Y. C., Chen, G. Y., Ge, F. L., Li, W., Zeng, L. H. and Cao, W. G., "Efficient Biotransformation of Cholesterol to Androsta- 1,4-diene-3,17-dione by a Newly Isolated Actinomycete Gordonia Neofelifaecis," World. J. Microbiol. Biotechnol. 27, 759-765 (2011). crossref(new window)

Gouiric, S. C., Feresin, G. E., Tapia, A. A., Rossomando, P. C., Schmeda-Hirschmann, G. and Bustos, D. A., "7b-Dihydroxydehydroabietic acid, a New Biotransformation Product of Dehydroabietic Acid by Aspergillus Niger," World. J. Microbiol. Biotechnol. 20, 281-284(2004). crossref(new window)

Hu, S. C., Hong, K., Song, Y. C., Liu, J. Y. Tan, R. X., "Biotransformation of Soybean Isoflavones by a Marine Streptomyces sp. 060524 and Cytotoxicity of the Products," World. J. Microbiol. Biotechnol., 25, 115-121(2009). crossref(new window)

Zou, S. P., Zhou, J. J., Kaleem, I., Xie, L. P., Liu, G. Y., Li, C., "Preparative Enrichment and Separation of Glycyrrhetinic Acid Monoglucuronide from Fermentation Broths with Macroporous Resins," Sep. Sci Technol., 47, 1055-1062(2012). crossref(new window)

Li, Y., Liu, Z. Q., Cui, F. J., Liu, Z. S. and Zhao, H., "Application of Plackett-Burman Experimental Design and Doehlert Design to Evaluate Nutritional Requirements for Xylanase Production by Alter Naria Mali ND-16," Appl. Microbiol. Biotechnol. 77, 285-291(2007). crossref(new window)

Palvannan, T. and Sathishkumar, P., "Production of Laccase from Pleurotus florida NCIM 1243using Plackett-Burman Design and Response Surface Methodology," J. Basic. Microb. 50, 325-335 (2010). crossref(new window)

Plackett, R. L. and Burman, J. P., "The Design of Optimum Multifactorial Experiments," Biometrika, 37, 305-325(1946).

Jacques, P., Hbid, C., Destain, J., Razafindralambo, H., Paquot, M., Pauw, E. D. and Thonart, P., "Optimization of Biosurfactant Lipopeptide Production from Bacillus subtilis S499 by Plackett-Burman Design," Appl. Biochem. Biotechnol., 77-79, 223-233 (1999).

Yue, P. F., Zheng, Q., Wu, B., Hu, P. Y., Wu, Z. F. and Yang, M., "Application of Plackett-Burman Design and Box-Behnken Design to Achieve Process Optimization for Geniposide Submicron Emulsion," J. Dispersion Sci. Technol., 33, 213-222(2012). crossref(new window)

Mahdavi, V. and Monajem, A., "Statistical Optimization for Oxidation of Ethyl Benzene over Co- Mn/SBA-15 catalyst by Box-Behnken Design," Korean J. Chem. Eng., 33, 2178-2185(2013).

Wu, S. J., Yong, Z. J., Zhu, L. H. and Jin, F. X., "Study on Biotransformation of Glycyrrhizin," Chinese. Tradit. Herbal. Drugs. 34, 516-518(2003).