Advanced SearchSearch Tips
Optimization of Medium for Protease Production by Enterobacteriaceae sp. PAMC 25617 by Response Surface Methodology
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
  • Journal title : Korean Chemical Engineering Research
  • Volume 53, Issue 4,  2015, pp.524-529
  • Publisher : The Korean Institute of Chemical Engineers
  • DOI : 10.9713/kcer.2015.53.4.524
 Title & Authors
Optimization of Medium for Protease Production by Enterobacteriaceae sp. PAMC 25617 by Response Surface Methodology
Kim, Hyun-do; Yun, Chul-Won; Choi, Jong-il; Han, Se Jong;
  PDF(new window)
This study was conducted to optimize the medium composition for cold-adaptive protease production of Enterobacteriaceae sp. by response surface methodology (RSM). Yeast extract, and TritonX-100 were identified as the significant factors affecting protease from one-factor-at-a-time method. RSM studies for optimizing protease production of Enterobacteriaceae sp. have been carried out for three parameters including yeast extract concentration, TritonX-100 concentration, and culture pH. These significant factors were optimized as 6.690 g/L yeast extract, 0.018 g/L Triton X-10, and pH 6.677. The experimentally obtained protease activity was 8.03 U /L, and it became 1.5-fold increase before optimization.
Antarctic Microorganism;Protease;Response Surface Methodology;
 Cited by
반응표면분석법을 통한 Arthrobacter sp.의 amylase 생산 최적화,김현도;임영금;최종일;한세종;

Korean Chemical Engineering Research, 2016. vol.54. 1, pp.140-144 crossref(new window)
Nichols, D., Bowman, J., Sanderson, K., Nichols, C. M., Lewis, T., McMeekin, T. and Nichols, P. D., "Developments with Antarctic Microorganisms: Culture Collections, Bioactivity Screening, Taxonomy, PUFA Production and Cold-adapted Enzymes," Curr. Opin. Biotechnol." 10, 240-246(1999). crossref(new window)

Antranikian, G. and Egorova, K., "Extremophiles, a Unique Resource of Biocatalysts for Industrial Biotechnology," Physiology and Biochemistry of Extremophiles. ASM Press. Washington, 361-406(2007).

Huston, A. L., "Biotechnological Aspects of Cold-adapted Enzymes," In Psychrophiles: From Biodiversity to Biotechnology. Springer. Berlin. Heidelberg, 347-363(2008).

Kim, D. K., Park, H. J., Lee, Y. M., Hong, S. G., Lee, H. K. and Yim, J. H., "Screening for Cold-Active Protease-Producing Bacteria from the Culture Collection of Polar Microorganisms and Characterization of Proteolytic Activities," Korean J. Microbiol., 46, 73-79(2010).

Zhu, H. Y., Tian, Y., Hou, Y. H. and Wang, T. H., "Purification and Characterization of the Cold-active Alkaline Protease from Marine Cold-adaptive Penicillium Chrysogenum FS010," Mol. Boil. rep., 36, 2169-2174(2009). crossref(new window)

Georlette, D., Blaise, V., Collins, T., D'Amico, S., Gratia, E., Hoyoux, A., Marx, J., Sonan, G., Feller, G. and Gerday, C., "Some Like it Cold: Biocatalysis at Low Temperatures," FEMS microbial. Rev., 28, 25-42(2004).

Yang, J., Li, J., Mai, Z., Tian, X. and Zhang, S., "Purification, Characterization, and Gene Cloning of a Cold-adapted Thermolysin-Like Protease from Halobacillus sp. SCSIO 20089," J. Biosci. Bioeng., 115, 628-632(2013). crossref(new window)

Gerday, C., Aittaleb, M., Bentahir, M., Chessa, J. P., Claverie, P., Collins, T., D'Amico, S., Dumont, J., Garsoux, G., Georlette, D., Hoyoux, A., Lonhienne, T., Meuwis, M. A. and Feller G., "Coldadapted Enzymes: from Fundamentals to Biotechnology," Trends. Biotechnol., 18, 103-107(2000). crossref(new window)

Dastager, S. G., Dayanand, A., Li, W. J., Kim, C. J., Lee, J. C., Park, D. J., Tian, X. P. and Raziuddin, Q.S., "Proteolytic Activity from An Alkali-thermotolerant Streptomyces gulbargensis sp. nov," Curr. Microbiol., 57, 638-642(2008). crossref(new window)

Kirk, O., Borchert, T. V. and Fuglsang, C. C., "Industrial Enzyme Applications," Curr. Opin. Biotechnol., 13, 345-351(2002). crossref(new window)

Cherry, J. R. and Fidantsef, A. L., "Directed Evolution of Industrial Enzymes: An Update," Curr. Opin. Biotechnol., 14, 438-443(2003).

Daniel R. M., Toogood, H. S. and Bergquist, P. L., "Thermostable Proteases," Biotech. Genet. Eng. Rev., 13, 51-100(1996). crossref(new window)

Gupta, R., Beg, Q. and Lorenz, P., "Bacterial Alkaline Proteases: Molecular Approaches and Industrial Applications," Appl. Microbial. Biotechnol., 59, 15-32(2002). crossref(new window)

Anisworth, S. J., "Soap & Detergents," Chem. Eng. News., 70, 27-37(1992).

Marx, J. C., Collins, T., D'Amico, S., Feller, G. and Gerday, C., "Cold-adapted Enzymes from Marine Antarctic Microorganisms," Marine Biotechnol., 9, 293-304(2007). crossref(new window)

Cavicchioli, R., Siddiqui, K. S., Andrews, D. and Sowers, K. R., "Low-temperature Extremophiles and Their Applications," Curr. Opin. Biotechnol., 13, 253-261(2002). crossref(new window)

Wang, Q., Hou, Y., Xu, Z., Miao, J. and Li, G., "Optimization of Cold-active Protease Production by the Psychrophilic Bacterium Colwellia sp. NJ341 with Response Surface Methodology," Biores. Technol., 99, 1926-1931(2008). crossref(new window)

Irwin, J. A., Alfredsson, G. A., Lanzetti, A. J., Gudmundsson, H. M. and Engel, P. C., "Purification and Characterisation of a Serine Peptidase from the Marine Psychrophile Strain PA-43," FEMS Microbiol. Let., 201, 285-290(2001). crossref(new window)

Siddiqui, K. S. and Cavicchioli, R., "Cold-adapted Enzymes," Annual Rev. Biochem., 75, 403-433(2006). crossref(new window)

Kackar, R. N., "Off-line Quality Control, Parameter Design, and the Taguchi Method," J. Quality Technol., 17, 176-188(1985). crossref(new window)

Ghani, J. A., Choudhury, I. A. and Hassan, H. H. "Application of Taguchi Method in the Optimization of End Milling Parameters," J. Mater. Process. Technol., 145, 84-92(2004). crossref(new window)

Tsai, J. T., Liu, T. K. and Chou, J. H. "Hybrid Taguchi-genetic Algorithm for Global Numerical Optimization," Evolutionary Computation, IEEE Transactions on, 8, 365-377(2004). crossref(new window)

Puri, S., Beg, Q. K. and Gupta, R., "Optimization of Alkaline Protease Production from Bacillus sp. By Response Surface Methodology," Curr, Microbiol., 44, 286-290(2002). crossref(new window)

Adinarayana, K. and Ellaiah, P., "Response Surface Optimization of the Critical Medium Components for the Production of Alka-Line Protease by a Newly Isolated Bacillus sp.," J. Pharmacy. Pharmaceutical Sci., 5, 272-278(2002).

Dutta, J. R., Dutta, P. K. and Banerjee, R., "Optimization of Culture Parameters for Extracellular Protease Production from a Newly Isolated Pseudomonas sp. Using Response Surface and Artificial Neural Network Models," Proc. Biochem., 39, 2193-2198(2004). crossref(new window)

Hanlon, G. W., Hodges, N. A. and Russel, A. D., "The Influence of Glucose, Ammonium and Magnesium Availability on the Production of Protease and Bacitracin by Bacillus Licheniformis," J. General Microbiol., 128, 845-851(1982).

Tabaraki, R. and Rastgoo, S., "Comparison Between Conventional and Ultrasound-assisted Extractions of Natural Antioxidants from Walnut Green Husk," Korean J. Chem. Eng., 31, 676-683(2014) crossref(new window)

İlbay, Z., Sahin, S. and Buyukkabasakal, K., "A Novel Approach for Olive Leaf Extraction Through Ultrasound Technology: Response Surface Methodology Versus Artificial Neural Networks," Korean J. Chem. Eng., 31, 1661-1667(2014). crossref(new window)

Toon, C. H., Bok, H. S., Choi, D. K. and Row, K. H., "Optimization Condition of Astaxanthin Extract from Shrimp Waste Using Response Surface Methodology," Korean Chem. Eng. Res., 50, 545-550(2012). crossref(new window)

Kin, J. W., "Response Surface Optimization of Fermentation Parameters for Citric Acid Production in Solid Substrate Fermentation," Korean Chem. Eng. Res., 50, 879-884(2012). crossref(new window)

Thys, R., Guzzon, S. O., Cladera-Olivera, F. and Brandelli, A., "Optimization of Protease Production by Microbacterium sp. in Feather Meal Using Response Surface Methodology," Proc. Biochem., 41, 67-73(2006). crossref(new window)

De Coninck, J., Bouquelet, S., Dumortier, V., Duyme, F. and Verdier-Denantes, I., "Industrial Media and Fermentation Process for Improved Growth and Protease Production by Tetrahymena thermophila BIII," J. Ind. Microbiol. Biotechnol., 24, 285-290(2000). crossref(new window)

Plotnick, M. I., Rubin, H. and Schechter, N. M., "The Effects of Reactive Site Location on the Inhibitory Properties of the Serpin ${\alpha}_{1}$ - Antichymotrypsin," J. Biol. Chem., 277, 29927-29935(2002). crossref(new window)

McKeller, R. C. and Cholette, H., "Synthesis of Extracellular Proteinase by Pseudomonas fluorescens Under Conditions of Limiting Carbon, Nitrogen and Phosphate," Appl. Biochem. Microbiol., 47, 1224-1227(1984).