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Optimization of Medium for Protease Production by Enterobacteriaceae sp. PAMC 25617 by Response Surface Methodology
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  • Journal title : Korean Chemical Engineering Research
  • Volume 53, Issue 4,  2015, pp.524-529
  • Publisher : The Korean Institute of Chemical Engineers
  • DOI : 10.9713/kcer.2015.53.4.524
 Title & Authors
Optimization of Medium for Protease Production by Enterobacteriaceae sp. PAMC 25617 by Response Surface Methodology
Kim, Hyun-do; Yun, Chul-Won; Choi, Jong-il; Han, Se Jong;
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 Abstract
This study was conducted to optimize the medium composition for cold-adaptive protease production of Enterobacteriaceae sp. by response surface methodology (RSM). Yeast extract, and TritonX-100 were identified as the significant factors affecting protease from one-factor-at-a-time method. RSM studies for optimizing protease production of Enterobacteriaceae sp. have been carried out for three parameters including yeast extract concentration, TritonX-100 concentration, and culture pH. These significant factors were optimized as 6.690 g/L yeast extract, 0.018 g/L Triton X-10, and pH 6.677. The experimentally obtained protease activity was 8.03 U /L, and it became 1.5-fold increase before optimization.
 Keywords
Antarctic Microorganism;Protease;Response Surface Methodology;
 Language
Korean
 Cited by
1.
반응표면분석법을 통한 Arthrobacter sp.의 amylase 생산 최적화,김현도;임영금;최종일;한세종;

Korean Chemical Engineering Research, 2016. vol.54. 1, pp.140-144 crossref(new window)
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