Advanced SearchSearch Tips
Extraction of Genistein from Sophora flavescens with Supercritical Carbon Dioxide
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
  • Journal title : Korean Chemical Engineering Research
  • Volume 53, Issue 4,  2015, pp.445-449
  • Publisher : The Korean Institute of Chemical Engineers
  • DOI : 10.9713/kcer.2015.53.4.445
 Title & Authors
Extraction of Genistein from Sophora flavescens with Supercritical Carbon Dioxide
Han, Chang-Nam; Kang, Choon-Hyoung;
  PDF(new window)
This study was directed to finding an optimum extraction condition of genistein from the S. flavescens with supercritical carbon dioxide as a solvent. In this effort, effects of the extraction conditions including pressure, temperature and a co-solvent on the extraction efficiency were investigated. The aqueous ethanol and methanol solutions were used as co-solvents while the tested operating pressure and temperature ranges were from 200 bar to 300 bar and from 308.15 K to 323.15 K, respectively. The concentration of genistein was determined by means of HPLC equipped with a UV detector. From the results, it was observed that an increase in pressure led to the higher extraction efficiency. Further, methanol showed better performance as a co-slovent than ethanol. The DPPH radical scavenging activities were measured to compare antioxidant activities of S. flavescens extracts.
Genistein;S. flavescens;Supercritical Extraction;Co-solvent;Radical Scavenging Activity;
 Cited by
Lee, Y. W., "Supercritical Fluid Technology (I)," News Inf. Chem. Eng., 19(3), 325-333(2001).

Lee, Y. W., "Supercritical Fluid Technology (II)," News Inf. Chem. Eng., 19(4), 457-467(2001).

Dobbs, J. M., Wong, J. M., Lahiere, R. J. and Johnston, K. P., "Modification of Supercritical Fluid Phase Behavior Using Polar Cosolvents," Ind. Eng. Chem. Res., 26, 56-65(1987). crossref(new window)

Lucien, F. P. and Foster, N. R., "Solubilities of Solid Mixtures in Supercritical Carbon Dioxide: A Review," J. Supercrit. Fluids, 17, 111-134(2000). crossref(new window)

Mchugh, M. A. and Krukonis, V. J., Supercritical Fluid Extraction Principles and Practice, Butterworth-Heinemann, United States, US(1986).

Shin, M. K., "Extract of Sophorae Radix for the Prevention and Treatment of Osteoporosis," K. R. Patent No. 2002-0044746(2000).

Kelly, G. E., "Treatment or Prevention of Menopausal Symptoms and Osteoporosis," U.S. Patent No. 2002/0035074A1(2000).

Wang, G., Kuan, S. S., Francis, O. J. and Carman, S., "A Simplified HPLC Method for the Determination of Phtoestrogens in Soybean and Its Processed Procducts," J. Agric. Food Chem., 38, 185-190(1990). crossref(new window)

Peterson, G. and Barnes, S., "Genistein Inhibition of the Growth of Human Breast Cancer Cells: Independence from Estrogen Receptors and the Multi-Drug Resistance Gene," Biochem. Biophs. Res. Comm., 179, 661-667(1991). crossref(new window)

Coward, L., Barnes, N. C., Setchell, K. D. R. and Barnes, S., "Genistein, Daidzein and Their $\beta$-Glycoside Conjugates: Antitumor Isoflavone in Soybean Foods from American and Asian Diets," J. Agric. Food Chem., 41, 661-667(1961).

Supko, J. G. and Phillips, L. R., "High Performance Liquid Chromatographic Assay for Genistein in Biological Fluids," J. Chromatogr., 666, 157-167.

Levitzki, A and Gazit, A., "Tyrosine Kinase Inhibition: An Approach to Drug Development," Science, 267, 1782-1787(1995). crossref(new window)

Jyong, M. E. and Hwang, I. K., "Functional Components and Antioxidative Activities of Soybean Extracts," Korean Soybean Digest, 25(1), 23-29(2008).

So, E. H., Kuh, J. H., Park, K. Y. and Lee, Y. H., "Vrietal Difference of Isoflavone Content and Antioxidant Activity in Soybean," Korean J. Bread, 33(1), 35-39(2001).

Record, I. R., Dreosti, I. E. and Mclnerney, J. K., "The Antioxidant Activity of Genistein in vitro," National Biochem., 6, 481-485(1995).

Yang, E. S., Hong, R. H. and Kang, S. M., "The Effects of Genistein on the Proliferation and Type I pN Collagen Synthesis in Aged Normal Human Fibroblasts," Korean J. Microbiol. Biotechnol., 35(4), 316-324(2007).

Oh, H. S., Kim, J. H. and Lee, M. H., "Isoflavone Contents, Antioxidative and Fibrinolytic Activities of Red Bean and Mung Bean," Korean J. Soc. Food Cookepy SCI., 19(3), 263-270(2003).

Barnes, S. and Blair, H. C., "Genistein for Use in Inhibiting Osteroclasts," U.S. Patent No. 5, 506, 211(1996).

Blois, M. S., "Antioxidant Determination by the Use of Stable Free Radical," Nature, 181, 1199-1200(1958). crossref(new window)

Myung, J. E. and Hwang, I. K., "Functional Components and Antioxidative Activities of Soybean Extracts," Korean Soybean Digest, 25(1), 23-29(2008).

Jun, H. I., Denis, P., Wiesenborn and Kim, Y. S., "Antioxidant Activities of Various Solvent Extracts from Canola Meal," Korean J. Food Preserv, 18(1), 59-64(2011). crossref(new window)

Jeong, G. T., Lee, K. M. and Park, D. H., "Study of Antimicrobial and Antioxidant activities of Rumex crispus Extract," Korean Chem. Eng. Res., 44(1), 81-86(2006).